The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage
The objective of this study was to investigate the effect of lactic acid bacteria (LAB) inoculums on fermentation quality and in vitro digestibility of corn stover silage. Corn stover was ensiled without (control) or with Lactobacillus plantarum (LP), Enterococcus faecalis (EF), and Enterococcus mun...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-03-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311919627073 |
| _version_ | 1819001627938390016 |
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| author | Gang GUO Chen SHEN Qiang LIU Shuan-lin ZHANG Tao SHAO Cong WANG Yong-xin WANG Qing-fang XU Wen-jie HUO |
| author_facet | Gang GUO Chen SHEN Qiang LIU Shuan-lin ZHANG Tao SHAO Cong WANG Yong-xin WANG Qing-fang XU Wen-jie HUO |
| author_sort | Gang GUO |
| collection | DOAJ |
| description | The objective of this study was to investigate the effect of lactic acid bacteria (LAB) inoculums on fermentation quality and in vitro digestibility of corn stover silage. Corn stover was ensiled without (control) or with Lactobacillus plantarum (LP), Enterococcus faecalis (EF), and Enterococcus mundtii (EM) for 45 days. The fermentation characteristics were assessed, and subsequent in vitro dry matter digestibility (DM-D), neutral detergent fiber digestibility (NDF-D), volatile fatty acids (VFA), methane (CH4) production, cellulolytic bacteria proportions and their activities per corn stover silage were also determined. There was no significant difference (P>0.05) among the silage pH, lactic acid, crude protein (CP), water soluble carbohydrates (WSC) and lignocelluloses contents of different treatments. The relative proportions of Ruminococcus flavefaciens and Fibrobacter succinogenes, carboxymethyl-ocellulose and β-glycosidase activities, DM-D, NDF-D, and VFA production of in vitro incubation was higher (P<0.05) for silages inoculated with LP and EF than those of the control silage. Silage inoculated with LP showed the lowest (P<0.05) CH4 production per unit yield of VFA, which was positively corresponded to the lowest (P<0.05) ratio of acetate to propionate. In summary, the ensiling fermentation quality and subsequent utilization of corn stover silage were efficiently improved by inoculated with L. plantarum. |
| first_indexed | 2024-12-20T22:52:14Z |
| format | Article |
| id | doaj.art-d08d943e95154160812a89f3880a287e |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-20T22:52:14Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-d08d943e95154160812a89f3880a287e2022-12-21T19:24:12ZengElsevierJournal of Integrative Agriculture2095-31192020-03-01193838847The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silageGang GUO0Chen SHEN1Qiang LIU2Shuan-lin ZHANG3Tao SHAO4Cong WANG5Yong-xin WANG6Qing-fang XU7Wen-jie HUO8College of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.ChinaCollege of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.ChinaCollege of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.ChinaCollege of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.ChinaInstitute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaCollege of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.ChinaCollege of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.ChinaCollege of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.ChinaCollege of Animal Sciences and Veterinary Medicines, Shanxi Agricultural University, Taigu 030801, P.R.China; Correspondence HUO Wen-jie, Tel: +86-354-6287335, Fax: +86-354-6288052The objective of this study was to investigate the effect of lactic acid bacteria (LAB) inoculums on fermentation quality and in vitro digestibility of corn stover silage. Corn stover was ensiled without (control) or with Lactobacillus plantarum (LP), Enterococcus faecalis (EF), and Enterococcus mundtii (EM) for 45 days. The fermentation characteristics were assessed, and subsequent in vitro dry matter digestibility (DM-D), neutral detergent fiber digestibility (NDF-D), volatile fatty acids (VFA), methane (CH4) production, cellulolytic bacteria proportions and their activities per corn stover silage were also determined. There was no significant difference (P>0.05) among the silage pH, lactic acid, crude protein (CP), water soluble carbohydrates (WSC) and lignocelluloses contents of different treatments. The relative proportions of Ruminococcus flavefaciens and Fibrobacter succinogenes, carboxymethyl-ocellulose and β-glycosidase activities, DM-D, NDF-D, and VFA production of in vitro incubation was higher (P<0.05) for silages inoculated with LP and EF than those of the control silage. Silage inoculated with LP showed the lowest (P<0.05) CH4 production per unit yield of VFA, which was positively corresponded to the lowest (P<0.05) ratio of acetate to propionate. In summary, the ensiling fermentation quality and subsequent utilization of corn stover silage were efficiently improved by inoculated with L. plantarum.http://www.sciencedirect.com/science/article/pii/S2095311919627073corn stoverin vitro digestibilitylactic acid bacteriasilage |
| spellingShingle | Gang GUO Chen SHEN Qiang LIU Shuan-lin ZHANG Tao SHAO Cong WANG Yong-xin WANG Qing-fang XU Wen-jie HUO The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage Journal of Integrative Agriculture corn stover in vitro digestibility lactic acid bacteria silage |
| title | The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage |
| title_full | The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage |
| title_fullStr | The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage |
| title_full_unstemmed | The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage |
| title_short | The effect of lactic acid bacteria inoculums on in vitro rumen fermentation, methane production, ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage |
| title_sort | effect of lactic acid bacteria inoculums on in vitro rumen fermentation methane production ruminal cellulolytic bacteria populations and cellulase activities of corn stover silage |
| topic | corn stover in vitro digestibility lactic acid bacteria silage |
| url | http://www.sciencedirect.com/science/article/pii/S2095311919627073 |
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