CBL-Interacting Protein Kinase 2 Improves Salt Tolerance in Soybean (<i>Glycine max</i> L.)
Salt stress severely limits soybean production worldwide. Calcineurin B-like protein-interacting protein kinases (CIPKs) play a pivotal role in a plant’s adaption to salt stress. However, their biological roles in soybean adaption to salt stress remain poorly understood. Here, the <i>GmCIPK2&l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/7/1595 |
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| author | Hui Li Zhen-Ning Liu Qiang Li Wen-Li Zhu Xiao-Hua Wang Ping Xu Xue Cao Xiao-Yu Cui |
| author_facet | Hui Li Zhen-Ning Liu Qiang Li Wen-Li Zhu Xiao-Hua Wang Ping Xu Xue Cao Xiao-Yu Cui |
| author_sort | Hui Li |
| collection | DOAJ |
| description | Salt stress severely limits soybean production worldwide. Calcineurin B-like protein-interacting protein kinases (CIPKs) play a pivotal role in a plant’s adaption to salt stress. However, their biological roles in soybean adaption to salt stress remain poorly understood. Here, the <i>GmCIPK2</i> expression was increased by NaCl and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). <i>GmCIPK2</i>-overexpression <i>Arabidopsis</i> and soybean hairy roots displayed improved salt tolerance, whereas the RNA interference of hairy roots exhibited enhanced salt sensitivity. Further analyses demonstrated that, upon salt stress, <i>GmCIPK2</i> enhanced the proline content and antioxidant enzyme activity and decreased the H<sub>2</sub>O<sub>2</sub> content, malondialdehyde (MDA) content, and Na<sup>+</sup>/K<sup>+</sup> ratios in soybean. Moreover, <i>GmCIPK2</i> promoted the expression of salt- and antioxidant-related genes in response to salt stress. Moreover, the GmCIPK2-interacting sensor, GmCBL4, increased the salt tolerance of soybean hairy roots. Overall, these results suggest that <i>GmCIPK2</i> functions positively in soybean adaption to salt stress. |
| first_indexed | 2024-03-09T03:48:52Z |
| format | Article |
| id | doaj.art-d0aaaaadaf0e4c53ad7a099d87251139 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T03:48:52Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-d0aaaaadaf0e4c53ad7a099d872511392023-12-03T14:30:43ZengMDPI AGAgronomy2073-43952022-07-01127159510.3390/agronomy12071595CBL-Interacting Protein Kinase 2 Improves Salt Tolerance in Soybean (<i>Glycine max</i> L.)Hui Li0Zhen-Ning Liu1Qiang Li2Wen-Li Zhu3Xiao-Hua Wang4Ping Xu5Xue Cao6Xiao-Yu Cui7College of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, ChinaCollege of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, ChinaCollege of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, ChinaCollege of Modern Agriculture, Linyi Vocational University of Science and Technology, Linyi 276000, ChinaCollege of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, ChinaCollege of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, ChinaCollege of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, ChinaCollege of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, ChinaSalt stress severely limits soybean production worldwide. Calcineurin B-like protein-interacting protein kinases (CIPKs) play a pivotal role in a plant’s adaption to salt stress. However, their biological roles in soybean adaption to salt stress remain poorly understood. Here, the <i>GmCIPK2</i> expression was increased by NaCl and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). <i>GmCIPK2</i>-overexpression <i>Arabidopsis</i> and soybean hairy roots displayed improved salt tolerance, whereas the RNA interference of hairy roots exhibited enhanced salt sensitivity. Further analyses demonstrated that, upon salt stress, <i>GmCIPK2</i> enhanced the proline content and antioxidant enzyme activity and decreased the H<sub>2</sub>O<sub>2</sub> content, malondialdehyde (MDA) content, and Na<sup>+</sup>/K<sup>+</sup> ratios in soybean. Moreover, <i>GmCIPK2</i> promoted the expression of salt- and antioxidant-related genes in response to salt stress. Moreover, the GmCIPK2-interacting sensor, GmCBL4, increased the salt tolerance of soybean hairy roots. Overall, these results suggest that <i>GmCIPK2</i> functions positively in soybean adaption to salt stress.https://www.mdpi.com/2073-4395/12/7/1595soybeanCIPKsalt tolerance |
| spellingShingle | Hui Li Zhen-Ning Liu Qiang Li Wen-Li Zhu Xiao-Hua Wang Ping Xu Xue Cao Xiao-Yu Cui CBL-Interacting Protein Kinase 2 Improves Salt Tolerance in Soybean (<i>Glycine max</i> L.) Agronomy soybean CIPK salt tolerance |
| title | CBL-Interacting Protein Kinase 2 Improves Salt Tolerance in Soybean (<i>Glycine max</i> L.) |
| title_full | CBL-Interacting Protein Kinase 2 Improves Salt Tolerance in Soybean (<i>Glycine max</i> L.) |
| title_fullStr | CBL-Interacting Protein Kinase 2 Improves Salt Tolerance in Soybean (<i>Glycine max</i> L.) |
| title_full_unstemmed | CBL-Interacting Protein Kinase 2 Improves Salt Tolerance in Soybean (<i>Glycine max</i> L.) |
| title_short | CBL-Interacting Protein Kinase 2 Improves Salt Tolerance in Soybean (<i>Glycine max</i> L.) |
| title_sort | cbl interacting protein kinase 2 improves salt tolerance in soybean i glycine max i l |
| topic | soybean CIPK salt tolerance |
| url | https://www.mdpi.com/2073-4395/12/7/1595 |
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