Regulation of cellular redox homeostasis in Arabidopsis thaliana seedling by atmospheric pressure cold plasma-generated reactive oxygen/nitrogen species
Atmospheric pressure cold plasma (APCP) holds great potential as an efficient, economical and eco-friendly approach for improving crop production. Although APCP-induced plant growth promotion is undisputedly attributed to the reactive oxygen and nitrogen species (RONS), how these RONS regulate the i...
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| Format: | Article |
| Sprog: | English |
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Elsevier
2022-07-01
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| Serier: | Ecotoxicology and Environmental Safety |
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| Online adgang: | http://www.sciencedirect.com/science/article/pii/S0147651322005437 |
| _version_ | 1828218885297405952 |
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| author | Dongjie Cui Yue Yin Hao Sun Xiaojie Wang Jie Zhuang Lin Wang Ruonan Ma Zhen Jiao |
| author_facet | Dongjie Cui Yue Yin Hao Sun Xiaojie Wang Jie Zhuang Lin Wang Ruonan Ma Zhen Jiao |
| author_sort | Dongjie Cui |
| collection | DOAJ |
| description | Atmospheric pressure cold plasma (APCP) holds great potential as an efficient, economical and eco-friendly approach for improving crop production. Although APCP-induced plant growth promotion is undisputedly attributed to the reactive oxygen and nitrogen species (RONS), how these RONS regulate the intracellular redox state and plant growth is still largely unknown. This study systematically investigates the regulation mechanism of APCP-generated RONS on intracellular redox homeostasis in Arabidopsis thaliana seedling by measuring the RONS compositions in APCP-treated solutions and intracellular RONS and antioxidants in Arabidopsis seedlings. The results show that APCP exhibited a dual effect (stimulation or inhibition) on Arabidopsis seedling growth dependent on the treatment time. APCP-generated RONS in liquids increased in a time-dependent manner, leading to an increase of conductivity and oxidation reduction potential (ORP) and decrease of pH. APCP caused an enrichment of intracellular RONS and most of them increased with APCP treatment time. Meanwhile, APCP treatment accelerated malondialdehyde (MDA) generation, and the level of intracellular antioxidants were enhanced by low-dose APCP treatment while decreased at high doses. The results of correlation analysis showed that the extracellular RONS produced by APCP were positively correlated with the intracellular RONS and negatively correlated with the antioxidants. These results demonstrate that the improved antioxidant capacity induced by moderate APCP-generated RONS plays an important role in the growth promotion of Arabidopsis seedlings, which may be a promising alternative for fertilizers in agricultural production. |
| first_indexed | 2024-04-12T16:09:20Z |
| format | Article |
| id | doaj.art-d0908546f67b4e02819e38e143b18afa |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-12T16:09:20Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-d0908546f67b4e02819e38e143b18afa2022-12-22T03:25:57ZengElsevierEcotoxicology and Environmental Safety0147-65132022-07-01240113703Regulation of cellular redox homeostasis in Arabidopsis thaliana seedling by atmospheric pressure cold plasma-generated reactive oxygen/nitrogen speciesDongjie Cui0Yue Yin1Hao Sun2Xiaojie Wang3Jie Zhuang4Lin Wang5Ruonan Ma6Zhen Jiao7Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, People’s Republic of China; Henan Key Laboratory of Ion-Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, People’s Republic of ChinaHenan Key Laboratory of Ion-Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, People’s Republic of ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, People’s Republic of ChinaSchool of Life Sciences and Basic Medicine, Xinxiang University, Xinxiang 453003, People’s Republic of ChinaSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, People’s Republic of ChinaCollege of Biology and Food Engineering, Anyang Institute of Technology, Anyang 455000, People’s Republic of ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, People’s Republic of China; Henan Key Laboratory of Ion-Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, People’s Republic of China; Corresponding authors at: Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, People’s Republic of China.Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, People’s Republic of China; Henan Key Laboratory of Ion-Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, People’s Republic of China; Corresponding authors at: Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, People’s Republic of China.Atmospheric pressure cold plasma (APCP) holds great potential as an efficient, economical and eco-friendly approach for improving crop production. Although APCP-induced plant growth promotion is undisputedly attributed to the reactive oxygen and nitrogen species (RONS), how these RONS regulate the intracellular redox state and plant growth is still largely unknown. This study systematically investigates the regulation mechanism of APCP-generated RONS on intracellular redox homeostasis in Arabidopsis thaliana seedling by measuring the RONS compositions in APCP-treated solutions and intracellular RONS and antioxidants in Arabidopsis seedlings. The results show that APCP exhibited a dual effect (stimulation or inhibition) on Arabidopsis seedling growth dependent on the treatment time. APCP-generated RONS in liquids increased in a time-dependent manner, leading to an increase of conductivity and oxidation reduction potential (ORP) and decrease of pH. APCP caused an enrichment of intracellular RONS and most of them increased with APCP treatment time. Meanwhile, APCP treatment accelerated malondialdehyde (MDA) generation, and the level of intracellular antioxidants were enhanced by low-dose APCP treatment while decreased at high doses. The results of correlation analysis showed that the extracellular RONS produced by APCP were positively correlated with the intracellular RONS and negatively correlated with the antioxidants. These results demonstrate that the improved antioxidant capacity induced by moderate APCP-generated RONS plays an important role in the growth promotion of Arabidopsis seedlings, which may be a promising alternative for fertilizers in agricultural production.http://www.sciencedirect.com/science/article/pii/S0147651322005437Atmospheric pressure cold plasmaArabidopsis thalianaSeedling growth promotionReactive oxygen/nitrogen speciesAntioxidant capacityOxidative stress |
| spellingShingle | Dongjie Cui Yue Yin Hao Sun Xiaojie Wang Jie Zhuang Lin Wang Ruonan Ma Zhen Jiao Regulation of cellular redox homeostasis in Arabidopsis thaliana seedling by atmospheric pressure cold plasma-generated reactive oxygen/nitrogen species Ecotoxicology and Environmental Safety Atmospheric pressure cold plasma Arabidopsis thaliana Seedling growth promotion Reactive oxygen/nitrogen species Antioxidant capacity Oxidative stress |
| title | Regulation of cellular redox homeostasis in Arabidopsis thaliana seedling by atmospheric pressure cold plasma-generated reactive oxygen/nitrogen species |
| title_full | Regulation of cellular redox homeostasis in Arabidopsis thaliana seedling by atmospheric pressure cold plasma-generated reactive oxygen/nitrogen species |
| title_fullStr | Regulation of cellular redox homeostasis in Arabidopsis thaliana seedling by atmospheric pressure cold plasma-generated reactive oxygen/nitrogen species |
| title_full_unstemmed | Regulation of cellular redox homeostasis in Arabidopsis thaliana seedling by atmospheric pressure cold plasma-generated reactive oxygen/nitrogen species |
| title_short | Regulation of cellular redox homeostasis in Arabidopsis thaliana seedling by atmospheric pressure cold plasma-generated reactive oxygen/nitrogen species |
| title_sort | regulation of cellular redox homeostasis in arabidopsis thaliana seedling by atmospheric pressure cold plasma generated reactive oxygen nitrogen species |
| topic | Atmospheric pressure cold plasma Arabidopsis thaliana Seedling growth promotion Reactive oxygen/nitrogen species Antioxidant capacity Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322005437 |
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