Increasing vineyard sustainability: innovating a targeted chitosan-derived biocontrol solution to induce grapevine resistance against downy and powdery mildews
The European Green Deal aims to reduce the pesticide use, notably by developing biocontrol products to protect crops from diseases. Indeed, the use of significant amounts of chemicals negatively impact the environment such as soil microbial biodiversity or groundwater quality, and human health. Grap...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-02-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1360254/full |
| _version_ | 1797322449947197440 |
|---|---|
| author | Daphnée Brulé Marie-Claire Héloir Thibault Roudaire Jérémy Villette Silvère Bonnet Yoann Pascal Benoît Darblade Philippe Crozier Philippe Hugueney Véronique Coma Benoit Poinssot |
| author_facet | Daphnée Brulé Marie-Claire Héloir Thibault Roudaire Jérémy Villette Silvère Bonnet Yoann Pascal Benoît Darblade Philippe Crozier Philippe Hugueney Véronique Coma Benoit Poinssot |
| author_sort | Daphnée Brulé |
| collection | DOAJ |
| description | The European Green Deal aims to reduce the pesticide use, notably by developing biocontrol products to protect crops from diseases. Indeed, the use of significant amounts of chemicals negatively impact the environment such as soil microbial biodiversity or groundwater quality, and human health. Grapevine (Vitis vinifera) was selected as one of the first targeted crop due to its economic importance and its dependence on fungicides to control the main damaging diseases worldwide: grey mold, downy and powdery mildews. Chitosan, a biopolymer extracted from crustacean exoskeletons, has been used as a biocontrol agent in many plant species, including grapevine, against a variety of cryptogamic diseases such as downy mildew (Plasmopara viticola), powdery mildew (Erysiphe necator) and grey mold (Botrytis cinerea). However, the precise molecular mechanisms underlying its mode of action remain unclear: is it a direct biopesticide effect or an indirect elicitation activity, or both? In this study, we investigated six chitosans with diverse degrees of polymerization (DP) ranging from low to high DP (12, 25, 33, 44, 100, and 470). We scrutinized their biological activities by evaluating both their antifungal properties and their abilities to induce grapevine immune responses. To investigate their elicitor activity, we analyzed their ability to induce MAPKs phosphorylation, the activation of defense genes and metabolite changes in grapevine. Our results indicate that the chitosans with a low DP are more effective in inducing grapevine defenses and possess the strongest biopesticide effect against B. cinerea and P. viticola. We identified chitosan with DP12 as the most efficient resistance inducer. Then, chitosan DP12 has been tested against downy and powdery mildews in the vineyard trials performed during the last three years. Results obtained indicated that a chitosan-based biocontrol product could be sufficiently efficient when the amount of pathogen inoculum is quite low and could be combined with only two fungicide treatments during whole season programs to obtain a good protection efficiency. On the whole, a chitosan-based biocontrol product could become an interesting alternative to meet the chemicals reduction targeted in sustainable viticulture. |
| first_indexed | 2024-03-08T05:13:33Z |
| format | Article |
| id | doaj.art-d09169279a8d4d18bb9f1f36e30b9f38 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T05:13:33Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d09169279a8d4d18bb9f1f36e30b9f382024-02-07T05:23:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-02-011510.3389/fpls.2024.13602541360254Increasing vineyard sustainability: innovating a targeted chitosan-derived biocontrol solution to induce grapevine resistance against downy and powdery mildewsDaphnée Brulé0Marie-Claire Héloir1Thibault Roudaire2Jérémy Villette3Silvère Bonnet4Yoann Pascal5Benoît Darblade6Philippe Crozier7Philippe Hugueney8Véronique Coma9Benoit Poinssot10UMR Agroécologie, INRAE, Institut Agro Dijon, Université de Bourgogne, Dijon, FranceUMR Agroécologie, INRAE, Institut Agro Dijon, Université de Bourgogne, Dijon, FranceUMR Agroécologie, INRAE, Institut Agro Dijon, Université de Bourgogne, Dijon, FranceUMR Agroécologie, INRAE, Institut Agro Dijon, Université de Bourgogne, Dijon, FranceElicityl, Crolles, FranceElicityl, Crolles, FranceElicityl, Crolles, FrancePhyteurop, Paris, FranceUMR-A 1131 Santé de la Vigne et Qualité du Vin (SVQV), Université de Strasbourg, INRAE, Colmar, FranceLaboratoire de Chimie des Polymères Organiques, Université de Bordeaux, CNRS, Bordeaux INP, UMR 5629, Pessac, FranceUMR Agroécologie, INRAE, Institut Agro Dijon, Université de Bourgogne, Dijon, FranceThe European Green Deal aims to reduce the pesticide use, notably by developing biocontrol products to protect crops from diseases. Indeed, the use of significant