Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (<i>Brassica oleracea</i> var. <i>acephala</i>)
Cut flower ornamental kale (<i>Brassica oleracea</i> var. <i>acephala</i>) is a biennial cultivar, which completes a sexual reproductive generation in two years. Isolated microspore culture (IMC) can accelerate plant homozygosity instead of self-pollinations. However, the app...
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MDPI AG
2022-08-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/9/790 |
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| author | Chuanhong Liu Gengxing Song Yonghui Zhao Bing Fang Zhiyong Liu Jie Ren Hui Feng |
| author_facet | Chuanhong Liu Gengxing Song Yonghui Zhao Bing Fang Zhiyong Liu Jie Ren Hui Feng |
| author_sort | Chuanhong Liu |
| collection | DOAJ |
| description | Cut flower ornamental kale (<i>Brassica oleracea</i> var. <i>acephala</i>) is a biennial cultivar, which completes a sexual reproductive generation in two years. Isolated microspore culture (IMC) can accelerate plant homozygosity instead of self-pollinations. However, the application of IMC in cut flower ornamental kale was rare since its low rate of embryogenesis. It is proved that histone acetylation might affect the gene expression in microspores and led to the transformation of microspores from pollen development pathway to embryogenesis. In this paper, microspores, derived from three varieties of cut flower ornamental kale, Crane Bicolor (CB), Crane Pink (CP) and Crane Feather Queen (CFQ), were treated with histone deacetylation inhibitor (HDACI) trichostatin A (TSA). Results revealed that the appropriate concentration of TSA was 10 nM for CB with obtaining 5.39 embryos per bud, while for CP and CFQ was 5 nM with acquiring 10.89 and 16.99 embryos per bud, respectively. TSA treatment also reduced the embryonic mortality, of which 10 nM TSA treatments CB was the optimal and the embryonic mortality decreased to 25.01%. The double haploid (DH) proportion of regenerated plants reached 37.3%. These results contribute to improving the technology for IMC in cut flower ornamental kale. |
| first_indexed | 2024-03-09T23:52:00Z |
| format | Article |
| id | doaj.art-d08e35c17d374804aebabe87bd5bf9ba |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T23:52:00Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-d08e35c17d374804aebabe87bd5bf9ba2023-11-23T16:33:41ZengMDPI AGHorticulturae2311-75242022-08-018979010.3390/horticulturae8090790Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (<i>Brassica oleracea</i> var. <i>acephala</i>)Chuanhong Liu0Gengxing Song1Yonghui Zhao2Bing Fang3Zhiyong Liu4Jie Ren5Hui Feng6Department of Horticulture, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, ChinaForeign Language Teaching Department, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, ChinaDepartment of Horticulture, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang 110866, ChinaCut flower ornamental kale (<i>Brassica oleracea</i> var. <i>acephala</i>) is a biennial cultivar, which completes a sexual reproductive generation in two years. Isolated microspore culture (IMC) can accelerate plant homozygosity instead of self-pollinations. However, the application of IMC in cut flower ornamental kale was rare since its low rate of embryogenesis. It is proved that histone acetylation might affect the gene expression in microspores and led to the transformation of microspores from pollen development pathway to embryogenesis. In this paper, microspores, derived from three varieties of cut flower ornamental kale, Crane Bicolor (CB), Crane Pink (CP) and Crane Feather Queen (CFQ), were treated with histone deacetylation inhibitor (HDACI) trichostatin A (TSA). Results revealed that the appropriate concentration of TSA was 10 nM for CB with obtaining 5.39 embryos per bud, while for CP and CFQ was 5 nM with acquiring 10.89 and 16.99 embryos per bud, respectively. TSA treatment also reduced the embryonic mortality, of which 10 nM TSA treatments CB was the optimal and the embryonic mortality decreased to 25.01%. The double haploid (DH) proportion of regenerated plants reached 37.3%. These results contribute to improving the technology for IMC in cut flower ornamental kale.https://www.mdpi.com/2311-7524/8/9/790embryo inductionisolated microspore cultureornamental kaleTSA |
| spellingShingle | Chuanhong Liu Gengxing Song Yonghui Zhao Bing Fang Zhiyong Liu Jie Ren Hui Feng Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (<i>Brassica oleracea</i> var. <i>acephala</i>) Horticulturae embryo induction isolated microspore culture ornamental kale TSA |
| title | Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (<i>Brassica oleracea</i> var. <i>acephala</i>) |
| title_full | Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (<i>Brassica oleracea</i> var. <i>acephala</i>) |
| title_fullStr | Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (<i>Brassica oleracea</i> var. <i>acephala</i>) |
| title_full_unstemmed | Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (<i>Brassica oleracea</i> var. <i>acephala</i>) |
| title_short | Trichostatin A Induced Microspore Embryogenesis and Promoted Plantlet Regeneration in Ornamental Kale (<i>Brassica oleracea</i> var. <i>acephala</i>) |
| title_sort | trichostatin a induced microspore embryogenesis and promoted plantlet regeneration in ornamental kale i brassica oleracea i var i acephala i |
| topic | embryo induction isolated microspore culture ornamental kale TSA |
| url | https://www.mdpi.com/2311-7524/8/9/790 |
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