Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell Levels
Colorectal neoplasia differentially expressed (CRNDE) is an oncogenic long noncoding RNA (lncRNA) overexpressed in diverse malignancies. Here, we comprehensively analyze the prognostic value and molecular function of CRNDE in glioma. Bulk RNA-sequencing data from The Cancer Genome Atlas (TCGA) and C...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/22/3669 |
| _version_ | 1797465671062257664 |
|---|---|
| author | Lairong Song Xiaojie Li Xiaoying Xu Xulei Huo Yi Zheng Xiaomin Wang Da Li Junting Zhang Ke Wang Liang Wang Zhen Wu |
| author_facet | Lairong Song Xiaojie Li Xiaoying Xu Xulei Huo Yi Zheng Xiaomin Wang Da Li Junting Zhang Ke Wang Liang Wang Zhen Wu |
| author_sort | Lairong Song |
| collection | DOAJ |
| description | Colorectal neoplasia differentially expressed (CRNDE) is an oncogenic long noncoding RNA (lncRNA) overexpressed in diverse malignancies. Here, we comprehensively analyze the prognostic value and molecular function of CRNDE in glioma. Bulk RNA-sequencing data from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA), and single-cell RNA-sequencing data from the Tumor Immune Single-Cell Hub (TISCH) were analyzed. Kaplan–Meier survival analysis was applied to verify the prognostic value of CRNDE. Then, a nomogram based on multivariate Cox regression was established for individualized survival prediction. Subsequently, the expression characteristic and biological function of CRNDE were analyzed at the single-cell level. Lastly, the effects of CRNDE on the proliferation and invasion of glioma cell were explored in vitro. We discovered that CRNDE was a powerful marker for risk stratification of glioma patients. Regardless of the status of IDH and 1p/19q, CRNDE could effectively stratify patients’ prognosis. The nomogram that incorporated the CRNDE expression was proved to be a reliable tool for survival prediction. In addition, epithelial–mesenchymal transition may be the most important biological process regulated by CRNDE, which was identified at both the bulk and single-cell levels. Moreover, CRNDE knockdown significantly inhibited the proliferation and invasion of glioma cell. Overall, CRNDE is a vital oncogene and may be a valuable supplement to improve the clinical stratification of glioma. |
| first_indexed | 2024-03-09T18:24:51Z |
| format | Article |
| id | doaj.art-d087d56160384a8c9ffd6121fb8e99be |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T18:24:51Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-d087d56160384a8c9ffd6121fb8e99be2023-11-24T07:59:08ZengMDPI AGCells2073-44092022-11-011122366910.3390/cells11223669Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell LevelsLairong Song0Xiaojie Li1Xiaoying Xu2Xulei Huo3Yi Zheng4Xiaomin Wang5Da Li6Junting Zhang7Ke Wang8Liang Wang9Zhen Wu10Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Lymphoma, Peking University Cancer Hospital & Institute, Beijing 100089, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100071, ChinaColorectal neoplasia differentially expressed (CRNDE) is an oncogenic long noncoding RNA (lncRNA) overexpressed in diverse malignancies. Here, we comprehensively analyze the prognostic value and molecular function of CRNDE in glioma. Bulk RNA-sequencing data from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA), and single-cell RNA-sequencing data from the Tumor Immune Single-Cell Hub (TISCH) were analyzed. Kaplan–Meier survival analysis was applied to verify the prognostic value of CRNDE. Then, a nomogram based on multivariate Cox regression was established for individualized survival prediction. Subsequently, the expression characteristic and biological function of CRNDE were analyzed at the single-cell level. Lastly, the effects of CRNDE on the proliferation and invasion of glioma cell were explored in vitro. We discovered that CRNDE was a powerful marker for risk stratification of glioma patients. Regardless of the status of IDH and 1p/19q, CRNDE could effectively stratify patients’ prognosis. The nomogram that incorporated the CRNDE expression was proved to be a reliable tool for survival prediction. In addition, epithelial–mesenchymal transition may be the most important biological process regulated by CRNDE, which was identified at both the bulk and single-cell levels. Moreover, CRNDE knockdown significantly inhibited the proliferation and invasion of glioma cell. Overall, CRNDE is a vital oncogene and may be a valuable supplement to improve the clinical stratification of glioma.https://www.mdpi.com/2073-4409/11/22/3669CRNDEgliomaprognostic modelsingle-cell RNA sequencingepithelial–mesenchymal transition |
| spellingShingle | Lairong Song Xiaojie Li Xiaoying Xu Xulei Huo Yi Zheng Xiaomin Wang Da Li Junting Zhang Ke Wang Liang Wang Zhen Wu Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell Levels Cells CRNDE glioma prognostic model single-cell RNA sequencing epithelial–mesenchymal transition |
| title | Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell Levels |
| title_full | Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell Levels |
| title_fullStr | Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell Levels |
| title_full_unstemmed | Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell Levels |
| title_short | Comprehensive Analysis of the Prognostic Value and Molecular Function of CRNDE in Glioma at Bulk and Single-Cell Levels |
| title_sort | comprehensive analysis of the prognostic value and molecular function of crnde in glioma at bulk and single cell levels |
| topic | CRNDE glioma prognostic model single-cell RNA sequencing epithelial–mesenchymal transition |
| url | https://www.mdpi.com/2073-4409/11/22/3669 |
| work_keys_str_mv | AT lairongsong comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT xiaojieli comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT xiaoyingxu comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT xuleihuo comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT yizheng comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT xiaominwang comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT dali comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT juntingzhang comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT kewang comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT liangwang comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels AT zhenwu comprehensiveanalysisoftheprognosticvalueandmolecularfunctionofcrndeingliomaatbulkandsinglecelllevels |