Effect of EGb761 on light-damaged retinal pigment epithelial cells
<b>AIM:</b>To investigate the protective mechanism of Gingko Biloba extract (EGb761) on the ability of retinal pigment epithelial (RPE) cells to resist light-induced damage in a comparative proteomics study.<b>METHODS:</b>Human RPE cells (ARPE-19) were randomly distributed to...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-02-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949451/ |
| _version_ | 1811308845938507776 |
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| author | Yun-Yun Zhou Chang-Zheng Chen Yu Su Lu Li Zuo-Hui-Zi Yi Hang Qi Ming Weng Yi-Qiao Xing |
| author_facet | Yun-Yun Zhou Chang-Zheng Chen Yu Su Lu Li Zuo-Hui-Zi Yi Hang Qi Ming Weng Yi-Qiao Xing |
| author_sort | Yun-Yun Zhou |
| collection | DOAJ |
| description | <b>AIM:</b>To investigate the protective mechanism of Gingko Biloba extract (EGb761) on the ability of retinal pigment epithelial (RPE) cells to resist light-induced damage in a comparative proteomics study.<b>METHODS:</b>Human RPE cells (ARPE-19) were randomly distributed to one of three groups:normal control (NC group) and light-damaged model without or with EGb761 group (M and ME groups, respectively). The light-damaged model was formed by exposing to white light (2 200±300)lx for 6h. The RPE cells in ME group were conducted with EGb 761 (100μg/mL) before light exposure. The soluble cellular proteins extracting from each groups were separated by two-dimensional electrophoresis and stained by silver staining. Different proteins in the profiles of the gels were analyzed by Image Master Software. Two-fold expressing protein spots were identified by Matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry.<b>RESULTS:</b> NC, M and ME groups displayed 1 892±71, 2 145±23 and 2 216±85 protein spots, respectively. We identified 33 proteins with different expression levels between the NC and M groups, 25 proteins between the M and ME groups, and 11 proteins between the NC and ME groups. MALDI-TOF/TOF mass spectrometry successfully identified 16 proteins, including metabolic enzymes, cytoskeletal proteins, anti-oxidation proteins, and others.<b>CONCLUSION:</b>Differences in some important proteins, such as cathepsin B, heat shock protein, and cytochrome creductase, indicated that multiple pathways may be induced in light-damaged RPE cells and the protective effect of EGb761. |
| first_indexed | 2024-04-13T09:30:35Z |
| format | Article |
| id | doaj.art-d0b5b86c3c99469097304319cec4b2c5 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-13T09:30:35Z |
| publishDate | 2014-02-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-d0b5b86c3c99469097304319cec4b2c52022-12-22T02:52:16ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982014-02-017181310.3980/j.issn.2222-3959.2014.01.02Effect of EGb761 on light-damaged retinal pigment epithelial cellsYun-Yun Zhou0Chang-Zheng Chen1Yu Su2Lu Li3Zuo-Hui-Zi Yi4Hang Qi5Ming Weng6Yi-Qiao Xing7Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China<br>Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China<br>Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, ChinaDepartment of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China<br>Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China<br>Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China<br>Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China<br>Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China<br><b>AIM:</b>To investigate the protective mechanism of Gingko Biloba extract (EGb761) on the ability of retinal pigment epithelial (RPE) cells to resist light-induced damage in a comparative proteomics study.<b>METHODS:</b>Human RPE cells (ARPE-19) were randomly distributed to one of three groups:normal control (NC group) and light-damaged model without or with EGb761 group (M and ME groups, respectively). The light-damaged model was formed by exposing to white light (2 200±300)lx for 6h. The RPE cells in ME group were conducted with EGb 761 (100μg/mL) before light exposure. The soluble cellular proteins extracting from each groups were separated by two-dimensional electrophoresis and stained by silver staining. Different proteins in the profiles of the gels were analyzed by Image Master Software. Two-fold expressing protein spots were identified by Matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry.<b>RESULTS:</b> NC, M and ME groups displayed 1 892±71, 2 145±23 and 2 216±85 protein spots, respectively. We identified 33 proteins with different expression levels between the NC and M groups, 25 proteins between the M and ME groups, and 11 proteins between the NC and ME groups. MALDI-TOF/TOF mass spectrometry successfully identified 16 proteins, including metabolic enzymes, cytoskeletal proteins, anti-oxidation proteins, and others.<b>CONCLUSION:</b>Differences in some important proteins, such as cathepsin B, heat shock protein, and cytochrome creductase, indicated that multiple pathways may be induced in light-damaged RPE cells and the protective effect of EGb761.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949451/EGb761retinal pigment epithelial cellslight damageproteinmass spectrographic analysismass spectrographic analysis |
| spellingShingle | Yun-Yun Zhou Chang-Zheng Chen Yu Su Lu Li Zuo-Hui-Zi Yi Hang Qi Ming Weng Yi-Qiao Xing Effect of EGb761 on light-damaged retinal pigment epithelial cells International Journal of Ophthalmology EGb761 retinal pigment epithelial cells light damage protein mass spectrographic analysis mass spectrographic analysis |
| title | Effect of EGb761 on light-damaged retinal pigment epithelial cells |
| title_full | Effect of EGb761 on light-damaged retinal pigment epithelial cells |
| title_fullStr | Effect of EGb761 on light-damaged retinal pigment epithelial cells |
| title_full_unstemmed | Effect of EGb761 on light-damaged retinal pigment epithelial cells |
| title_short | Effect of EGb761 on light-damaged retinal pigment epithelial cells |
| title_sort | effect of egb761 on light damaged retinal pigment epithelial cells |
| topic | EGb761 retinal pigment epithelial cells light damage protein mass spectrographic analysis mass spectrographic analysis |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949451/ |
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