Mobile colistin resistance gene mcr-1 detected on an IncI1 plasmid in Escherichia coli from meat
Objectives: Mobile colistin resistance (mcr) genes encoded on conjugative plasmids, although described only relatively recently, have been reported globally both in humans and livestock. The genes are often associated with the insertion sequence ISApl1 that can transpose the genes to novel genetic l...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520302241 |
| _version_ | 1819065535870009344 |
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| author | Michael S.M. Brouwer Richard N. Goodman Arie Kant Dik Mevius Enas Newire Adam P. Roberts Kees T. Veldman |
| author_facet | Michael S.M. Brouwer Richard N. Goodman Arie Kant Dik Mevius Enas Newire Adam P. Roberts Kees T. Veldman |
| author_sort | Michael S.M. Brouwer |
| collection | DOAJ |
| description | Objectives: Mobile colistin resistance (mcr) genes encoded on conjugative plasmids, although described only relatively recently, have been reported globally both in humans and livestock. The genes are often associated with the insertion sequence ISApl1 that can transpose the genes to novel genetic locations. Since its first report, multiple variants of mcr have been discovered in a variety of genetic locations in Escherichia coli, in plasmids and integrated into the chromosome. Methods: Using hybrid assembly of short-read and long-read whole-genome sequencing data, the presence ofmcr-1 was confirmed on an IncI1 plasmid in E. coli. In vitro conjugation assays were performed to determine the potential to transfer between strains. Genetic comparison with previously reported IncI1 plasmids was performed. Results: The genomic sequence identified thatmcr-1 is present on a complete IncI1 plasmid. Comparison with previously reported extended-spectrum β-lactamase (ESBL)-encoding plasmids from E. coli in the Netherlands from the same time period indicated a distinct lineage for this plasmid. Conclusions: The observation ofmcr-1 on an IncI1 plasmid confirms that the genetic region of this gene is actively transposed between genetic locations. This active transposition has consequences for the study of the epidemiology of mcr in populations. |
| first_indexed | 2024-12-21T15:48:01Z |
| format | Article |
| id | doaj.art-d0aeae420f1e4b578d1aacce2072d252 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-21T15:48:01Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-d0aeae420f1e4b578d1aacce2072d2522022-12-21T18:58:18ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-12-0123145148Mobile colistin resistance gene mcr-1 detected on an IncI1 plasmid in Escherichia coli from meatMichael S.M. Brouwer0Richard N. Goodman1Arie Kant2Dik Mevius3Enas Newire4Adam P. Roberts5Kees T. Veldman6Wageningen Bioveterinary Research, Lelystad, The Netherlands; Corresponding author at: Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, Wageningen University and Research, P.O. Box Postbus 65, 8200AB Lelystad, The Netherlands.Liverpool School of Tropical Medicine, Liverpool, UKWageningen Bioveterinary Research, Lelystad, The NetherlandsWageningen Bioveterinary Research, Lelystad, The NetherlandsInstitute of Systems, Molecular & Integrative Biology, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, UKLiverpool School of Tropical Medicine, Liverpool, UKWageningen Bioveterinary Research, Lelystad, The NetherlandsObjectives: Mobile colistin resistance (mcr) genes encoded on conjugative plasmids, although described only relatively recently, have been reported globally both in humans and livestock. The genes are often associated with the insertion sequence ISApl1 that can transpose the genes to novel genetic locations. Since its first report, multiple variants of mcr have been discovered in a variety of genetic locations in Escherichia coli, in plasmids and integrated into the chromosome. Methods: Using hybrid assembly of short-read and long-read whole-genome sequencing data, the presence ofmcr-1 was confirmed on an IncI1 plasmid in E. coli. In vitro conjugation assays were performed to determine the potential to transfer between strains. Genetic comparison with previously reported IncI1 plasmids was performed. Results: The genomic sequence identified thatmcr-1 is present on a complete IncI1 plasmid. Comparison with previously reported extended-spectrum β-lactamase (ESBL)-encoding plasmids from E. coli in the Netherlands from the same time period indicated a distinct lineage for this plasmid. Conclusions: The observation ofmcr-1 on an IncI1 plasmid confirms that the genetic region of this gene is actively transposed between genetic locations. This active transposition has consequences for the study of the epidemiology of mcr in populations.http://www.sciencedirect.com/science/article/pii/S2213716520302241Mobile colistin resistance1 mcr-1IncI1 plasmidConjugation |
| spellingShingle | Michael S.M. Brouwer Richard N. Goodman Arie Kant Dik Mevius Enas Newire Adam P. Roberts Kees T. Veldman Mobile colistin resistance gene mcr-1 detected on an IncI1 plasmid in Escherichia coli from meat Journal of Global Antimicrobial Resistance Mobile colistin resistance 1 mcr-1 IncI1 plasmid Conjugation |
| title | Mobile colistin resistance gene mcr-1 detected on an IncI1 plasmid in Escherichia coli from meat |
| title_full | Mobile colistin resistance gene mcr-1 detected on an IncI1 plasmid in Escherichia coli from meat |
| title_fullStr | Mobile colistin resistance gene mcr-1 detected on an IncI1 plasmid in Escherichia coli from meat |
| title_full_unstemmed | Mobile colistin resistance gene mcr-1 detected on an IncI1 plasmid in Escherichia coli from meat |
| title_short | Mobile colistin resistance gene mcr-1 detected on an IncI1 plasmid in Escherichia coli from meat |
| title_sort | mobile colistin resistance gene mcr 1 detected on an inci1 plasmid in escherichia coli from meat |
| topic | Mobile colistin resistance 1 mcr-1 IncI1 plasmid Conjugation |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520302241 |
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