Diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicity
We examined synergistic effects of inhibiting reactive oxygen species generated from the mitochondria and from nicotinamide adenine dinucleotide phosphate oxidase on neurotoxicity. Primary hippocampal neurons were exposed to amyloid β, and the cells were treated with diazoxide or/and diphenyleneiodo...
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| Format: | Article |
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IMR Press
2019-12-01
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| Series: | Journal of Integrative Neuroscience |
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https://jin.imrpress.com/fileup/1757-448X/PDF/1577791324315-850926386.pdf
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| _version_ | 1818231262675992576 |
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| author | Qingxi Fu Naiyong Gao Fengyuan Che Guangyin Xu Hongxing Han Quanping Su Guozhao Ma |
| author_facet | Qingxi Fu Naiyong Gao Fengyuan Che Guangyin Xu Hongxing Han Quanping Su Guozhao Ma |
| author_sort | Qingxi Fu |
| collection | DOAJ |
| description | We examined synergistic effects of inhibiting reactive oxygen species generated from the mitochondria and from nicotinamide adenine dinucleotide phosphate oxidase on neurotoxicity. Primary hippocampal neurons were exposed to amyloid β, and the cells were treated with diazoxide or/and diphenyleneiodonium chloride. We found that the cell viability was decreased significantly after exposure to amyloid β for 72 h with higer reactive oxygen species and malondialdehyde levels, higher caspase-3 and cleaved caspase-3 levels and lower B-cell lymphoma 2 (Bcl-2) level. Both diazoxide and diphenyleneiodonium increased cell viability by inhibiting the increase in reactive oxygen species and caspase-3 activity as well as the decrease in Bcl-2 induced by amyloid β. The combination of diazoxide and diphenyleneiodonium exhibited better protective effects compared to a single treatment. In conclusion, the activation of a mitochondrial potassium channel in combination with the inhibitor of nicotinamide adenine dinucleotide phosphate oxidase exhibit synergistic protective effects against amyloid β neurotoxicity. |
| first_indexed | 2024-12-12T10:47:36Z |
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| id | doaj.art-d09bcfaa405c46699e939c1f6fb2b782 |
| institution | Directory Open Access Journal |
| issn | 0219-6352 1757-448X |
| language | English |
| last_indexed | 2024-12-12T10:47:36Z |
| publishDate | 2019-12-01 |
| publisher | IMR Press |
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| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-d09bcfaa405c46699e939c1f6fb2b7822022-12-22T00:26:51ZengIMR PressJournal of Integrative Neuroscience0219-63521757-448X2019-12-0118444544910.31083/j.jin.2019.04.11741577791324315-850926386Diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicityQingxi Fu0Naiyong Gao1Fengyuan Che2Guangyin Xu3Hongxing Han4Quanping Su5Guozhao Ma6 Deparment of Neurology, Linyi People's Hospital, Shandong Province, 276003, P. R. China Deparment of Neurology, Linyi People's Hospital, Shandong Province, 276003, P. R. China Deparment of Neurology, Linyi People's Hospital, Shandong Province, 276003, P. R. China Deparment of Neurology, Linyi People's Hospital, Shandong Province, 276003, P. R. China Deparment of Neurology, Linyi People's Hospital, Shandong Province, 276003, P. R. China Central Laboratory, Linyi People's Hospital, Shandong Province, 276003, P. R. China Deparment of Neurology, East Hospital, Tongji University School of Medicine, Shanghai, 200120, P. R. China We examined synergistic effects of inhibiting reactive oxygen species generated from the mitochondria and from nicotinamide adenine dinucleotide phosphate oxidase on neurotoxicity. Primary hippocampal neurons were exposed to amyloid β, and the cells were treated with diazoxide or/and diphenyleneiodonium chloride. We found that the cell viability was decreased significantly after exposure to amyloid β for 72 h with higer reactive oxygen species and malondialdehyde levels, higher caspase-3 and cleaved caspase-3 levels and lower B-cell lymphoma 2 (Bcl-2) level. Both diazoxide and diphenyleneiodonium increased cell viability by inhibiting the increase in reactive oxygen species and caspase-3 activity as well as the decrease in Bcl-2 induced by amyloid β. The combination of diazoxide and diphenyleneiodonium exhibited better protective effects compared to a single treatment. In conclusion, the activation of a mitochondrial potassium channel in combination with the inhibitor of nicotinamide adenine dinucleotide phosphate oxidase exhibit synergistic protective effects against amyloid β neurotoxicity. https://jin.imrpress.com/fileup/1757-448X/PDF/1577791324315-850926386.pdf alzheimer's diseasediphenyleneiodoniumpotassium channelreactive oxygen speciesneurotoxicity |
| spellingShingle | Qingxi Fu Naiyong Gao Fengyuan Che Guangyin Xu Hongxing Han Quanping Su Guozhao Ma Diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicity Journal of Integrative Neuroscience alzheimer's disease diphenyleneiodonium potassium channel reactive oxygen species neurotoxicity |
| title | Diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicity |
| title_full | Diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicity |
| title_fullStr | Diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicity |
| title_full_unstemmed | Diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicity |
| title_short | Diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicity |
| title_sort | diphenyleneiodonium chloride synergizes with diazoxide to enhance protection against amyloid β induced neurotoxicity |
| topic | alzheimer's disease diphenyleneiodonium potassium channel reactive oxygen species neurotoxicity |
| url |
https://jin.imrpress.com/fileup/1757-448X/PDF/1577791324315-850926386.pdf
|
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