Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects
IntroductionIn recent years, several studies have evidenced the importance of the microbiome to host physiology as metabolism regulator, along with its potential role in triggering various diseases. In this study, we analyzed the gut microbiota in hypercholesterolemic (cases) and normocholesterolemi...
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Frontiers Media S.A.
2022-11-01
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Series: | Frontiers in Cellular and Infection Microbiology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.943609/full |
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author | Cristian Morales Cristian Morales Gabriel Rojas Camilo Rebolledo Marcelo Rojas-Herrera Marcelo Rojas-Herrera Raúl Arias-Carrasco Sara Cuadros-Orellana Vinicius Maracaja-Coutinho Kathleen Saavedra Pamela Leal Fernando Lanas Luis A. Salazar Nicolás Saavedra |
author_facet | Cristian Morales Cristian Morales Gabriel Rojas Camilo Rebolledo Marcelo Rojas-Herrera Marcelo Rojas-Herrera Raúl Arias-Carrasco Sara Cuadros-Orellana Vinicius Maracaja-Coutinho Kathleen Saavedra Pamela Leal Fernando Lanas Luis A. Salazar Nicolás Saavedra |
author_sort | Cristian Morales |
collection | DOAJ |
description | IntroductionIn recent years, several studies have evidenced the importance of the microbiome to host physiology as metabolism regulator, along with its potential role in triggering various diseases. In this study, we analyzed the gut microbiota in hypercholesterolemic (cases) and normocholesterolemic (controls) individuals to identify characteristic microbial signature for each condition.MethodsStool samples were obtained from 57 adult volunteers (27 hypercholesterolemic and 30 controls). The taxonomic profiling of microbial communities was performed using high-throughput sequencing of 16S rRNA V3-V4 amplicons, followed by data analysis using Quantitative Insights Into Microbial Ecology 2 (QIIME2) and linear discriminant analysis (LDA) effect size (LEfSe).ResultsSignificant differences were observed in weight, height, body mass index (BMI) and serum levels of triglycerides, total cholesterol and low-density lipoprotein cholesterol (LDL-C) between the groups (p<0.05). LEfSe showed differentially abundant prokaryotic taxa (α=0.05, LDA score > 2.0) in the group of hypercholesterolemic individuals (Methanosphaera, Rothia, Chromatiales, Clostridiales, Bacillaceae and Coriobacteriaceae) and controls (Faecalibacterium, Victivallis and Selenomonas) at various taxonomic levels. In addition, through the application of Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2 (PICRUSt2), the predominance of pathways related to biosynthesis in hypercholesterolemic patients was established, compared to controls in which degradation pathways were predominant. Finally, in the analysis of co-occurrence networks, it was possible to identify associations between the microorganisms present in both studied groups.ConclusionOur results point out to unique microbial signatures, which likely play a role on the cholesterol metabolism in the studied population. |
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spelling | doaj.art-d091bb7edd4c48e3b20bad139911a86f2022-12-22T04:16:44ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-11-011210.3389/fcimb.2022.943609943609Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjectsCristian Morales0Cristian Morales1Gabriel Rojas2Camilo Rebolledo3Marcelo Rojas-Herrera4Marcelo Rojas-Herrera5Raúl Arias-Carrasco6Sara Cuadros-Orellana7Vinicius Maracaja-Coutinho8Kathleen Saavedra9Pamela Leal10Fernando Lanas11Luis A. Salazar12Nicolás Saavedra13Centro de Biología Molecular y Farmacogenética, Núcleo Científico-Tecnológico en Biorecursos BIOREN, Universidad de La Frontera, Temuco, ChileTecnología Médica, Facultad de Salud, Universidad Santo Tomás, Temuco, ChileCentro de Biología Molecular y Farmacogenética, Núcleo Científico-Tecnológico en Biorecursos BIOREN, Universidad de La Frontera, Temuco, ChileCentro de Biología Molecular y Farmacogenética, Núcleo Científico-Tecnológico en Biorecursos BIOREN, Universidad de La Frontera, Temuco, ChileCentro de Genética y Genómica, Facultad de Medicina, Universidad del Desarrollo, Santiago, ChileSubdepartamento