GINGIVAL FIBROBLAST MICROVESICLES MODULATE PRO-B CELLS APOPTOSIS IN SPECIFIC EXPERIMENTAL CONDITIONS
Aim of the study The fast development of the tumor mass in acute lymphoblastic leukemia is dependent not only on the rate of cell proliferation, but also on the rate of apoptosis, with NR4A1 governing both tumor cell survival and death. Material and methods Treatment with 10 µm Cytosporone B was uti...
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| Format: | Article |
| Language: | English |
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Romanian Society of Oral Rehabilitation
2023-10-01
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| Series: | Romanian Journal of Oral Rehabilitation |
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| Online Access: | https://rjor.ro/gingival-fibroblast-microvesicles-modulate-pro-b-cells-apoptosis-in-specific-experimental-conditions/ |
| _version_ | 1797663368736145408 |
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| author | Carmen (Amititeloaie) Gentimir Gabriela Geleţu Andreea (Tibeică) Iordan Marcel Costuleanu |
| author_facet | Carmen (Amititeloaie) Gentimir Gabriela Geleţu Andreea (Tibeică) Iordan Marcel Costuleanu |
| author_sort | Carmen (Amititeloaie) Gentimir |
| collection | DOAJ |
| description | Aim of the study The fast development of the tumor mass in acute lymphoblastic leukemia is dependent not only on the rate of cell proliferation, but also on the rate of apoptosis, with NR4A1 governing both tumor cell survival and death. Material and methods Treatment with 10 µm Cytosporone B was utilized to induce pro-B cell apoptosis, with the latter being delivered in the culture medium for 48 hours. The active chemical reagents we used for our detailed experiments and protocols were as follows: 1 µm IAXO-102 (a TLR4 antagonist); 1 µm INH14 (TLR2 functional inhibitor); and 1 µm IL-7 (a promotor of pro-B transformation into pre-B cells). Results The real and distinguishable most important effects of increasing apoptosis of pro-B lymphocytes, induced by Cytosporone B when using microvesicles expelled by gingival fibroblasts, were linked to the following treatments, in reducing order: IAXO-102, IL-7, and INH14. Conclusions TLR4 and TLR2 inhibition, as well as IL-7 stimulation, may increase the apoptotic degree generated by Cytosporone B activation of NUR77/NR4A1 orphan receptors, as well as the presence of microvesicles released by gingival fibroblasts. |
| first_indexed | 2024-03-11T19:13:29Z |
| format | Article |
| id | doaj.art-d08fd2ffdc4f49d0acf48ab4923734e3 |
| institution | Directory Open Access Journal |
| issn | 2066-7000 2601-4661 |
| language | English |
| last_indexed | 2024-03-11T19:13:29Z |
| publishDate | 2023-10-01 |
| publisher | Romanian Society of Oral Rehabilitation |
| record_format | Article |
| series | Romanian Journal of Oral Rehabilitation |
| spelling | doaj.art-d08fd2ffdc4f49d0acf48ab4923734e32023-10-09T10:26:31ZengRomanian Society of Oral RehabilitationRomanian Journal of Oral Rehabilitation2066-70002601-46612023-10-01153239244GINGIVAL FIBROBLAST MICROVESICLES MODULATE PRO-B CELLS APOPTOSIS IN SPECIFIC EXPERIMENTAL CONDITIONSCarmen (Amititeloaie) Gentimir0Gabriela Geleţu1Andreea (Tibeică) Iordan2Marcel Costuleanu3Faculty of Dental Medicine, “Grigore T. Popa" University of Medicine and Pharmacy - Iași, RomaniaFaculty of Dental Medicine, “Grigore T. Popa" University of Medicine and Pharmacy - Iași, RomaniaFaculty of Dental Medicine, “Grigore T. Popa" University of Medicine and Pharmacy - Iași, RomaniaFaculty of Dental Medicine, “Grigore T. Popa" University of Medicine and Pharmacy - Iași, RomaniaAim of the study The fast development of the tumor mass in acute lymphoblastic leukemia is dependent not only on the rate of cell proliferation, but also on the rate of apoptosis, with NR4A1 governing both tumor cell survival and death. Material and methods Treatment with 10 µm Cytosporone B was utilized to induce pro-B cell apoptosis, with the latter being delivered in the culture medium for 48 hours. The active chemical reagents we used for our detailed experiments and protocols were as follows: 1 µm IAXO-102 (a TLR4 antagonist); 1 µm INH14 (TLR2 functional inhibitor); and 1 µm IL-7 (a promotor of pro-B transformation into pre-B cells). Results The real and distinguishable most important effects of increasing apoptosis of pro-B lymphocytes, induced by Cytosporone B when using microvesicles expelled by gingival fibroblasts, were linked to the following treatments, in reducing order: IAXO-102, IL-7, and INH14. Conclusions TLR4 and TLR2 inhibition, as well as IL-7 stimulation, may increase the apoptotic degree generated by Cytosporone B activation of NUR77/NR4A1 orphan receptors, as well as the presence of microvesicles released by gingival fibroblasts.https://rjor.ro/gingival-fibroblast-microvesicles-modulate-pro-b-cells-apoptosis-in-specific-experimental-conditions/pro-b lymphocyteapoptosistlr4tlr2il-7fibroblastmicrovesicle |
| spellingShingle | Carmen (Amititeloaie) Gentimir Gabriela Geleţu Andreea (Tibeică) Iordan Marcel Costuleanu GINGIVAL FIBROBLAST MICROVESICLES MODULATE PRO-B CELLS APOPTOSIS IN SPECIFIC EXPERIMENTAL CONDITIONS Romanian Journal of Oral Rehabilitation pro-b lymphocyte apoptosis tlr4 tlr2 il-7 fibroblast microvesicle |
| title | GINGIVAL FIBROBLAST MICROVESICLES MODULATE PRO-B CELLS APOPTOSIS IN SPECIFIC EXPERIMENTAL CONDITIONS |
| title_full | GINGIVAL FIBROBLAST MICROVESICLES MODULATE PRO-B CELLS APOPTOSIS IN SPECIFIC EXPERIMENTAL CONDITIONS |
| title_fullStr | GINGIVAL FIBROBLAST MICROVESICLES MODULATE PRO-B CELLS APOPTOSIS IN SPECIFIC EXPERIMENTAL CONDITIONS |
| title_full_unstemmed | GINGIVAL FIBROBLAST MICROVESICLES MODULATE PRO-B CELLS APOPTOSIS IN SPECIFIC EXPERIMENTAL CONDITIONS |
| title_short | GINGIVAL FIBROBLAST MICROVESICLES MODULATE PRO-B CELLS APOPTOSIS IN SPECIFIC EXPERIMENTAL CONDITIONS |
| title_sort | gingival fibroblast microvesicles modulate pro b cells apoptosis in specific experimental conditions |
| topic | pro-b lymphocyte apoptosis tlr4 tlr2 il-7 fibroblast microvesicle |
| url | https://rjor.ro/gingival-fibroblast-microvesicles-modulate-pro-b-cells-apoptosis-in-specific-experimental-conditions/ |
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