Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones
We sought to identify and quantitatively analyze calcium oxalate (CaOx) kidney stones on the order of micrometers, with a focus on the quantitative identification of calcium oxalate monohydrate (COM) and dihydrate (COD). We performed Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffr...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9997882/?tool=EBI |
| _version_ | 1811154286791360512 |
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| author | Mihoko Maruyama Koichi P. Sawada Yutaro Tanaka Atsushi Okada Koichi Momma Masanori Nakamura Ryota Mori Yoshihiro Furukawa Yuki Sugiura Rie Tajiri Kazumi Taguchi Shuzo Hamamoto Ryosuke Ando Katsuo Tsukamoto Kazufumi Takano Masayuki Imanishi Masashi Yoshimura Takahiro Yasui Yusuke Mori |
| author_facet | Mihoko Maruyama Koichi P. Sawada Yutaro Tanaka Atsushi Okada Koichi Momma Masanori Nakamura Ryota Mori Yoshihiro Furukawa Yuki Sugiura Rie Tajiri Kazumi Taguchi Shuzo Hamamoto Ryosuke Ando Katsuo Tsukamoto Kazufumi Takano Masayuki Imanishi Masashi Yoshimura Takahiro Yasui Yusuke Mori |
| author_sort | Mihoko Maruyama |
| collection | DOAJ |
| description | We sought to identify and quantitatively analyze calcium oxalate (CaOx) kidney stones on the order of micrometers, with a focus on the quantitative identification of calcium oxalate monohydrate (COM) and dihydrate (COD). We performed Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (PXRD), and microfocus X-ray computed tomography measurements (microfocus X-ray CT) and compared their results. An extended analysis of the FTIR spectrum focusing on the 780 cm−1 peak made it possible to achieve a reliable analysis of the COM/COD ratio. We succeeded in the quantitative analysis of COM/COD in 50-μm2 areas by applying microscopic FTIR for thin sections of kidney stones, and by applying microfocus X-ray CT system for bulk samples. The analysis results based on the PXRD measurements with micro-sampling, the microscopic FTIR analysis of thin sections, and the microfocus X-ray CT system observation of a bulk kidney stone sample showed roughly consistent results, indicating that all three methods can be used complementarily. This quantitative analysis method evaluates the detailed CaOx composition on the preserved stone surface and provides information on the stone formation processes. This information clarifies where and which crystal phase nucleates, how the crystals grow, and how the transition from the metastable phase to the stable phase proceeds. The phase transition affects the growth rate and hardness of kidney stones and thus provides crucial clues to the kidney stone formation process. |
| first_indexed | 2024-04-10T04:14:13Z |
| format | Article |
| id | doaj.art-d0896078e4d1470abf92bb72349ec83e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T04:14:13Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d0896078e4d1470abf92bb72349ec83e2023-03-12T05:32:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01183Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stonesMihoko MaruyamaKoichi P. SawadaYutaro TanakaAtsushi OkadaKoichi MommaMasanori NakamuraRyota MoriYoshihiro FurukawaYuki SugiuraRie TajiriKazumi TaguchiShuzo HamamotoRyosuke AndoKatsuo TsukamotoKazufumi TakanoMasayuki ImanishiMasashi YoshimuraTakahiro YasuiYusuke MoriWe sought to identify and quantitatively analyze calcium oxalate (CaOx) kidney stones on the order of micrometers, with a focus on the quantitative identification of calcium oxalate monohydrate (COM) and dihydrate (COD). We performed Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (PXRD), and microfocus X-ray computed tomography measurements (microfocus X-ray CT) and compared their results. An extended analysis of the FTIR spectrum focusing on the 780 cm−1 peak made it possible to achieve a reliable analysis of the COM/COD ratio. We succeeded in the quantitative analysis of COM/COD in 50-μm2 areas by applying microscopic FTIR for thin sections of kidney stones, and by applying microfocus X-ray CT system for bulk samples. The analysis results based on the PXRD measurements with micro-sampling, the microscopic FTIR analysis of thin sections, and the microfocus X-ray CT system observation of a bulk kidney stone sample showed roughly consistent results, indicating that all three methods can be used complementarily. This quantitative analysis method evaluates the detailed CaOx composition on the preserved stone surface and provides information on the stone formation processes. This information clarifies where and which crystal phase nucleates, how the crystals grow, and how the transition from the metastable phase to the stable phase proceeds. The phase transition affects the growth rate and hardness of kidney stones and thus provides crucial clues to the kidney stone formation process.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9997882/?tool=EBI |
| spellingShingle | Mihoko Maruyama Koichi P. Sawada Yutaro Tanaka Atsushi Okada Koichi Momma Masanori Nakamura Ryota Mori Yoshihiro Furukawa Yuki Sugiura Rie Tajiri Kazumi Taguchi Shuzo Hamamoto Ryosuke Ando Katsuo Tsukamoto Kazufumi Takano Masayuki Imanishi Masashi Yoshimura Takahiro Yasui Yusuke Mori Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones PLoS ONE |
| title | Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones |
| title_full | Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones |
| title_fullStr | Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones |
| title_full_unstemmed | Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones |
| title_short | Quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones |
| title_sort | quantitative analysis of calcium oxalate monohydrate and dihydrate for elucidating the formation mechanism of calcium oxalate kidney stones |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9997882/?tool=EBI |
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