Impaired Expression of the Salvador Homolog-1 Gene Is Associated with the Development and Progression of Colorectal Cancer
Salvador homolog-1 (SAV1) is a component of the Hippo pathway that regulates tissue growth and homeostasis by affecting diverse cell processes, including apoptosis, cell division, and differentiation. The aberrant expression of Hippo pathway components has been observed in various human cancers. Thi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/15/24/5771 |
| _version_ | 1797381759776587776 |
|---|---|
| author | Anna Ewa Kowalczyk Bartlomiej Emil Krazinski Aleksandra Piotrowska Jedrzej Grzegrzolka Janusz Godlewski Piotr Dziegiel Zbigniew Kmiec |
| author_facet | Anna Ewa Kowalczyk Bartlomiej Emil Krazinski Aleksandra Piotrowska Jedrzej Grzegrzolka Janusz Godlewski Piotr Dziegiel Zbigniew Kmiec |
| author_sort | Anna Ewa Kowalczyk |
| collection | DOAJ |
| description | Salvador homolog-1 (SAV1) is a component of the Hippo pathway that regulates tissue growth and homeostasis by affecting diverse cell processes, including apoptosis, cell division, and differentiation. The aberrant expression of Hippo pathway components has been observed in various human cancers. This study aimed to examine the expression level of the <i>SAV1</i> gene in colorectal cancer (CRC) and its prognostic value and associations with tumor progression. We obtained matched pairs of tumor tissue and non-cancerous mucosa of the large intestine from 94 CRC patients as well as 40 colon biopsies of healthy subjects collected during screening colonoscopy. The tissue samples and CRC cell lines were quantified for <i>SAV1</i> mRNA levels using the quantitative polymerase chain reaction method, while SAV1 protein expression was estimated in the paired tissues of CRC patients using immunohistochemistry. The average level of <i>SAV1</i> mRNA was decreased in 93.6% of the tumor tissues compared to the corresponding non-cancerous tissues and biopsies of healthy colon mucosa. A downregulated expression of <i>SAV1</i> mRNA was also noted in the CRC cell lines. Although the average SAV1 immunoreactivity was increased in the CRC samples compared to the non-cancerous tissues, a decreased immunoreactivity of the SAV1 protein in the tumor specimens was associated with lymph node involvement and higher TNM disease stage and histological grade. The results of our study suggest that the impaired expression of SAV1 is involved in CRC progression. |
| first_indexed | 2024-03-08T20:56:07Z |
| format | Article |
| id | doaj.art-d0ab819c15d94173b6dfd100ae3dba99 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-08T20:56:07Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-d0ab819c15d94173b6dfd100ae3dba992023-12-22T13:58:49ZengMDPI AGCancers2072-66942023-12-011524577110.3390/cancers15245771Impaired Expression of the Salvador Homolog-1 Gene Is Associated with the Development and Progression of Colorectal CancerAnna Ewa Kowalczyk0Bartlomiej Emil Krazinski1Aleksandra Piotrowska2Jedrzej Grzegrzolka3Janusz Godlewski4Piotr Dziegiel5Zbigniew Kmiec6Department of Human Histology and Embryology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, PolandDepartment of Human Histology and Embryology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDepartment of Human Histology and Embryology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDepartment of Histology, Medical University of Gdansk, 80-210 Gdansk, PolandSalvador homolog-1 (SAV1) is a component of the Hippo pathway that regulates tissue growth and homeostasis by affecting diverse cell processes, including apoptosis, cell division, and differentiation. The aberrant expression of Hippo pathway components has been observed in various human cancers. This study aimed to examine the expression level of the <i>SAV1</i> gene in colorectal cancer (CRC) and its prognostic value and associations with tumor progression. We obtained matched pairs of tumor tissue and non-cancerous mucosa of the large intestine from 94 CRC patients as well as 40 colon biopsies of healthy subjects collected during screening colonoscopy. The tissue samples and CRC cell lines were quantified for <i>SAV1</i> mRNA levels using the quantitative polymerase chain reaction method, while SAV1 protein expression was estimated in the paired tissues of CRC patients using immunohistochemistry. The average level of <i>SAV1</i> mRNA was decreased in 93.6% of the tumor tissues compared to the corresponding non-cancerous tissues and biopsies of healthy colon mucosa. A downregulated expression of <i>SAV1</i> mRNA was also noted in the CRC cell lines. Although the average SAV1 immunoreactivity was increased in the CRC samples compared to the non-cancerous tissues, a decreased immunoreactivity of the SAV1 protein in the tumor specimens was associated with lymph node involvement and higher TNM disease stage and histological grade. The results of our study suggest that the impaired expression of SAV1 is involved in CRC progression.https://www.mdpi.com/2072-6694/15/24/5771SAV1colorectal cancerclinicopathological parametersHippo pathway |
| spellingShingle | Anna Ewa Kowalczyk Bartlomiej Emil Krazinski Aleksandra Piotrowska Jedrzej Grzegrzolka Janusz Godlewski Piotr Dziegiel Zbigniew Kmiec Impaired Expression of the Salvador Homolog-1 Gene Is Associated with the Development and Progression of Colorectal Cancer Cancers SAV1 colorectal cancer clinicopathological parameters Hippo pathway |
| title | Impaired Expression of the Salvador Homolog-1 Gene Is Associated with the Development and Progression of Colorectal Cancer |
| title_full | Impaired Expression of the Salvador Homolog-1 Gene Is Associated with the Development and Progression of Colorectal Cancer |
| title_fullStr | Impaired Expression of the Salvador Homolog-1 Gene Is Associated with the Development and Progression of Colorectal Cancer |
| title_full_unstemmed | Impaired Expression of the Salvador Homolog-1 Gene Is Associated with the Development and Progression of Colorectal Cancer |
| title_short | Impaired Expression of the Salvador Homolog-1 Gene Is Associated with the Development and Progression of Colorectal Cancer |
| title_sort | impaired expression of the salvador homolog 1 gene is associated with the development and progression of colorectal cancer |
| topic | SAV1 colorectal cancer clinicopathological parameters Hippo pathway |
| url | https://www.mdpi.com/2072-6694/15/24/5771 |
| work_keys_str_mv | AT annaewakowalczyk impairedexpressionofthesalvadorhomolog1geneisassociatedwiththedevelopmentandprogressionofcolorectalcancer AT bartlomiejemilkrazinski impairedexpressionofthesalvadorhomolog1geneisassociatedwiththedevelopmentandprogressionofcolorectalcancer AT aleksandrapiotrowska impairedexpressionofthesalvadorhomolog1geneisassociatedwiththedevelopmentandprogressionofcolorectalcancer AT jedrzejgrzegrzolka impairedexpressionofthesalvadorhomolog1geneisassociatedwiththedevelopmentandprogressionofcolorectalcancer AT januszgodlewski impairedexpressionofthesalvadorhomolog1geneisassociatedwiththedevelopmentandprogressionofcolorectalcancer AT piotrdziegiel impairedexpressionofthesalvadorhomolog1geneisassociatedwiththedevelopmentandprogressionofcolorectalcancer AT zbigniewkmiec impairedexpressionofthesalvadorhomolog1geneisassociatedwiththedevelopmentandprogressionofcolorectalcancer |