amounts of chemicals negatively impact the environment such as soil microbial biodiversity or groundwater quality, and human health. Grapevine (Vitis vinifera) was selected as one of the first targeted crop due to its economic importance and its dependence on fungicides to control the main damaging diseases worldwide: grey mold, downy and powdery mildews. Chitosan, a biopolymer extracted from crustacean exoskeletons, has been used as a biocontrol agent in many plant species, including grapevine, against a variety of cryptogamic diseases such as downy mildew (Plasmopara viticola), powdery mildew (Erysiphe necator) and grey mold (Botrytis cinerea). However, the precise molecular mechanisms underlying its mode of action remain unclear: is it a direct biopesticide effect or an indirect elicitation activity, or both? In this study, we investigated six chitosans with diverse degrees of polymerization (DP) ranging from low to high DP (12, 25, 33, 44, 100, and 470). We scrutinized their biological activities by evaluating both their antifungal properties and their abilities to induce grapevine immune responses. To investigate their elicitor activity, we analyzed their ability to induce MAPKs phosphorylation, the activation of defense genes and metabolite changes in grapevine. Our results indicate that the chitosans with a low DP are more effective in inducing grapevine defenses and possess the strongest biopesticide effect against B. cinerea and P. viticola. We identified chitosan with DP12 as the most efficient resistance inducer. Then, chitosan DP12 has been tested against downy and powdery mildews in the vineyard trials performed during the last three years. Results obtained indicated that a chitosan-based biocontrol product could be sufficiently efficient when the amount of pathogen inoculum is quite low and could be combined with only two fungicide treatments during whole season programs to obtain a good protection efficiency. On the whole, a chitosan-based biocontrol product could become an interesting alternative to meet the chemicals reduction targeted in sustainable viticulture.https://www.frontiersin.org/articles/10.3389/fpls.2024.1360254/fullVitis viniferainduced resistancebiocontrol productchito-oligosaccharideschitosandegree of polymerization |
| spellingShingle | Daphnée Brulé Marie-Claire Héloir Thibault Roudaire Jérémy Villette Silvère Bonnet Yoann Pascal Benoît Darblade Philippe Crozier Philippe Hugueney Véronique Coma Benoit Poinssot Increasing vineyard sustainability: innovating a targeted chitosan-derived biocontrol solution to induce grapevine resistance against downy and powdery mildews Frontiers in Plant Science Vitis vinifera induced resistance biocontrol product chito-oligosaccharides chitosan degree of polymerization |
| title | Increasing vineyard sustainability: innovating a targeted chitosan-derived biocontrol solution to induce grapevine resistance against downy and powdery mildews |
| title_full | Increasing vineyard sustainability: innovating a targeted chitosan-derived biocontrol solution to induce grapevine resistance against downy and powdery mildews |
| title_fullStr | Increasing vineyard sustainability: innovating a targeted chitosan-derived biocontrol solution to induce grapevine resistance against downy and powdery mildews |
| title_full_unstemmed | Increasing vineyard sustainability: innovating a targeted chitosan-derived biocontrol solution to induce grapevine resistance against downy and powdery mildews |
| title_short | Increasing vineyard sustainability: innovating a targeted chitosan-derived biocontrol solution to induce grapevine resistance against downy and powdery mildews |
| title_sort | increasing vineyard sustainability innovating a targeted chitosan derived biocontrol solution to induce grapevine resistance against downy and powdery mildews |
| topic | Vitis vinifera induced resistance biocontrol product chito-oligosaccharides chitosan degree of polymerization |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1360254/full |
| work_keys_str_mv | AT daphneebrule increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT marieclaireheloir increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT thibaultroudaire increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT jeremyvillette increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT silverebonnet increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT yoannpascal increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT benoitdarblade increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT philippecrozier increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT philippehugueney increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT veroniquecoma increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews AT benoitpoinssot increasingvineyardsustainabilityinnovatingatargetedchitosanderivedbiocontrolsolutiontoinducegrapevineresistanceagainstdownyandpowderymildews |