Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells
<p>Abstract</p> <p>Background</p> <p>Many studies have found that smoking reduces lung function, but the relationship between cigarette smoke and allergic asthma has not been clearly elucidated, particularly the role of mast cells. This study aimed to investigate the ef...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-04-01
|
| Series: | Respiratory Research |
| Online Access: | http://respiratory-research.com/content/12/1/49 |
| _version_ | 1818835078136987648 |
|---|---|
| author | Lee Seung-Hyo Lee Yun Hong Gwan Kwon Eun Kim Dae Ro Jai |
| author_facet | Lee Seung-Hyo Lee Yun Hong Gwan Kwon Eun Kim Dae Ro Jai |
| author_sort | Lee Seung-Hyo |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Many studies have found that smoking reduces lung function, but the relationship between cigarette smoke and allergic asthma has not been clearly elucidated, particularly the role of mast cells. This study aimed to investigate the effects of smoke exposure on allergic asthma and its association with mast cells.</p> <p>Methods</p> <p>BALB/c mice were sensitized and challenged by OVA to induce asthma, and bone marrow-derived mast cells (BMMCs) were stimulated with antigen/antibody reaction. Mice or BMMCs were exposed to cigarette smoke or CSE solution for 1 mo or 6 h, respectively. The recruitment of inflammatory cells into BAL fluid or lung tissues was determined by Diff-Quik or H&E staining, collagen deposition by Sircol assay, penh values by a whole-body plethysmography, co-localization of tryptase and Smad3 by immunohistochemistry, IgE and TGF-β level by ELISA, expressions of Smads proteins, activities of signaling molecules, or TGF-β mRNA by immunoblotting and RT-PCR.</p> <p>Results</p> <p>Cigarette smoke enhanced OVA-specific IgE levels, penh values, recruitment of inflammatory cells including mast cells, expressions of smad family, TGF-β mRNA and proteins, and cytokines, phosphorylations of Smad2 and 3, and MAP kinases, co-localization of tryptase and Smad3, and collagen deposition more than those of BAL cells and lung tissues of OVA-induced allergic mice. CSE solution pretreatment enhanced expressions of TGF-β, Smad3, activities of MAP kinases, NF-κB/AP-1 or PAI-1 more than those of activated-BMMCs.</p> <p>Conclusions</p> <p>The data suggest that smoke exposure enhances antigen-induced mast cell activation via TGF-β/Smad signaling pathways in mouse allergic asthma, and that it exacerbates airway inflammation and remodeling.</p> |
| first_indexed | 2024-12-19T02:44:59Z |
| format | Article |
| id | doaj.art-d0a1734d895b469abf755a408f68d5a3 |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-12-19T02:44:59Z |
| publishDate | 2011-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-d0a1734d895b469abf755a408f68d5a32022-12-21T20:38:58ZengBMCRespiratory Research1465-99212011-04-011214910.1186/1465-9921-12-49Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cellsLee Seung-HyoLee YunHong GwanKwon EunKim DaeRo Jai<p>Abstract</p> <p>Background</p> <p>Many studies have found that smoking reduces lung function, but the relationship between cigarette smoke and allergic asthma has not been clearly elucidated, particularly the role of mast cells. This study aimed to investigate the effects of smoke exposure on allergic asthma and its association with mast cells.</p> <p>Methods</p> <p>BALB/c mice were sensitized and challenged by OVA to induce asthma, and bone marrow-derived mast cells (BMMCs) were stimulated with antigen/antibody reaction. Mice or BMMCs were exposed to cigarette smoke or CSE solution for 1 mo or 6 h, respectively. The recruitment of inflammatory cells into BAL fluid or lung tissues was determined by Diff-Quik or H&E staining, collagen deposition by Sircol assay, penh values by a whole-body plethysmography, co-localization of tryptase and Smad3 by immunohistochemistry, IgE and TGF-β level by ELISA, expressions of Smads proteins, activities of signaling molecules, or TGF-β mRNA by immunoblotting and RT-PCR.</p> <p>Results</p> <p>Cigarette smoke enhanced OVA-specific IgE levels, penh values, recruitment of inflammatory cells including mast cells, expressions of smad family, TGF-β mRNA and proteins, and cytokines, phosphorylations of Smad2 and 3, and MAP kinases, co-localization of tryptase and Smad3, and collagen deposition more than those of BAL cells and lung tissues of OVA-induced allergic mice. CSE solution pretreatment enhanced expressions of TGF-β, Smad3, activities of MAP kinases, NF-κB/AP-1 or PAI-1 more than those of activated-BMMCs.</p> <p>Conclusions</p> <p>The data suggest that smoke exposure enhances antigen-induced mast cell activation via TGF-β/Smad signaling pathways in mouse allergic asthma, and that it exacerbates airway inflammation and remodeling.</p>http://respiratory-research.com/content/12/1/49 |
| spellingShingle | Lee Seung-Hyo Lee Yun Hong Gwan Kwon Eun Kim Dae Ro Jai Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells Respiratory Research |
| title | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
| title_full | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
| title_fullStr | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
| title_full_unstemmed | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
| title_short | Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells |
| title_sort | cigarette smoke exacerbates mouse allergic asthma through smad proteins expressed in mast cells |
| url | http://respiratory-research.com/content/12/1/49 |
| work_keys_str_mv | AT leeseunghyo cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT leeyun cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT honggwan cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT kwoneun cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT kimdae cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells AT rojai cigarettesmokeexacerbatesmouseallergicasthmathroughsmadproteinsexpressedinmastcells |