Impact of Sacbrood Virus on Larval Microbiome of <i>Apis mellifera</i> and <i>Apis cerana</i>
In this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (<i>Apis mellifera</i>) death, producing a liquefied a larva sac, on the gut bacterial communities on two larval honeybee species, <i>Apis mellifera</i> and <i>Apis cerana</i&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/7/439 |
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| author | Rujipas Yongsawas Veeranan Chaimanee Jeffery S. Pettis Humberto Freire Boncristiani Junior Dawn Lopez Ammarin In-on Panuwan Chantawannakul Terd Disayathanoowat |
| author_facet | Rujipas Yongsawas Veeranan Chaimanee Jeffery S. Pettis Humberto Freire Boncristiani Junior Dawn Lopez Ammarin In-on Panuwan Chantawannakul Terd Disayathanoowat |
| author_sort | Rujipas Yongsawas |
| collection | DOAJ |
| description | In this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (<i>Apis mellifera</i>) death, producing a liquefied a larva sac, on the gut bacterial communities on two larval honeybee species, <i>Apis mellifera</i> and <i>Apis cerana</i>. SBV was added into a worker jelly food mixture and bee larvae were grafted into each of the treatment groups for 24 h before DNA/RNA extraction. Confirmation of SBV infection was achieved using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and visual symptomology. The 16S rDNA was sequenced by Illumina sequencing. The results showed the larvae were infected with SBV. The gut communities of infected <i>A. cerana</i> larvae exhibited a dramatic change compared with <i>A. mellifera</i>. In <i>A. mellifera</i> larvae, the Illumina sequencing revealed the proportion of <i>Gilliamella</i>, <i>Snodgrassella</i> and <i>Fructobacillus</i> was not significantly different, whereas in <i>A. cerana</i>, <i>Gilliamella</i> was significantly decreased (from 35.54% to 2.96%), however, with significant increase in <i>Snodgrassella</i> and <i>Fructobacillus</i>. The possibility of cross-infection should be further investigated. |
| first_indexed | 2024-03-10T18:30:59Z |
| format | Article |
| id | doaj.art-d0934906b7814abeb8b072d773e3fb3e |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T18:30:59Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-d0934906b7814abeb8b072d773e3fb3e2023-11-20T06:40:01ZengMDPI AGInsects2075-44502020-07-0111743910.3390/insects11070439Impact of Sacbrood Virus on Larval Microbiome of <i>Apis mellifera</i> and <i>Apis cerana</i>Rujipas Yongsawas0Veeranan Chaimanee1Jeffery S. Pettis2Humberto Freire Boncristiani Junior3Dawn Lopez4Ammarin In-on5Panuwan Chantawannakul6Terd Disayathanoowat7Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Agro-Industrial Biotechnology, Maejo University Phrae Campus, Rong Kwang, Phrae 54140, ThailandPettis and Associates LLC, Salisbury, MD 21801, USAHoney Bee Research and Extension Laboratory, Entomology and Nematology Department, University of Florida, Gainnesville, FL 32611, USABee Research Laboratory, USDA-ARS, Beltsville, MD 20705, USABioinformatics & Systems Biology Program, King Mongkut’s University of Technology Thonburi (Bang Khun Thian Campus), Bang Khun Thian, Bangkok 10150, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandIn this study, we examined the impact of Sacbrood virus (SBV), the cause of larval honeybee (<i>Apis mellifera</i>) death, producing a liquefied a larva sac, on the gut bacterial communities on two larval honeybee species, <i>Apis mellifera</i> and <i>Apis cerana</i>. SBV was added into a worker jelly food mixture and bee larvae were grafted into each of the treatment groups for 24 h before DNA/RNA extraction. Confirmation of SBV infection was achieved using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and visual symptomology. The 16S rDNA was sequenced by Illumina sequencing. The results showed the larvae were infected with SBV. The gut communities of infected <i>A. cerana</i> larvae exhibited a dramatic change compared with <i>A. mellifera</i>. In <i>A. mellifera</i> larvae, the Illumina sequencing revealed the proportion of <i>Gilliamella</i>, <i>Snodgrassella</i> and <i>Fructobacillus</i> was not significantly different, whereas in <i>A. cerana</i>, <i>Gilliamella</i> was significantly decreased (from 35.54% to 2.96%), however, with significant increase in <i>Snodgrassella</i> and <i>Fructobacillus</i>. The possibility of cross-infection should be further investigated.https://www.mdpi.com/2075-4450/11/7/439<i>Apis mellifera</i><i>Apis cerana</i>bee virusSacbrood viruscross-infectiongut microbiome |
| spellingShingle | Rujipas Yongsawas Veeranan Chaimanee Jeffery S. Pettis Humberto Freire Boncristiani Junior Dawn Lopez Ammarin In-on Panuwan Chantawannakul Terd Disayathanoowat Impact of Sacbrood Virus on Larval Microbiome of <i>Apis mellifera</i> and <i>Apis cerana</i> Insects <i>Apis mellifera</i> <i>Apis cerana</i> bee virus Sacbrood virus cross-infection gut microbiome |
| title | Impact of Sacbrood Virus on Larval Microbiome of <i>Apis mellifera</i> and <i>Apis cerana</i> |
| title_full | Impact of Sacbrood Virus on Larval Microbiome of <i>Apis mellifera</i> and <i>Apis cerana</i> |
| title_fullStr | Impact of Sacbrood Virus on Larval Microbiome of <i>Apis mellifera</i> and <i>Apis cerana</i> |
| title_full_unstemmed | Impact of Sacbrood Virus on Larval Microbiome of <i>Apis mellifera</i> and <i>Apis cerana</i> |
| title_short | Impact of Sacbrood Virus on Larval Microbiome of <i>Apis mellifera</i> and <i>Apis cerana</i> |
| title_sort | impact of sacbrood virus on larval microbiome of i apis mellifera i and i apis cerana i |
| topic | <i>Apis mellifera</i> <i>Apis cerana</i> bee virus Sacbrood virus cross-infection gut microbiome |
| url | https://www.mdpi.com/2075-4450/11/7/439 |
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