RT-qPCR-based pool testing for the diagnosis of COVID-19

RT-qPCR-based pool testing for the diagnosis of COVID-19

ABSTRACT This study proposes a strategy for large-scale testing among a large number of people for the early diagnosis of COVID-19 to elucidate the epidemiological situation. Pool testing involves the analysis of pooled samples. This study aimed to discuss a reverse transcription technique followed...

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Bibliographic Details
Main Authors: Hugo Itaru Sato, Murilo Soares Costa, Ricardo Hiroshi Caldeira Takahashi, Karine Lima Lourenço, Nathalia Sernizon Guimarães, Claudia Regina Lindgren Alves, Elaine Leandro Machado, Unaí Tupinambás, Flávio Guimarães da Fonseca, Santuza Maria Ribeiro Teixeira
Format: Article
Language:English
Published: Instituto Israelita de Ensino e Pesquisa Albert Einstein 2023-07-01
Series:Einstein (São Paulo)
Subjects:
COVID-19
Coronavirus infections
SARS-CoV-2
Pandemics
Reverse transcriptase polymerase chain reaction
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082023000100900&lng=en&tlng=en
Description
Summary:ABSTRACT This study proposes a strategy for large-scale testing among a large number of people for the early diagnosis of COVID-19 to elucidate the epidemiological situation. Pool testing involves the analysis of pooled samples. This study aimed to discuss a reverse transcription technique followed by quantitative real-time polymerase chain reaction using pool testing to detect SARS-CoV-2 in nasopharyngeal swab samples. The study proposes an innovative diagnostic strategy that contributes to resource optimization, cost reduction, and improved agility of feedback from results.
ISSN:2317-6385

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