Unusual patterns of hybridization involving two alpine Salvia species: Absence of both F1 and backcrossed hybrids
Natural hybridization plays an important role in speciation; however, we still know little about the mechanisms underlying the early stages of hybrid speciation. Hybrid zones are commonly dominated by F1s, or backcrosses, which impedes further speciation. In the present study, morphological traits a...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1010577/full |
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| author | Yuhang Chang Yuhang Chang Shengxuan Zhao Hanwen Xiao Detuan Liu Detuan Liu Yanbo Huang Yukun Wei Yukun Wei Yongpeng Ma |
| author_facet | Yuhang Chang Yuhang Chang Shengxuan Zhao Hanwen Xiao Detuan Liu Detuan Liu Yanbo Huang Yukun Wei Yukun Wei Yongpeng Ma |
| author_sort | Yuhang Chang |
| collection | DOAJ |
| description | Natural hybridization plays an important role in speciation; however, we still know little about the mechanisms underlying the early stages of hybrid speciation. Hybrid zones are commonly dominated by F1s, or backcrosses, which impedes further speciation. In the present study, morphological traits and double digest restriction‐site associated DNA sequencing (ddRAD-seq) data have been used to confirm natural hybridization between Salvia flava and S. castanea, the first case of identification of natural hybridization using combined phenotypic and molecular evidence in the East Asian clade of Salvia. We further examined several reproductive barriers in both pre-zygotic and post-zygotic reproductive stages to clarify the causes and consequences of the hybridization pattern. Our results revealed that reproductive isolation between the two species was strong despite the occurrence of hybridization. Interestingly, we found that most of the hybrids were likely to be F2s. This is a very unusual pattern of hybridization, and has rarely been reported before. The prevalence of geitonogamy within these self-compatible hybrids due to short distance foraging by pollinators might explain the origin of this unusual pattern. F2s can self-breed and develop further, therefore, we might be witnessing the early stages of hybrid speciation. Our study provides a new case for understanding the diversification of plants on the Qinghai-Tibet Plateau. |
| first_indexed | 2024-04-11T19:35:32Z |
| format | Article |
| id | doaj.art-d0ad3cbc8d73471e859928cb688ea451 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T19:35:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d0ad3cbc8d73471e859928cb688ea4512022-12-22T04:06:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10105771010577Unusual patterns of hybridization involving two alpine Salvia species: Absence of both F1 and backcrossed hybridsYuhang Chang0Yuhang Chang1Shengxuan Zhao2Hanwen Xiao3Detuan Liu4Detuan Liu5Yanbo Huang6Yukun Wei7Yukun Wei8Yongpeng Ma9Yunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCollege of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaEastern China Conservation Center for Wild Endangered Plant Resources, Shanghai Chenshan Botanical Garden, Shanghai, ChinaYunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaEastern China Conservation Center for Wild Endangered Plant Resources, Shanghai Chenshan Botanical Garden, Shanghai, ChinaEastern China Conservation Center for Wild Endangered Plant Resources, Shanghai Chenshan Botanical Garden, Shanghai, ChinaShanghai Botanical Garden, Shanghai, ChinaYunnan Key Laboratory for Integrative Conservation of Plant Species with Extremely Small Populations, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, ChinaNatural hybridization plays an important role in speciation; however, we still know little about the mechanisms underlying the early stages of hybrid speciation. Hybrid zones are commonly dominated by F1s, or backcrosses, which impedes further speciation. In the present study, morphological traits and double digest restriction‐site associated DNA sequencing (ddRAD-seq) data have been used to confirm natural hybridization between Salvia flava and S. castanea, the first case of identification of natural hybridization using combined phenotypic and molecular evidence in the East Asian clade of Salvia. We further examined several reproductive barriers in both pre-zygotic and post-zygotic reproductive stages to clarify the causes and consequences of the hybridization pattern. Our results revealed that reproductive isolation between the two species was strong despite the occurrence of hybridization. Interestingly, we found that most of the hybrids were likely to be F2s. This is a very unusual pattern of hybridization, and has rarely been reported before. The prevalence of geitonogamy within these self-compatible hybrids due to short distance foraging by pollinators might explain the origin of this unusual pattern. F2s can self-breed and develop further, therefore, we might be witnessing the early stages of hybrid speciation. Our study provides a new case for understanding the diversification of plants on the Qinghai-Tibet Plateau.https://www.frontiersin.org/articles/10.3389/fpls.2022.1010577/fullhybridizationSalviaRAD-seqspeciationreproductive isolation barriersethological isolation |
| spellingShingle | Yuhang Chang Yuhang Chang Shengxuan Zhao Hanwen Xiao Detuan Liu Detuan Liu Yanbo Huang Yukun Wei Yukun Wei Yongpeng Ma Unusual patterns of hybridization involving two alpine Salvia species: Absence of both F1 and backcrossed hybrids Frontiers in Plant Science hybridization Salvia RAD-seq speciation reproductive isolation barriers ethological isolation |
| title | Unusual patterns of hybridization involving two alpine Salvia species: Absence of both F1 and backcrossed hybrids |
| title_full | Unusual patterns of hybridization involving two alpine Salvia species: Absence of both F1 and backcrossed hybrids |
| title_fullStr | Unusual patterns of hybridization involving two alpine Salvia species: Absence of both F1 and backcrossed hybrids |
| title_full_unstemmed | Unusual patterns of hybridization involving two alpine Salvia species: Absence of both F1 and backcrossed hybrids |
| title_short | Unusual patterns of hybridization involving two alpine Salvia species: Absence of both F1 and backcrossed hybrids |
| title_sort | unusual patterns of hybridization involving two alpine salvia species absence of both f1 and backcrossed hybrids |
| topic | hybridization Salvia RAD-seq speciation reproductive isolation barriers ethological isolation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1010577/full |
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