Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancer
Objective The objective was to explore the expression and potential functions of long noncoding RNA (lncRNA) and mRNAs in human breast cancer (BC). Methods Differentially expressed lncRNAs and mRNAs were identified and annotated in BC tissues by using the Agilent human lncRNA assay (Agilent Technolo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-09-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/0300060520973137 |
| _version_ | 1818596146504794112 |
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| author | Jingyun Guo Huining Lian Minfeng Liu Jianyu Dong Zhaoze Guo Jinlamao Yang Changsheng Ye |
| author_facet | Jingyun Guo Huining Lian Minfeng Liu Jianyu Dong Zhaoze Guo Jinlamao Yang Changsheng Ye |
| author_sort | Jingyun Guo |
| collection | DOAJ |
| description | Objective The objective was to explore the expression and potential functions of long noncoding RNA (lncRNA) and mRNAs in human breast cancer (BC). Methods Differentially expressed lncRNAs and mRNAs were identified and annotated in BC tissues by using the Agilent human lncRNA assay (Agilent Technologies, Santa Clara, CA, USA) and RNA sequencing. After identification of lncRNAs and mRNAs through quantitative reverse transcription polymerase chain reaction, we conducted a series of functional experiments to confirm the effects of knockdown of one lncRNA, TCONS_00029809, on the progression of BC. Results We discovered 238 lncRNAs and 200 mRNAs that were differentially expressed in BC tissues and para-carcinoma tissue. We showed that differentially expressed mRNAs were related to biological adhesion and biological regulation and mainly enriched in cytokine-cytokine receptor interaction, metabolic pathways, and PI3K-Akt signaling pathway. We created a protein–protein interaction network to analyze the proteins enriched in these pathways. We demonstrated that silencing of TCONS_00029809 remarkably inhibited proliferation, invasion, and migration of BC cells, and accelerated their apoptosis. Conclusions We identified a large number of differentially expressed lncRNAs and mRNAs, which provide data useful in understanding BC carcinogenesis. The lncRNA TCONS_00029809 may be involved in the development of BC. |
| first_indexed | 2024-12-16T11:27:16Z |
| format | Article |
| id | doaj.art-d0ad59733bfa47b39f3a7ca49ec618bd |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-12-16T11:27:16Z |
| publishDate | 2021-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-d0ad59733bfa47b39f3a7ca49ec618bd2022-12-21T22:33:20ZengSAGE PublishingJournal of International Medical Research1473-23002021-09-014910.1177/0300060520973137Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancerJingyun GuoHuining LianMinfeng LiuJianyu DongZhaoze GuoJinlamao YangChangsheng YeObjective The objective was to explore the expression and potential functions of long noncoding RNA (lncRNA) and mRNAs in human breast cancer (BC). Methods Differentially expressed lncRNAs and mRNAs were identified and annotated in BC tissues by using the Agilent human lncRNA assay (Agilent Technologies, Santa Clara, CA, USA) and RNA sequencing. After identification of lncRNAs and mRNAs through quantitative reverse transcription polymerase chain reaction, we conducted a series of functional experiments to confirm the effects of knockdown of one lncRNA, TCONS_00029809, on the progression of BC. Results We discovered 238 lncRNAs and 200 mRNAs that were differentially expressed in BC tissues and para-carcinoma tissue. We showed that differentially expressed mRNAs were related to biological adhesion and biological regulation and mainly enriched in cytokine-cytokine receptor interaction, metabolic pathways, and PI3K-Akt signaling pathway. We created a protein–protein interaction network to analyze the proteins enriched in these pathways. We demonstrated that silencing of TCONS_00029809 remarkably inhibited proliferation, invasion, and migration of BC cells, and accelerated their apoptosis. Conclusions We identified a large number of differentially expressed lncRNAs and mRNAs, which provide data useful in understanding BC carcinogenesis. The lncRNA TCONS_00029809 may be involved in the development of BC.https://doi.org/10.1177/0300060520973137 |
| spellingShingle | Jingyun Guo Huining Lian Minfeng Liu Jianyu Dong Zhaoze Guo Jinlamao Yang Changsheng Ye Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancer Journal of International Medical Research |
| title | Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancer |
| title_full | Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancer |
| title_fullStr | Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancer |
| title_full_unstemmed | Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancer |
| title_short | Integrated analyses of long noncoding RNAs and mRNAs in the progression of breast cancer |
| title_sort | integrated analyses of long noncoding rnas and mrnas in the progression of breast cancer |
| url | https://doi.org/10.1177/0300060520973137 |
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