Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol.
The interaction of sonicated, small vesicles of egg phosphatidylcholine and cholesterol (2:1, mol/mol) with bovine high density serum lipoproteins was examined in terms of lipid transfer between both types of particles and the resulting changes in lipoprotein structure. Saturation of high density li...
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| Format: | Article |
| Language: | English |
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Elsevier
1979-09-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520400100 |
| _version_ | 1818459717304844288 |
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| author | A Jonas |
| author_facet | A Jonas |
| author_sort | A Jonas |
| collection | DOAJ |
| description | The interaction of sonicated, small vesicles of egg phosphatidylcholine and cholesterol (2:1, mol/mol) with bovine high density serum lipoproteins was examined in terms of lipid transfer between both types of particles and the resulting changes in lipoprotein structure. Saturation of high density lipoprotein preparations with vesicle lipids gave final lipoprotein particles with essentially unchanged protein content and composition, unchanged cholesterylester and nonpolar lipid content, but with markedly increased phospholipid content (59% increas by weight) and moderately increased cholesterol content (20% increase by weight). The lipoproteins enriched in lipid were relatively uniform, spherical particles, 110 +/- 3.6 A in diameter (6 A larger than the original lipoproteins); they had a markedly decreased intrinsic protein fluorescence, a red-shifted fluorescence wavelength maximum, and more fluid lipid domains. These results indicate that the direct addition of excess lipids from membranes or other lipoproteins is a possible mechanism for lipid transfer to high density lipoproteins. Also they suggest a structural flexibility of high density lipoproteins that allows the addition of significant amounts of surface components. |
| first_indexed | 2024-12-14T23:18:47Z |
| format | Article |
| id | doaj.art-d0bcfa760dab477890c072aa7a845850 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T23:18:47Z |
| publishDate | 1979-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-d0bcfa760dab477890c072aa7a8458502022-12-21T22:44:01ZengElsevierJournal of Lipid Research0022-22751979-09-01207817824Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol.A JonasThe interaction of sonicated, small vesicles of egg phosphatidylcholine and cholesterol (2:1, mol/mol) with bovine high density serum lipoproteins was examined in terms of lipid transfer between both types of particles and the resulting changes in lipoprotein structure. Saturation of high density lipoprotein preparations with vesicle lipids gave final lipoprotein particles with essentially unchanged protein content and composition, unchanged cholesterylester and nonpolar lipid content, but with markedly increased phospholipid content (59% increas by weight) and moderately increased cholesterol content (20% increase by weight). The lipoproteins enriched in lipid were relatively uniform, spherical particles, 110 +/- 3.6 A in diameter (6 A larger than the original lipoproteins); they had a markedly decreased intrinsic protein fluorescence, a red-shifted fluorescence wavelength maximum, and more fluid lipid domains. These results indicate that the direct addition of excess lipids from membranes or other lipoproteins is a possible mechanism for lipid transfer to high density lipoproteins. Also they suggest a structural flexibility of high density lipoproteins that allows the addition of significant amounts of surface components.http://www.sciencedirect.com/science/article/pii/S0022227520400100 |
| spellingShingle | A Jonas Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol. Journal of Lipid Research |
| title | Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol. |
| title_full | Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol. |
| title_fullStr | Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol. |
| title_full_unstemmed | Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol. |
| title_short | Interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol. |
| title_sort | interaction of bovine serum high density lipoprotein with mixed vesicles of phosphatidylcholine and cholesterol |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520400100 |
| work_keys_str_mv | AT ajonas interactionofbovineserumhighdensitylipoproteinwithmixedvesiclesofphosphatidylcholineandcholesterol |