de Genética Molecular, Instituto de Salud Pública de Chile, Santiago, ChilePrograma Institucional de Fomento a la Investigación, Desarrollo e Innovación, Universidad Tecnológica Metropolitana, Santiago, ChileCentro de Biotecnología de los Recursos Naturales, Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Talca, ChileAdvanced Center for Chronic Diseases - ACCDiS, Facultad de Química y Ciencias Farmacéuticas, Universidad de Chile, Santiago, ChileCentro de Biología Molecular y Farmacogenética, Núcleo Científico-Tecnológico en Biorecursos BIOREN, Universidad de La Frontera, Temuco, ChileDepartamento de Ciencias Agronómicas y Recursos Naturales, Facultad de Ciencias Agropecuarias y Medioambiente, Temuco, ChileDepartamento de Medicina Interna, Facultad de Medicina, Universidad de La Frontera, Temuco, ChileCentro de Biología Molecular y Farmacogenética, Núcleo Científico-Tecnológico en Biorecursos BIOREN, Universidad de La Frontera, Temuco, ChileCentro de Biología Molecular y Farmacogenética, Núcleo Científico-Tecnológico en Biorecursos BIOREN, Universidad de La Frontera, Temuco, ChileIntroductionIn recent years, several studies have evidenced the importance of the microbiome to host physiology as metabolism regulator, along with its potential role in triggering various diseases. In this study, we analyzed the gut microbiota in hypercholesterolemic (cases) and normocholesterolemic (controls) individuals to identify characteristic microbial signature for each condition.MethodsStool samples were obtained from 57 adult volunteers (27 hypercholesterolemic and 30 controls). The taxonomic profiling of microbial communities was performed using high-throughput sequencing of 16S rRNA V3-V4 amplicons, followed by data analysis using Quantitative Insights Into Microbial Ecology 2 (QIIME2) and linear discriminant analysis (LDA) effect size (LEfSe).ResultsSignificant differences were observed in weight, height, body mass index (BMI) and serum levels of triglycerides, total cholesterol and low-density lipoprotein cholesterol (LDL-C) between the groups (p<0.05). LEfSe showed differentially abundant prokaryotic taxa (α=0.05, LDA score > 2.0) in the group of hypercholesterolemic individuals (Methanosphaera, Rothia, Chromatiales, Clostridiales, Bacillaceae and Coriobacteriaceae) and controls (Faecalibacterium, Victivallis and Selenomonas) at various taxonomic levels. In addition, through the application of Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2 (PICRUSt2), the predominance of pathways related to biosynthesis in hypercholesterolemic patients was established, compared to controls in which degradation pathways were predominant. Finally, in the analysis of co-occurrence networks, it was possible to identify associations between the microorganisms present in both studied groups.ConclusionOur results point out to unique microbial signatures, which likely play a role on the cholesterol metabolism in the studied population.https://www.frontiersin.org/articles/10.3389/fcimb.2022.943609/fullgut microbiotaLDL cholesterolLEfSe analysismicrobial signature16S rRNA sequencing |
spellingShingle | Cristian Morales Cristian Morales Gabriel Rojas Camilo Rebolledo Marcelo Rojas-Herrera Marcelo Rojas-Herrera Raúl Arias-Carrasco Sara Cuadros-Orellana Vinicius Maracaja-Coutinho Kathleen Saavedra Pamela Leal Fernando Lanas Luis A. Salazar Nicolás Saavedra Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects Frontiers in Cellular and Infection Microbiology gut microbiota LDL cholesterol LEfSe analysis microbial signature 16S rRNA sequencing |
title | Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects |
title_full | Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects |
title_fullStr | Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects |
title_full_unstemmed | Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects |
title_short | Characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects |
title_sort | characterization of microbial communities from gut microbiota of hypercholesterolemic and control subjects |
topic | gut microbiota LDL cholesterol LEfSe analysis microbial signature 16S rRNA sequencing |
url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.943609/full |
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