Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories
Abstract Background Nipah virus (NiV) is an emerging zoonotic paramyxovirus that causes severe encephalitis and respiratory disease with a high mortality rate in humans. During large outbreaks of the viral disease, serological testing of serum samples could be a useful diagnostic tool, which could p...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12985-020-01425-8 |
| _version_ | 1819226888643543040 |
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| author | Shumpei Watanabe Shuetsu Fukushi Toshihiko Harada Masayuki Shimojima Tomoki Yoshikawa Takeshi Kurosu Yoshihiro Kaku Shigeru Morikawa Masayuki Saijo |
| author_facet | Shumpei Watanabe Shuetsu Fukushi Toshihiko Harada Masayuki Shimojima Tomoki Yoshikawa Takeshi Kurosu Yoshihiro Kaku Shigeru Morikawa Masayuki Saijo |
| author_sort | Shumpei Watanabe |
| collection | DOAJ |
| description | Abstract Background Nipah virus (NiV) is an emerging zoonotic paramyxovirus that causes severe encephalitis and respiratory disease with a high mortality rate in humans. During large outbreaks of the viral disease, serological testing of serum samples could be a useful diagnostic tool, which could provide information on not only the diagnosis of NiV disease but also the history of an individual with previous exposure to the virus, thereby supporting disease control. Therefore, an efficient method for the inactivation of NiV in serum samples is required for serological diagnosis. Methods We determined the optimal conditions for the inactivation of NiV infectivity in human serum using heating and UV treatment. The inactivation method comprised UV irradiation with a cover of aluminum foil for 30 min and heating at 56 °C for 30 min. Results With an optimized protocol for virus inactivation, NiV infectivity in serum samples (containing 6.0 × 105 TCID50) was completely inactivated. Conclusions We developed a recommended protocol for the effective inactivation of NiV. This protocol would enable a regional or local laboratory to safely transport or process samples, including NiV, for serological testing in its biosafety level-2 facility. |
| first_indexed | 2024-12-23T10:32:39Z |
| format | Article |
| id | doaj.art-d0ba60568cf4418dbded396f0776e6d2 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-23T10:32:39Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-d0ba60568cf4418dbded396f0776e6d22022-12-21T17:50:24ZengBMCVirology Journal1743-422X2020-10-011711810.1186/s12985-020-01425-8Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratoriesShumpei Watanabe0Shuetsu Fukushi1Toshihiko Harada2Masayuki Shimojima3Tomoki Yoshikawa4Takeshi Kurosu5Yoshihiro Kaku6Shigeru Morikawa7Masayuki Saijo8Department of Microbiology, Faculty of Veterinary Medicine, Okayama University of ScienceDepartment of Virology I, National Institute of Infectious DiseasesManagement Department of Biosafety and Laboratory Animal, National Institute of Infectious DiseasesDepartment of Virology I, National Institute of Infectious DiseasesDepartment of Virology I, National Institute of Infectious DiseasesDepartment of Virology I, National Institute of Infectious DiseasesDivision of Veterinary Science, National Institute of Infectious DiseasesDepartment of Microbiology, Faculty of Veterinary Medicine, Okayama University of ScienceDepartment of Virology I, National Institute of Infectious DiseasesAbstract Background Nipah virus (NiV) is an emerging zoonotic paramyxovirus that causes severe encephalitis and respiratory disease with a high mortality rate in humans. During large outbreaks of the viral disease, serological testing of serum samples could be a useful diagnostic tool, which could provide information on not only the diagnosis of NiV disease but also the history of an individual with previous exposure to the virus, thereby supporting disease control. Therefore, an efficient method for the inactivation of NiV in serum samples is required for serological diagnosis. Methods We determined the optimal conditions for the inactivation of NiV infectivity in human serum using heating and UV treatment. The inactivation method comprised UV irradiation with a cover of aluminum foil for 30 min and heating at 56 °C for 30 min. Results With an optimized protocol for virus inactivation, NiV infectivity in serum samples (containing 6.0 × 105 TCID50) was completely inactivated. Conclusions We developed a recommended protocol for the effective inactivation of NiV. This protocol would enable a regional or local laboratory to safely transport or process samples, including NiV, for serological testing in its biosafety level-2 facility.http://link.springer.com/article/10.1186/s12985-020-01425-8Nipah virusVirus inactivationDiagnosisBiosafety level 4Virus stability |
| spellingShingle | Shumpei Watanabe Shuetsu Fukushi Toshihiko Harada Masayuki Shimojima Tomoki Yoshikawa Takeshi Kurosu Yoshihiro Kaku Shigeru Morikawa Masayuki Saijo Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories Virology Journal Nipah virus Virus inactivation Diagnosis Biosafety level 4 Virus stability |
| title | Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories |
| title_full | Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories |
| title_fullStr | Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories |
| title_full_unstemmed | Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories |
| title_short | Effective inactivation of Nipah virus in serum samples for safe processing in low-containment laboratories |
| title_sort | effective inactivation of nipah virus in serum samples for safe processing in low containment laboratories |
| topic | Nipah virus Virus inactivation Diagnosis Biosafety level 4 Virus stability |
| url | http://link.springer.com/article/10.1186/s12985-020-01425-8 |
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