Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway
Arctiin, a lignan glycoside, is isolated from <i>Arctium lappa</i> L. The anticancer effects of arctiin have been demonstrated in several studies. However, no research has been conducted on the anti-migration effect of arctiin in cervical cancer cells. The present study examined the effe...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/2/365 |
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| author | Chung-Yuan Lee Min-Chieh Hsin Pei-Ni Chen Chiao-Wen Lin Po-Hui Wang Shun-Fa Yang Yi-Hsuan Hsiao |
| author_facet | Chung-Yuan Lee Min-Chieh Hsin Pei-Ni Chen Chiao-Wen Lin Po-Hui Wang Shun-Fa Yang Yi-Hsuan Hsiao |
| author_sort | Chung-Yuan Lee |
| collection | DOAJ |
| description | Arctiin, a lignan glycoside, is isolated from <i>Arctium lappa</i> L. The anticancer effects of arctiin have been demonstrated in several studies. However, no research has been conducted on the anti-migration effect of arctiin in cervical cancer cells. The present study examined the effects of arctiin on cervical cancer cells and investigated the possible molecular mechanism. We demonstrated that arctiin exhibited low cytotoxicity and significantly inhibited cell migration and invasion in human cervical cancer cells. The S100A4 protein expression and mRNA levels were significantly reduced in HeLa and SiHa cells with arctiin treatment. Furthermore, silencing S100A4 by using small interfering RNA reduced cell migration, while overexpression of S100A4 mitigated the migration inhibition imposed by arctiin in cervical cancer cells. Western blotting revealed that arctiin significantly reduced phosphoinositide 3-kinase (PI3K) and phosphorylation of Akt in cervical cancer cells. Moreover, selective Akt induction by an Akt activator, SC-79, reverted cervical cancer cell migration and S100A4 protein expression, which were reduced in response to arctiin. Taken together, these results suggest that arctiin inhibits cervical cancer cell migration and invasion through suppression of S100A4 and the PI3K/Akt pathway. |
| first_indexed | 2024-03-09T21:14:21Z |
| format | Article |
| id | doaj.art-d09d678e9aa14a8ea27ab33fb3c605c8 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T21:14:21Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-d09d678e9aa14a8ea27ab33fb3c605c82023-11-23T21:38:07ZengMDPI AGPharmaceutics1999-49232022-02-0114236510.3390/pharmaceutics14020365Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt PathwayChung-Yuan Lee0Min-Chieh Hsin1Pei-Ni Chen2Chiao-Wen Lin3Po-Hui Wang4Shun-Fa Yang5Yi-Hsuan Hsiao6Department of Obstetrics and Gynecology, Chiayi Chang Gung Memorial Hospital, Chiayi 613, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanInstitute of Medicine, Chung Shan Medical University, Taichung 402, TaiwanSchool of Medicine, Chung Shan Medical University, Taichung 402, TaiwanArctiin, a lignan glycoside, is isolated from <i>Arctium lappa</i> L. The anticancer effects of arctiin have been demonstrated in several studies. However, no research has been conducted on the anti-migration effect of arctiin in cervical cancer cells. The present study examined the effects of arctiin on cervical cancer cells and investigated the possible molecular mechanism. We demonstrated that arctiin exhibited low cytotoxicity and significantly inhibited cell migration and invasion in human cervical cancer cells. The S100A4 protein expression and mRNA levels were significantly reduced in HeLa and SiHa cells with arctiin treatment. Furthermore, silencing S100A4 by using small interfering RNA reduced cell migration, while overexpression of S100A4 mitigated the migration inhibition imposed by arctiin in cervical cancer cells. Western blotting revealed that arctiin significantly reduced phosphoinositide 3-kinase (PI3K) and phosphorylation of Akt in cervical cancer cells. Moreover, selective Akt induction by an Akt activator, SC-79, reverted cervical cancer cell migration and S100A4 protein expression, which were reduced in response to arctiin. Taken together, these results suggest that arctiin inhibits cervical cancer cell migration and invasion through suppression of S100A4 and the PI3K/Akt pathway.https://www.mdpi.com/1999-4923/14/2/365arctiinS100A4migrationcervical cancer |
| spellingShingle | Chung-Yuan Lee Min-Chieh Hsin Pei-Ni Chen Chiao-Wen Lin Po-Hui Wang Shun-Fa Yang Yi-Hsuan Hsiao Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway Pharmaceutics arctiin S100A4 migration cervical cancer |
| title | Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway |
| title_full | Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway |
| title_fullStr | Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway |
| title_full_unstemmed | Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway |
| title_short | Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway |
| title_sort | arctiin inhibits cervical cancer cell migration and invasion through suppression of s100a4 expression via pi3k akt pathway |
| topic | arctiin S100A4 migration cervical cancer |
| url | https://www.mdpi.com/1999-4923/14/2/365 |
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