New Cell Adhesion Molecules in Human Ischemic Cardiomyopathy. PCDHGA3 Implications in Decreased Stroke Volume and Ventricular Dysfunction.
Intercalated disks are unique structures in cardiac tissue, in which adherens junctions, desmosomes, and GAP junctions co-localize, thereby facilitating cardiac muscle contraction and function. Protocadherins are involved in these junctions; however, their role in heart physiology is poorly understo...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4966940?pdf=render |
| _version_ | 1819042085709283328 |
|---|---|
| author | Ana Ortega Carolina Gil-Cayuela Estefanía Tarazón María García-Manzanares José Anastasio Montero Juan Cinca Manuel Portolés Miguel Rivera Esther Roselló-Lletí |
| author_facet | Ana Ortega Carolina Gil-Cayuela Estefanía Tarazón María García-Manzanares José Anastasio Montero Juan Cinca Manuel Portolés Miguel Rivera Esther Roselló-Lletí |
| author_sort | Ana Ortega |
| collection | DOAJ |
| description | Intercalated disks are unique structures in cardiac tissue, in which adherens junctions, desmosomes, and GAP junctions co-localize, thereby facilitating cardiac muscle contraction and function. Protocadherins are involved in these junctions; however, their role in heart physiology is poorly understood. We aimed to analyze the transcriptomic profile of adhesion molecules in patients with ischemic cardiomyopathy (ICM) and relate the changes uncovered with the hemodynamic alterations and functional depression observed in these patients.Twenty-three left ventricular tissue samples from patients diagnosed with ICM (n = 13) undergoing heart transplantation and control donors (CNT, n = 10) were analyzed using RNA sequencing. Forty-two cell adhesion genes involved in cellular junctions were differentially expressed in ICM myocardium. Notably, the levels of protocadherin PCDHGA3 were related with the stroke volume (r = -0.826, P = 0.003), ejection fraction (r = -0.793, P = 0.004) and left ventricular end systolic and diastolic diameters (r = 0.867, P = 0.001; r = 0.781, P = 0.005, respectively).Our results support the importance of intercalated disks molecular alterations, closely involved in the contractile function, highlighting its crucial significance and showing gene expression changes not previously described. Specifically, altered PCDHGA3 gene expression was strongly associated with reduced stroke volume and ventricular dysfunction in ICM, suggesting a relevant role in hemodynamic perturbations and cardiac performance for this unexplored protocadherin. |
| first_indexed | 2024-12-21T09:35:17Z |
| format | Article |
| id | doaj.art-d091dc5a97834963b62d66edff93abca |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T09:35:17Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d091dc5a97834963b62d66edff93abca2022-12-21T19:08:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e016016810.1371/journal.pone.0160168New Cell Adhesion Molecules in Human Ischemic Cardiomyopathy. PCDHGA3 Implications in Decreased Stroke Volume and Ventricular Dysfunction.Ana OrtegaCarolina Gil-CayuelaEstefanía TarazónMaría García-ManzanaresJosé Anastasio MonteroJuan CincaManuel PortolésMiguel RiveraEsther Roselló-LletíIntercalated disks are unique structures in cardiac tissue, in which adherens junctions, desmosomes, and GAP junctions co-localize, thereby facilitating cardiac muscle contraction and function. Protocadherins are involved in these junctions; however, their role in heart physiology is poorly understood. We aimed to analyze the transcriptomic profile of adhesion molecules in patients with ischemic cardiomyopathy (ICM) and relate the changes uncovered with the hemodynamic alterations and functional depression observed in these patients.Twenty-three left ventricular tissue samples from patients diagnosed with ICM (n = 13) undergoing heart transplantation and control donors (CNT, n = 10) were analyzed using RNA sequencing. Forty-two cell adhesion genes involved in cellular junctions were differentially expressed in ICM myocardium. Notably, the levels of protocadherin PCDHGA3 were related with the stroke volume (r = -0.826, P = 0.003), ejection fraction (r = -0.793, P = 0.004) and left ventricular end systolic and diastolic diameters (r = 0.867, P = 0.001; r = 0.781, P = 0.005, respectively).Our results support the importance of intercalated disks molecular alterations, closely involved in the contractile function, highlighting its crucial significance and showing gene expression changes not previously described. Specifically, altered PCDHGA3 gene expression was strongly associated with reduced stroke volume and ventricular dysfunction in ICM, suggesting a relevant role in hemodynamic perturbations and cardiac performance for this unexplored protocadherin.http://europepmc.org/articles/PMC4966940?pdf=render |
| spellingShingle | Ana Ortega Carolina Gil-Cayuela Estefanía Tarazón María García-Manzanares José Anastasio Montero Juan Cinca Manuel Portolés Miguel Rivera Esther Roselló-Lletí New Cell Adhesion Molecules in Human Ischemic Cardiomyopathy. PCDHGA3 Implications in Decreased Stroke Volume and Ventricular Dysfunction. PLoS ONE |
| title | New Cell Adhesion Molecules in Human Ischemic Cardiomyopathy. PCDHGA3 Implications in Decreased Stroke Volume and Ventricular Dysfunction. |
| title_full | New Cell Adhesion Molecules in Human Ischemic Cardiomyopathy. PCDHGA3 Implications in Decreased Stroke Volume and Ventricular Dysfunction. |
| title_fullStr | New Cell Adhesion Molecules in Human Ischemic Cardiomyopathy. PCDHGA3 Implications in Decreased Stroke Volume and Ventricular Dysfunction. |
| title_full_unstemmed | New Cell Adhesion Molecules in Human Ischemic Cardiomyopathy. PCDHGA3 Implications in Decreased Stroke Volume and Ventricular Dysfunction. |
| title_short | New Cell Adhesion Molecules in Human Ischemic Cardiomyopathy. PCDHGA3 Implications in Decreased Stroke Volume and Ventricular Dysfunction. |
| title_sort | new cell adhesion molecules in human ischemic cardiomyopathy pcdhga3 implications in decreased stroke volume and ventricular dysfunction |
| url | http://europepmc.org/articles/PMC4966940?pdf=render |
| work_keys_str_mv | AT anaortega newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction AT carolinagilcayuela newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction AT estefaniatarazon newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction AT mariagarciamanzanares newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction AT joseanastasiomontero newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction AT juancinca newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction AT manuelportoles newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction AT miguelrivera newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction AT estherrosellolleti newcelladhesionmoleculesinhumanischemiccardiomyopathypcdhga3implicationsindecreasedstrokevolumeandventriculardysfunction |