Ovarian responses to undernutrition in pregnant ewes, USA
<p>Abstract</p> <p>In most mammals oogonia proliferate by mitosis and begin meiotic development during fetal life. Previous studies indicated that there is a delay in the progression to the first stage of meiotic arrest in germ cells of female fetuses of undernourished ewes. We rep...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2003-02-01
|
| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://www.RBEj.com/content/1/1/6 |
| _version_ | 1811251000544067584 |
|---|---|
| author | Vonnahme Kimberly A Van Kirk Edward A Murdoch William J Ford Stephen P |
| author_facet | Vonnahme Kimberly A Van Kirk Edward A Murdoch William J Ford Stephen P |
| author_sort | Vonnahme Kimberly A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>In most mammals oogonia proliferate by mitosis and begin meiotic development during fetal life. Previous studies indicated that there is a delay in the progression to the first stage of meiotic arrest in germ cells of female fetuses of undernourished ewes. We report that underfeeding (50% NRC requirement beginning on Day 28 of pregnancy) provokes an increase in oxidative base lesions within DNA of mid-gestational (Day 78) fetal oogonia; this condition was associated with up-regulation of the tumor suppressor/cell-cycle arrest modulator p53, antiapoptotic factor Bcl-2, and base-excision repair polymerase β. Fetal ovarian weights and germ cell concentrations were not altered by nutrient deprivation. Ovaries of ewes on control diets (100% NRC) contained more tertiary follicles than their restricted counterparts; however, peripheral venous estradiol-17β was not different between groups. There was no effect of treatment on p53 accumulation in maternal oocytes. Luteal structure-function was not perturbed by undernutrition. No fetal losses were attributed to the dietary restriction. It is proposed that DNA of interphase fetal oogonia is vulnerable to oxidative insults perpetrated by a nutritional stress to the dam, and that multiple/integrated adaptive molecular response mechanisms of cell-cycle inhibition (providing the time required for base repairs) and survival hence sustain the genomic integrity and population stability of the germline.</p> |
| first_indexed | 2024-04-12T16:13:08Z |
| format | Article |
| id | doaj.art-d0b562ba39194590b9190b2fef24a321 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-12T16:13:08Z |
| publishDate | 2003-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-d0b562ba39194590b9190b2fef24a3212022-12-22T03:25:50ZengBMCReproductive Biology and Endocrinology1477-78272003-02-0111610.1186/1477-7827-1-6Ovarian responses to undernutrition in pregnant ewes, USAVonnahme Kimberly AVan Kirk Edward AMurdoch William JFord Stephen P<p>Abstract</p> <p>In most mammals oogonia proliferate by mitosis and begin meiotic development during fetal life. Previous studies indicated that there is a delay in the progression to the first stage of meiotic arrest in germ cells of female fetuses of undernourished ewes. We report that underfeeding (50% NRC requirement beginning on Day 28 of pregnancy) provokes an increase in oxidative base lesions within DNA of mid-gestational (Day 78) fetal oogonia; this condition was associated with up-regulation of the tumor suppressor/cell-cycle arrest modulator p53, antiapoptotic factor Bcl-2, and base-excision repair polymerase β. Fetal ovarian weights and germ cell concentrations were not altered by nutrient deprivation. Ovaries of ewes on control diets (100% NRC) contained more tertiary follicles than their restricted counterparts; however, peripheral venous estradiol-17β was not different between groups. There was no effect of treatment on p53 accumulation in maternal oocytes. Luteal structure-function was not perturbed by undernutrition. No fetal losses were attributed to the dietary restriction. It is proposed that DNA of interphase fetal oogonia is vulnerable to oxidative insults perpetrated by a nutritional stress to the dam, and that multiple/integrated adaptive molecular response mechanisms of cell-cycle inhibition (providing the time required for base repairs) and survival hence sustain the genomic integrity and population stability of the germline.</p>http://www.RBEj.com/content/1/1/6sheepnutritionpregnancyfetal oogenesisfolliclecorpus luteum |
| spellingShingle | Vonnahme Kimberly A Van Kirk Edward A Murdoch William J Ford Stephen P Ovarian responses to undernutrition in pregnant ewes, USA Reproductive Biology and Endocrinology sheep nutrition pregnancy fetal oogenesis follicle corpus luteum |
| title | Ovarian responses to undernutrition in pregnant ewes, USA |
| title_full | Ovarian responses to undernutrition in pregnant ewes, USA |
| title_fullStr | Ovarian responses to undernutrition in pregnant ewes, USA |
| title_full_unstemmed | Ovarian responses to undernutrition in pregnant ewes, USA |
| title_short | Ovarian responses to undernutrition in pregnant ewes, USA |
| title_sort | ovarian responses to undernutrition in pregnant ewes usa |
| topic | sheep nutrition pregnancy fetal oogenesis follicle corpus luteum |
| url | http://www.RBEj.com/content/1/1/6 |
| work_keys_str_mv | AT vonnahmekimberlya ovarianresponsestoundernutritioninpregnantewesusa AT vankirkedwarda ovarianresponsestoundernutritioninpregnantewesusa AT murdochwilliamj ovarianresponsestoundernutritioninpregnantewesusa AT fordstephenp ovarianresponsestoundernutritioninpregnantewesusa |