Stomata characters of sugarcane (<i>Saccharum officinarum</i> L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia
The induction of colchicine mutations is one method of breeding. PT Gunung Madu Plantations, for example, has induced mutations of commercial sugarcane (Saccharum officinarum L.) varieties, however, investigations on the impact of colchicine on stomatal characters have received less attention. There...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2023-09-01
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| Series: | Journal of Tropical Biodiversity and Biotechnology |
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| Online Access: | https://journal.ugm.ac.id/jtbb/article/view/79860 |
| _version_ | 1827312478606852096 |
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| author | Mahfut Mahfut Putri Kendari Admi Syarif Sri Wahyuningsih Endah Susiyanti |
| author_facet | Mahfut Mahfut Putri Kendari Admi Syarif Sri Wahyuningsih Endah Susiyanti |
| author_sort | Mahfut Mahfut |
| collection | DOAJ |
| description | The induction of colchicine mutations is one method of breeding. PT Gunung Madu Plantations, for example, has induced mutations of commercial sugarcane (Saccharum officinarum L.) varieties, however, investigations on the impact of colchicine on stomatal characters have received less attention. Therefore, this study aimed to analyse the stomata character of 21 sugarcane mutants of the GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia with a focused look at stomata aperture width, stomata length and width, number of stomata, stomatal density, and stomata index. The collected data were analysed using cluster and Principal Component Analysis (PCA) through MVSP software. This study showed that all GMP3 mutants had Graminae-type stomata. In terms of stomata length and width, the average size of the GMP3 variety mutant was greater than that of the control. The diversity of stomata characters is fairly high due to differences in stomata size between GMP3 and control mutants. With a similarity index of 0.20, the phenetic analysis of 21 mutants of the GMP3 variety revealed that the relationship between mutants and controls was getting further. A six-character principal component analysis revealed that axis I's total variation accounted for 40.54 percent of the variation and had an eigenvalue of 2.43, whereas axis II's contribution to the variation was 19.02 percent and had an eigenvalue of 1.14. The findings indicate that stomata are excellent taxonomic evidence for identifying and analysing sugarcane varieties induced by colchicine-induced breeding. |
| first_indexed | 2024-04-24T21:44:07Z |
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| institution | Directory Open Access Journal |
| issn | 2540-9573 2540-9581 |
| language | English |
| last_indexed | 2024-04-24T21:44:07Z |
| publishDate | 2023-09-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Journal of Tropical Biodiversity and Biotechnology |
| spelling | doaj.art-d0a81d6cb9864b5fb820a1a1eb982d912024-03-21T03:30:18ZengUniversitas Gadjah MadaJournal of Tropical Biodiversity and Biotechnology2540-95732540-95812023-09-018310.22146/jtbb.7986034392Stomata characters of sugarcane (<i>Saccharum officinarum</i> L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, IndonesiaMahfut Mahfut0Putri Kendari1Admi Syarif2Sri Wahyuningsih3Endah Susiyanti4Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. Jl. Prof., Dr. Ir. Soemantri Brodjonegoro, No. 1. Gedong Meneng, Kec. Rajabasa, Bandar Lampung, Lampung, 35145Postgraduate Student of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. Jl. Prof., Dr. Ir. Soemantri Brodjonegoro, No. 1. Gedong Meneng, Kec. Rajabasa, Bandar Lampung, Lampung, 35145Department of Computer Science, Faculty of Mathematics and Natural Sciences, University of Lampung. Jl. Prof., Dr. Ir. Soemantri Brodjonegoro, No. 1. Gedong Meneng, Kec. Rajabasa, Bandar Lampung, Lampung, 35145Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. Jl. Prof., Dr. Ir. Soemantri Brodjonegoro, No. 1. Gedong Meneng, Kec. Rajabasa, Bandar Lampung, Lampung, 35145Division of Agronomy, Research and Development, PT. Gunung Madu Plantations. KM 90 Terbanggi Besar, Gunung Batin Udik, Terusan Nunyai, Lampung, 34167The induction of colchicine mutations is one method of breeding. PT Gunung Madu Plantations, for example, has induced mutations of commercial sugarcane (Saccharum officinarum L.) varieties, however, investigations on the impact of colchicine on stomatal characters have received less attention. Therefore, this study aimed to analyse the stomata character of 21 sugarcane mutants of the GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia with a focused look at stomata aperture width, stomata length and width, number of stomata, stomatal density, and stomata index. The collected data were analysed using cluster and Principal Component Analysis (PCA) through MVSP software. This study showed that all GMP3 mutants had Graminae-type stomata. In terms of stomata length and width, the average size of the GMP3 variety mutant was greater than that of the control. The diversity of stomata characters is fairly high due to differences in stomata size between GMP3 and control mutants. With a similarity index of 0.20, the phenetic analysis of 21 mutants of the GMP3 variety revealed that the relationship between mutants and controls was getting further. A six-character principal component analysis revealed that axis I's total variation accounted for 40.54 percent of the variation and had an eigenvalue of 2.43, whereas axis II's contribution to the variation was 19.02 percent and had an eigenvalue of 1.14. The findings indicate that stomata are excellent taxonomic evidence for identifying and analysing sugarcane varieties induced by colchicine-induced breeding.https://journal.ugm.ac.id/jtbb/article/view/79860colchicinegmp3indonesiasaccharum officinarum l.stomatasugarcane |
| spellingShingle | Mahfut Mahfut Putri Kendari Admi Syarif Sri Wahyuningsih Endah Susiyanti Stomata characters of sugarcane (<i>Saccharum officinarum</i> L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia Journal of Tropical Biodiversity and Biotechnology colchicine gmp3 indonesia saccharum officinarum l. stomata sugarcane |
| title | Stomata characters of sugarcane (<i>Saccharum officinarum</i> L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia |
| title_full | Stomata characters of sugarcane (<i>Saccharum officinarum</i> L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia |
| title_fullStr | Stomata characters of sugarcane (<i>Saccharum officinarum</i> L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia |
| title_full_unstemmed | Stomata characters of sugarcane (<i>Saccharum officinarum</i> L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia |
| title_short | Stomata characters of sugarcane (<i>Saccharum officinarum</i> L.) mutants of GMP3 variety at PT Gunung Madu Plantations, Lampung, Indonesia |
| title_sort | stomata characters of sugarcane i saccharum officinarum i l mutants of gmp3 variety at pt gunung madu plantations lampung indonesia |
| topic | colchicine gmp3 indonesia saccharum officinarum l. stomata sugarcane |
| url | https://journal.ugm.ac.id/jtbb/article/view/79860 |
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