Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes
Gold nanorods (AuNRs) have been proposed to promote stem cell differentiation in vitro and in vivo. In this study, we examined a particular type of AuNR in supporting the differentiation of rat fetal neural stem cells (NSCs) into oligodendrocytes (ODCs). AuNRs were synthesized according to the seed-...
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MDPI AG
2022-03-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/6/929 |
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| author | Krishna Deo Sharma Karrer M. Alghazali Rabab N. Hamzah Sahitya Chetan Pandanaboina Zeid A. Nima Alsudani Malek Muhi Fumiya Watanabe Guo-Lei Zhou Alexandru S. Biris Jennifer Yanhua Xie |
| author_facet | Krishna Deo Sharma Karrer M. Alghazali Rabab N. Hamzah Sahitya Chetan Pandanaboina Zeid A. Nima Alsudani Malek Muhi Fumiya Watanabe Guo-Lei Zhou Alexandru S. Biris Jennifer Yanhua Xie |
| author_sort | Krishna Deo Sharma |
| collection | DOAJ |
| description | Gold nanorods (AuNRs) have been proposed to promote stem cell differentiation in vitro and in vivo. In this study, we examined a particular type of AuNR in supporting the differentiation of rat fetal neural stem cells (NSCs) into oligodendrocytes (ODCs). AuNRs were synthesized according to the seed-mediated method resulting in nanorods with an aspect ratio of around 3 (~12 nm diameter, 36 nm length) and plasmon resonance at 520 and 780 nm, as confirmed by transmission electron microscopy (TEM) and UV-vis spectroscopy, respectively. A layer-by-layer approach was used to fabricate the AuNR substrate on the functionalized glass coverslips. NSCs were propagated for 10 days using fibroblast growth factor, platelet-derived growth-factor-supplemented culture media, and differentiated on an AuNR or poly-D-lysine (PDL)-coated surface using differentiation media containing triiodothyronine for three weeks. Results showed that NSCs survived better and differentiated faster on the AuNRs compared to the PDL surface. By week 1, almost all cells had differentiated on the AuNR substrate, whereas only ~60% differentiated on the PDL surface, with similar percentages of ODCs and astrocytes. This study indicates that functionalized AuNR substrate does promote NSC differentiation and could be a viable tool for tissue engineering to support the differentiation of stem cells. |
| first_indexed | 2024-03-09T13:08:02Z |
| format | Article |
| id | doaj.art-d08d3d592b1246118afee5a9328b8390 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T13:08:02Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-d08d3d592b1246118afee5a9328b83902023-11-30T21:45:41ZengMDPI AGNanomaterials2079-49912022-03-0112692910.3390/nano12060929Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into OligodendrocytesKrishna Deo Sharma0Karrer M. Alghazali1Rabab N. Hamzah2Sahitya Chetan Pandanaboina3Zeid A. Nima Alsudani4Malek Muhi5Fumiya Watanabe6Guo-Lei Zhou7Alexandru S. Biris8Jennifer Yanhua Xie9Molecular Biosciences Graduate Program, Arkansas State University, State University, AR 72467, USACenter for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USACenter for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USAArkansas Biosciences Institute, Arkansas State University, Jonesboro, AR 72401, USACenter for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USACenter for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USACenter for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USAMolecular Biosciences Graduate Program, Arkansas State University, State University, AR 72467, USACenter for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, AR 72204, USAMolecular Biosciences Graduate Program, Arkansas State University, State University, AR 72467, USAGold nanorods (AuNRs) have been proposed to promote stem cell differentiation in vitro and in vivo. In this study, we examined a particular type of AuNR in supporting the differentiation of rat fetal neural stem cells (NSCs) into oligodendrocytes (ODCs). AuNRs were synthesized according to the seed-mediated method resulting in nanorods with an aspect ratio of around 3 (~12 nm diameter, 36 nm length) and plasmon resonance at 520 and 780 nm, as confirmed by transmission electron microscopy (TEM) and UV-vis spectroscopy, respectively. A layer-by-layer approach was used to fabricate the AuNR substrate on the functionalized glass coverslips. NSCs were propagated for 10 days using fibroblast growth factor, platelet-derived growth-factor-supplemented culture media, and differentiated on an AuNR or poly-D-lysine (PDL)-coated surface using differentiation media containing triiodothyronine for three weeks. Results showed that NSCs survived better and differentiated faster on the AuNRs compared to the PDL surface. By week 1, almost all cells had differentiated on the AuNR substrate, whereas only ~60% differentiated on the PDL surface, with similar percentages of ODCs and astrocytes. This study indicates that functionalized AuNR substrate does promote NSC differentiation and could be a viable tool for tissue engineering to support the differentiation of stem cells.https://www.mdpi.com/2079-4991/12/6/929gold nanorodrat fetal neural stem cellsdifferentiationoligodendrocytes |
| spellingShingle | Krishna Deo Sharma Karrer M. Alghazali Rabab N. Hamzah Sahitya Chetan Pandanaboina Zeid A. Nima Alsudani Malek Muhi Fumiya Watanabe Guo-Lei Zhou Alexandru S. Biris Jennifer Yanhua Xie Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes Nanomaterials gold nanorod rat fetal neural stem cells differentiation oligodendrocytes |
| title | Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes |
| title_full | Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes |
| title_fullStr | Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes |
| title_full_unstemmed | Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes |
| title_short | Gold Nanorod Substrate for Rat Fetal Neural Stem Cell Differentiation into Oligodendrocytes |
| title_sort | gold nanorod substrate for rat fetal neural stem cell differentiation into oligodendrocytes |
| topic | gold nanorod rat fetal neural stem cells differentiation oligodendrocytes |
| url | https://www.mdpi.com/2079-4991/12/6/929 |
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