Screening and Characterization of <i>Pediococcus acidilactici</i> LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties
Growing interest has been focused on lactic acid bacteria as alternatives to antimicrobial growth promoters, which are characterized by the production of various functional metabolites, such as antimicrobial and antioxidants compounds. The present study was undertaken to evaluate a potential probiot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/2/215 |
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| author | Chong Li Shaolong Wang Si Chen Xiaoying Wang Xuejuan Deng Guohua Liu Wenhuan Chang Yves Beckers Huiyi Cai |
| author_facet | Chong Li Shaolong Wang Si Chen Xiaoying Wang Xuejuan Deng Guohua Liu Wenhuan Chang Yves Beckers Huiyi Cai |
| author_sort | Chong Li |
| collection | DOAJ |
| description | Growing interest has been focused on lactic acid bacteria as alternatives to antimicrobial growth promoters, which are characterized by the production of various functional metabolites, such as antimicrobial and antioxidants compounds. The present study was undertaken to evaluate a potential probiotic from the antioxidant perspective. LC-9-1, screened from the intestines of healthy animals, was revealed to be <i>Pediococcus acidilactici</i> on the basis of its morphological, biochemical, and molecular characteristics. The strain has excellent properties, including acid-production efficiency, antibacterial performance and antioxidant activity. The safety of the strain was also evaluated. Furthermore, the experiments in broiler chickens suggested that dietary LC-9-1 supplementation improved the growth performance and decreased the abdominal fat, and enhanced the antioxidant capability and intestinal innate immunity of broilers. Analysis of intestinal microbiota showed that a higher community diversity (Shannon index) was achieved. In addition to the significantly increased relative abundances of <i>Pediococcus</i> spp., beneficial genera such as <i>Rothia</i> spp. and <i>Ruminococcus</i> spp. were abundant, while opportunistic pathogens such as <i>Escherichia-Shigella</i> spp. were significantly reduced in LC-9-1-supplemented broilers. Collectively, such in-depth characterization and the available data will guide future efforts to develop next-generation probiotics, and LC-9-1 could be considered a potential strain for further utilization in direct-fed microbial or starter culture for fermentation. |
| first_indexed | 2024-03-11T09:15:23Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T09:15:23Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d0bb10c3b4664993a56bec655d90908e2023-11-16T18:44:58ZengMDPI AGAntioxidants2076-39212023-01-0112221510.3390/antiox12020215Screening and Characterization of <i>Pediococcus acidilactici</i> LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative PropertiesChong Li0Shaolong Wang1Si Chen2Xiaoying Wang3Xuejuan Deng4Guohua Liu5Wenhuan Chang6Yves Beckers7Huiyi Cai8Key Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaDepartment of Molecular Cell Biology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of KoreaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaNational Engineering Research Center of Biological Feed, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaPrecision Livestock and Nutrition Laboratory, Teaching and Research Centre (TERRA), Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, BelgiumKey Laboratory for Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agriculture Sciences, Beijing 100081, ChinaGrowing interest has been focused on lactic acid bacteria as alternatives to antimicrobial growth promoters, which are characterized by the production of various functional metabolites, such as antimicrobial and antioxidants compounds. The present study was undertaken to evaluate a potential probiotic from the antioxidant perspective. LC-9-1, screened from the intestines of healthy animals, was revealed to be <i>Pediococcus acidilactici</i> on the basis of its morphological, biochemical, and molecular characteristics. The strain has excellent properties, including acid-production efficiency, antibacterial performance and antioxidant activity. The safety of the strain was also evaluated. Furthermore, the experiments in broiler chickens suggested that dietary LC-9-1 supplementation improved the growth performance and decreased the abdominal fat, and enhanced the antioxidant capability and intestinal innate immunity of broilers. Analysis of intestinal microbiota showed that a higher community diversity (Shannon index) was achieved. In addition to the significantly increased relative abundances of <i>Pediococcus</i> spp., beneficial genera such as <i>Rothia</i> spp. and <i>Ruminococcus</i> spp. were abundant, while opportunistic pathogens such as <i>Escherichia-Shigella</i> spp. were significantly reduced in LC-9-1-supplemented broilers. Collectively, such in-depth characterization and the available data will guide future efforts to develop next-generation probiotics, and LC-9-1 could be considered a potential strain for further utilization in direct-fed microbial or starter culture for fermentation.https://www.mdpi.com/2076-3921/12/2/215<i>Pediococcus acidilactici</i>screeningantibacterialantioxidantbroiler chickensintestinal microbiota |
| spellingShingle | Chong Li Shaolong Wang Si Chen Xiaoying Wang Xuejuan Deng Guohua Liu Wenhuan Chang Yves Beckers Huiyi Cai Screening and Characterization of <i>Pediococcus acidilactici</i> LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties Antioxidants <i>Pediococcus acidilactici</i> screening antibacterial antioxidant broiler chickens intestinal microbiota |
| title | Screening and Characterization of <i>Pediococcus acidilactici</i> LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties |
| title_full | Screening and Characterization of <i>Pediococcus acidilactici</i> LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties |
| title_fullStr | Screening and Characterization of <i>Pediococcus acidilactici</i> LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties |
| title_full_unstemmed | Screening and Characterization of <i>Pediococcus acidilactici</i> LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties |
| title_short | Screening and Characterization of <i>Pediococcus acidilactici</i> LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties |
| title_sort | screening and characterization of i pediococcus acidilactici i lc 9 1 toward selection as a potential probiotic for poultry with antibacterial and antioxidative properties |
| topic | <i>Pediococcus acidilactici</i> screening antibacterial antioxidant broiler chickens intestinal microbiota |
| url | https://www.mdpi.com/2076-3921/12/2/215 |
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