In vitro PROPAGATION OF Arnica montana L.: AN ENDANGERED HERBAL SPECIES OF GREAT IMPORTANCE TO MEDICINE
Arnica montana L., a valuable medicinal plant, has been used in the pharmaceutical and cosmetic industry for many years. Traditional methods of reproduction of this protected species are hardly efficient; hence, the method of in vitro culture provides the possibility of increasing the multiplicatio...
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2012-04-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/3047 |
| _version_ | 1827587059155468288 |
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| author | Agnieszka Surmacz-Magdziak Danuta Sugier |
| author_facet | Agnieszka Surmacz-Magdziak Danuta Sugier |
| author_sort | Agnieszka Surmacz-Magdziak |
| collection | DOAJ |
| description |
Arnica montana L., a valuable medicinal plant, has been used in the pharmaceutical and cosmetic industry for many years. Traditional methods of reproduction of this protected species are hardly efficient; hence, the method of in vitro culture provides the possibility of increasing the multiplication coefficient, which in turn would facilitate introduction of arnica into crop production. The aim of the study was to examine the effect of cytokinins and their concentration on multiplication of shoots and the effect of different concentrations of auxin on rooting of multiplied shoots of Arnica montana. The plants of mountain arnica were propagated from shoot tips placed on the MS agar (0.7%) medium supplemented with NAA (0.1 mg ·l-1). One of the four cytokinins, i.e. BAP, KIN, 2iP and Z, was applied at the concentrations of 0.5, 1.0, 1.5 and 2.0 mg ·l-1. After 5 weeks of the in vitro culture, the shoots obtained were transferred to the MS agar medium supplemented
with 0, 0.05, 0.1 and 0.2 mg ·l-1 NAA for rooting. The greatest number of the explants regenerating shoots and the shoots number were found in the MS medium supplemented with 0.5 mg·l-1 BAP. KIN at the concentrations of 1.5 and 2.0 mg·l-1 exerted the most substantial impact on the shoot length. No change in shoot length and weight was found in the case of Z, and a bigger number of shoots was obtained at its concentrations ranging 1.0 to 2.0 mg·l-1. The shoots of the mountain arnica were characterised by high rhizogenic capacity. The biggest numbers of rooted shoots were found in the medium containing 0.1 mg·l-1 NAA. Plants with the greatest number and weight of shoots were obtained in the same medium, while the longest roots were found at 0.05 mg·l-1 NAA.
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| first_indexed | 2024-03-09T00:12:37Z |
| format | Article |
| id | doaj.art-d0b7d58fef2b465b8274b1c9fa356f71 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:12:37Z |
| publishDate | 2012-04-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-d0b7d58fef2b465b8274b1c9fa356f712023-12-12T09:56:25ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052012-04-01112In vitro PROPAGATION OF Arnica montana L.: AN ENDANGERED HERBAL SPECIES OF GREAT IMPORTANCE TO MEDICINEAgnieszka Surmacz-MagdziakDanuta Sugier Arnica montana L., a valuable medicinal plant, has been used in the pharmaceutical and cosmetic industry for many years. Traditional methods of reproduction of this protected species are hardly efficient; hence, the method of in vitro culture provides the possibility of increasing the multiplication coefficient, which in turn would facilitate introduction of arnica into crop production. The aim of the study was to examine the effect of cytokinins and their concentration on multiplication of shoots and the effect of different concentrations of auxin on rooting of multiplied shoots of Arnica montana. The plants of mountain arnica were propagated from shoot tips placed on the MS agar (0.7%) medium supplemented with NAA (0.1 mg ·l-1). One of the four cytokinins, i.e. BAP, KIN, 2iP and Z, was applied at the concentrations of 0.5, 1.0, 1.5 and 2.0 mg ·l-1. After 5 weeks of the in vitro culture, the shoots obtained were transferred to the MS agar medium supplemented with 0, 0.05, 0.1 and 0.2 mg ·l-1 NAA for rooting. The greatest number of the explants regenerating shoots and the shoots number were found in the MS medium supplemented with 0.5 mg·l-1 BAP. KIN at the concentrations of 1.5 and 2.0 mg·l-1 exerted the most substantial impact on the shoot length. No change in shoot length and weight was found in the case of Z, and a bigger number of shoots was obtained at its concentrations ranging 1.0 to 2.0 mg·l-1. The shoots of the mountain arnica were characterised by high rhizogenic capacity. The biggest numbers of rooted shoots were found in the medium containing 0.1 mg·l-1 NAA. Plants with the greatest number and weight of shoots were obtained in the same medium, while the longest roots were found at 0.05 mg·l-1 NAA. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/3047micropropagationmountain arnicagrowth regulators |
| spellingShingle | Agnieszka Surmacz-Magdziak Danuta Sugier In vitro PROPAGATION OF Arnica montana L.: AN ENDANGERED HERBAL SPECIES OF GREAT IMPORTANCE TO MEDICINE Acta Scientiarum Polonorum: Hortorum Cultus micropropagation mountain arnica growth regulators |
| title | In vitro PROPAGATION OF Arnica montana L.: AN ENDANGERED HERBAL SPECIES OF GREAT IMPORTANCE TO MEDICINE |
| title_full | In vitro PROPAGATION OF Arnica montana L.: AN ENDANGERED HERBAL SPECIES OF GREAT IMPORTANCE TO MEDICINE |
| title_fullStr | In vitro PROPAGATION OF Arnica montana L.: AN ENDANGERED HERBAL SPECIES OF GREAT IMPORTANCE TO MEDICINE |
| title_full_unstemmed | In vitro PROPAGATION OF Arnica montana L.: AN ENDANGERED HERBAL SPECIES OF GREAT IMPORTANCE TO MEDICINE |
| title_short | In vitro PROPAGATION OF Arnica montana L.: AN ENDANGERED HERBAL SPECIES OF GREAT IMPORTANCE TO MEDICINE |
| title_sort | in vitro propagation of arnica montana l an endangered herbal species of great importance to medicine |
| topic | micropropagation mountain arnica growth regulators |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/3047 |
| work_keys_str_mv | AT agnieszkasurmaczmagdziak invitropropagationofarnicamontanalanendangeredherbalspeciesofgreatimportancetomedicine AT danutasugier invitropropagationofarnicamontanalanendangeredherbalspeciesofgreatimportancetomedicine |