Thymoquinone disrupts the microtubule dynamics in fission yeast <em>Schizosaccharomyces pombe</em>
Mad2 deletion strain of <em>Schizosaccharomyces pombe</em> was found to be sensitive to thymoquinone, a signature molecule present in <em>Nigella sativa</em> in a dose-dependent manner. Mad2 protein is an indispensable part of mitotic spindle checkpoint complex and is require...
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AIMS Press
2016-11-01
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| Series: | AIMS Genetics |
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| Online Access: | http://www.aimspress.com/Genetics/article/1093/fulltext.html |
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| author | Nusrat Masood Saman Khan Suaib Luqman Shakil Ahmed |
| author_facet | Nusrat Masood Saman Khan Suaib Luqman Shakil Ahmed |
| author_sort | Nusrat Masood |
| collection | DOAJ |
| description | Mad2 deletion strain of <em>Schizosaccharomyces pombe</em> was found to be sensitive to thymoquinone, a signature molecule present in <em>Nigella sativa</em> in a dose-dependent manner. Mad2 protein is an indispensable part of mitotic spindle checkpoint complex and is required for the cell cycle arrest in response to the spindle defects. Although the expression of α tubulin was not affected in thymoquinone treated cells, but the expression of β-tubulin was reduced. Further, the absence of microtubule in thymoquinone treated cells suggests its involvement in tubulin polymerization. Molecular docking studies revealed that thymoquinone specifically binds to β-tubulin near the Taxotere binding site of Tub1 (Tubulin α-β dimer). These studies additionally showed that thymoquinone interacts with the residues present in chain B, which is an inherent part of Mad2 protein of mitotic checkpoint complex (MCC). We concluded that the thymoquinone disrupts the microtubule polymerization that leads to the requirement of spindle checkpoint protein for the cell survival. |
| first_indexed | 2024-12-14T15:18:18Z |
| format | Article |
| id | doaj.art-d0b512178aa448e3913222b63ed5ed40 |
| institution | Directory Open Access Journal |
| issn | 2377-1143 |
| language | English |
| last_indexed | 2024-12-14T15:18:18Z |
| publishDate | 2016-11-01 |
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| record_format | Article |
| series | AIMS Genetics |
| spelling | doaj.art-d0b512178aa448e3913222b63ed5ed402022-12-21T22:56:15ZengAIMS PressAIMS Genetics2377-11432016-11-013423925110.3934/genet.2016.4.239genetics-03-00239Thymoquinone disrupts the microtubule dynamics in fission yeast <em>Schizosaccharomyces pombe</em>Nusrat Masood0Saman Khan1Suaib Luqman2Shakil Ahmed3Molecular Bioprospection Department of Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, Uttar Pradesh, India.Molecular and Structural Biology Division, CSIR—Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow-226031, India,Molecular Bioprospection Department of Biotechnology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, Uttar Pradesh, India.Molecular and Structural Biology Division, CSIR—Central Drug Research Institute, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow-226031, India,Mad2 deletion strain of <em>Schizosaccharomyces pombe</em> was found to be sensitive to thymoquinone, a signature molecule present in <em>Nigella sativa</em> in a dose-dependent manner. Mad2 protein is an indispensable part of mitotic spindle checkpoint complex and is required for the cell cycle arrest in response to the spindle defects. Although the expression of α tubulin was not affected in thymoquinone treated cells, but the expression of β-tubulin was reduced. Further, the absence of microtubule in thymoquinone treated cells suggests its involvement in tubulin polymerization. Molecular docking studies revealed that thymoquinone specifically binds to β-tubulin near the Taxotere binding site of Tub1 (Tubulin α-β dimer). These studies additionally showed that thymoquinone interacts with the residues present in chain B, which is an inherent part of Mad2 protein of mitotic checkpoint complex (MCC). We concluded that the thymoquinone disrupts the microtubule polymerization that leads to the requirement of spindle checkpoint protein for the cell survival.http://www.aimspress.com/Genetics/article/1093/fulltext.htmlThymoquinonespindle assembly checkpointMad2Tubulin |
| spellingShingle | Nusrat Masood Saman Khan Suaib Luqman Shakil Ahmed Thymoquinone disrupts the microtubule dynamics in fission yeast <em>Schizosaccharomyces pombe</em> AIMS Genetics Thymoquinone spindle assembly checkpoint Mad2 Tubulin |
| title | Thymoquinone disrupts the microtubule dynamics in fission yeast <em>Schizosaccharomyces pombe</em> |
| title_full | Thymoquinone disrupts the microtubule dynamics in fission yeast <em>Schizosaccharomyces pombe</em> |
| title_fullStr | Thymoquinone disrupts the microtubule dynamics in fission yeast <em>Schizosaccharomyces pombe</em> |
| title_full_unstemmed | Thymoquinone disrupts the microtubule dynamics in fission yeast <em>Schizosaccharomyces pombe</em> |
| title_short | Thymoquinone disrupts the microtubule dynamics in fission yeast <em>Schizosaccharomyces pombe</em> |
| title_sort | thymoquinone disrupts the microtubule dynamics in fission yeast em schizosaccharomyces pombe em |
| topic | Thymoquinone spindle assembly checkpoint Mad2 Tubulin |
| url | http://www.aimspress.com/Genetics/article/1093/fulltext.html |
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