High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee
HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles. However, the underlying molecular mechanisms of the processes are poorly understood. In this study, we used the simple model organism honey bee to explore how different am...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/834 |
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| author | Xiaofei Wang Zhaopeng Zhong Xiangyin Chen Ziyun Hong Weimin Lin Xiaohuan Mu Xiaosong Hu Hao Zheng |
| author_facet | Xiaofei Wang Zhaopeng Zhong Xiangyin Chen Ziyun Hong Weimin Lin Xiaohuan Mu Xiaosong Hu Hao Zheng |
| author_sort | Xiaofei Wang |
| collection | DOAJ |
| description | HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles. However, the underlying molecular mechanisms of the processes are poorly understood. In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota. Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees. However, microbiota-free honey bees reared on high-fat diets did not significantly change their phenotypes. Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body. Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level. Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats. Moreover, a high-fat diet increased the relative abundance of <i>Gilliamella</i>, while the level of <i>Bartonella</i> was significantly decreased in palm oil groups. This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism. |
| first_indexed | 2024-03-09T04:38:30Z |
| format | Article |
| id | doaj.art-d0974e035e4a496c96db4c110dcae768 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:38:30Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d0974e035e4a496c96db4c110dcae7682023-12-03T13:25:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122283410.3390/ijms22020834High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey BeeXiaofei Wang0Zhaopeng Zhong1Xiangyin Chen2Ziyun Hong3Weimin Lin4Xiaohuan Mu5Xiaosong Hu6Hao Zheng7College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, ChinaHFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles. However, the underlying molecular mechanisms of the processes are poorly understood. In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota. Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees. However, microbiota-free honey bees reared on high-fat diets did not significantly change their phenotypes. Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body. Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level. Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats. Moreover, a high-fat diet increased the relative abundance of <i>Gilliamella</i>, while the level of <i>Bartonella</i> was significantly decreased in palm oil groups. This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.https://www.mdpi.com/1422-0067/22/2/834gut microbiotadietary fatfat accumulationlipid metabolismhoney bee |
| spellingShingle | Xiaofei Wang Zhaopeng Zhong Xiangyin Chen Ziyun Hong Weimin Lin Xiaohuan Mu Xiaosong Hu Hao Zheng High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee International Journal of Molecular Sciences gut microbiota dietary fat fat accumulation lipid metabolism honey bee |
| title | High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee |
| title_full | High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee |
| title_fullStr | High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee |
| title_full_unstemmed | High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee |
| title_short | High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee |
| title_sort | high fat diets with differential fatty acids induce obesity and perturb gut microbiota in honey bee |
| topic | gut microbiota dietary fat fat accumulation lipid metabolism honey bee |
| url | https://www.mdpi.com/1422-0067/22/2/834 |
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