A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2
Abstract As the worldwide spreading epidemic of SARS-CoV-2, quick inspection and quarantine of passengers for SARS-CoV-2 infection are essential for controlling the spread of SARS-CoV-2, especially the cross-border transmission. This study reports a SARS-CoV-2 genome sequencing method based on a re-...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-03-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-023-02000-7 |
| _version_ | 1797865635468804096 |
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| author | Runzi Qi Gang Wang Xu Wang Cheng Li Lei Huang Weixi Xiao Bing Shao Chunya Zhou Xun Ding Feng Li Wei Zhou |
| author_facet | Runzi Qi Gang Wang Xu Wang Cheng Li Lei Huang Weixi Xiao Bing Shao Chunya Zhou Xun Ding Feng Li Wei Zhou |
| author_sort | Runzi Qi |
| collection | DOAJ |
| description | Abstract As the worldwide spreading epidemic of SARS-CoV-2, quick inspection and quarantine of passengers for SARS-CoV-2 infection are essential for controlling the spread of SARS-CoV-2, especially the cross-border transmission. This study reports a SARS-CoV-2 genome sequencing method based on a re-sequencing tiling array successfully used in border inspection and quarantine. The tiling array chip has four cores, with one core of 240,000 probes dedicated to the whole genome sequencing of the SAR-CoV-2 genome. The assay protocol has been improved to reduce the detection time to within one day and can detect 96 samples in parallel. The detection accuracy has been validated. This fast and simple procedure is also of low cost and high accuracy, and it is particularly suitable for the rapid tracking of viral genetic variants in custom inspection applications. Combining these properties means this method has significant application potential in the clinical investigation and quarantine of SARS-CoV-2. We used this SARS-CoV-2 genome re-sequencing tiling array to inspect and quarantine China's entry and exit ports in the Zhejiang Province. From November 2020 to January 2022, we observed the gradual shift of SARS-CoV-2 variants from the D614G type to the Delta Variant, and then to the dominance of the Omicron variant recently, consistently with the global emergency pattern of the new SARS-CoV-2 variant. |
| first_indexed | 2024-04-09T23:11:16Z |
| format | Article |
| id | doaj.art-d0acda89cf444d378ac83144b2d98ca3 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-09T23:11:16Z |
| publishDate | 2023-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-d0acda89cf444d378ac83144b2d98ca32023-03-22T10:24:53ZengBMCVirology Journal1743-422X2023-03-012011610.1186/s12985-023-02000-7A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2Runzi Qi0Gang Wang1Xu Wang2Cheng Li3Lei Huang4Weixi Xiao5Bing Shao6Chunya Zhou7Xun Ding8Feng Li9Wei Zhou10Hangzhou Xiaoshan Airport Customs of the People’s Republic of ChinaHangzhou International Travel Healthcare CenterCentrillion Technology (Hangzhou) Co., LtdHangzhou Customs Logistics Management CenterZhoushan International Travel Healthcare Center ClinicCentrillion Technology (Hangzhou) Co., LtdCentrillion Technology (Hangzhou) Co., LtdHangzhou Xiaoshan Airport Customs of the People’s Republic of ChinaCentrillion TechnologiesCentrillion Technology (Hangzhou) Co., LtdCentrillion TechnologiesAbstract As the worldwide spreading epidemic of SARS-CoV-2, quick inspection and quarantine of passengers for SARS-CoV-2 infection are essential for controlling the spread of SARS-CoV-2, especially the cross-border transmission. This study reports a SARS-CoV-2 genome sequencing method based on a re-sequencing tiling array successfully used in border inspection and quarantine. The tiling array chip has four cores, with one core of 240,000 probes dedicated to the whole genome sequencing of the SAR-CoV-2 genome. The assay protocol has been improved to reduce the detection time to within one day and can detect 96 samples in parallel. The detection accuracy has been validated. This fast and simple procedure is also of low cost and high accuracy, and it is particularly suitable for the rapid tracking of viral genetic variants in custom inspection applications. Combining these properties means this method has significant application potential in the clinical investigation and quarantine of SARS-CoV-2. We used this SARS-CoV-2 genome re-sequencing tiling array to inspect and quarantine China's entry and exit ports in the Zhejiang Province. From November 2020 to January 2022, we observed the gradual shift of SARS-CoV-2 variants from the D614G type to the Delta Variant, and then to the dominance of the Omicron variant recently, consistently with the global emergency pattern of the new SARS-CoV-2 variant.https://doi.org/10.1186/s12985-023-02000-7Full genome tiling arraySARS-CoV-2PortsInspection and quarantineVariation |
| spellingShingle | Runzi Qi Gang Wang Xu Wang Cheng Li Lei Huang Weixi Xiao Bing Shao Chunya Zhou Xun Ding Feng Li Wei Zhou A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2 Virology Journal Full genome tiling array SARS-CoV-2 Ports Inspection and quarantine Variation |
| title | A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2 |
| title_full | A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2 |
| title_fullStr | A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2 |
| title_full_unstemmed | A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2 |
| title_short | A full genome tiling array enhanced the inspection and quarantine of SARS-CoV-2 |
| title_sort | full genome tiling array enhanced the inspection and quarantine of sars cov 2 |
| topic | Full genome tiling array SARS-CoV-2 Ports Inspection and quarantine Variation |
| url | https://doi.org/10.1186/s12985-023-02000-7 |
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