Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine
Background/Aims: Circulating miRNAs could serve as biomarkers for diagnosis or prognosis of heart diseases and cerebrovascular diseases. Dexmedetomidine has protective effects in various organs. The effects of dexmedetomidine on circulating miRNAs remain unknown. Here, we investigated differentially...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2018-10-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | https://www.karger.com/Article/FullText/494168 |
| _version_ | 1811336725160525824 |
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| author | Xuehui Yang Hongmei Chen Yan Chen Yochai Birnbaum Rongbi Liang Yumei Ye Jinqiao Qian |
| author_facet | Xuehui Yang Hongmei Chen Yan Chen Yochai Birnbaum Rongbi Liang Yumei Ye Jinqiao Qian |
| author_sort | Xuehui Yang |
| collection | DOAJ |
| description | Background/Aims: Circulating miRNAs could serve as biomarkers for diagnosis or prognosis of heart diseases and cerebrovascular diseases. Dexmedetomidine has protective effects in various organs. The effects of dexmedetomidine on circulating miRNAs remain unknown. Here, we investigated differentially expressed miRNA and to predict the target genes of the miRNA in patients receiving dexmedetomidine. Methods: The expression levels of circulating miRNAs of 3 patients were determined through high through-put miRNA sequencing technology. Target genes of the identified differentially expressed miRNAs were predicted using TargetScan 7.1 and miRDB v.5. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional annotation and pathway enrichment analysis of target genes respectively. Results: Twelve differentially expressed miRNAs were identified. Five miRNAs were upregulated (hsa-miR-4508, hsa-miR-novel-chr8_87373, hsa-miR-30a-3p, hsa-miR-novel-chr16_26099, hsa-miR-4306) and seven miRNAs (hsa-miR-744-5p, hsa-miR-320a, hsa-miR-novel-chr9_90035, hsa-miR-101-3p, hsa-miR-150-5p, hsa-miR-342-3p, and hsa-miR-140-3p) were downregulated after administration of dexmedetomidine in the subjects. The target genes and pathways related to the differentially expressed miRNAs were predicted and analyzed. Conclusion: The differentially expressed miRNAs may be involved in the mechanisms of action of dexmedetomidine. Specific miRNAs, such as hsa-miR-101-3p, hsa-miR-150-5p and hsa-miR-140-3p, are new potential targets for further functional studies of dexmedetomidine. |
| first_indexed | 2024-04-13T17:43:41Z |
| format | Article |
| id | doaj.art-d0be67d94188414e937ed2c3af772d43 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-04-13T17:43:41Z |
| publishDate | 2018-10-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-d0be67d94188414e937ed2c3af772d432022-12-22T02:37:06ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-10-0150255256810.1159/000494168494168Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving DexmedetomidineXuehui YangHongmei ChenYan ChenYochai BirnbaumRongbi LiangYumei YeJinqiao QianBackground/Aims: Circulating miRNAs could serve as biomarkers for diagnosis or prognosis of heart diseases and cerebrovascular diseases. Dexmedetomidine has protective effects in various organs. The effects of dexmedetomidine on circulating miRNAs remain unknown. Here, we investigated differentially expressed miRNA and to predict the target genes of the miRNA in patients receiving dexmedetomidine. Methods: The expression levels of circulating miRNAs of 3 patients were determined through high through-put miRNA sequencing technology. Target genes of the identified differentially expressed miRNAs were predicted using TargetScan 7.1 and miRDB v.5. Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional annotation and pathway enrichment analysis of target genes respectively. Results: Twelve differentially expressed miRNAs were identified. Five miRNAs were upregulated (hsa-miR-4508, hsa-miR-novel-chr8_87373, hsa-miR-30a-3p, hsa-miR-novel-chr16_26099, hsa-miR-4306) and seven miRNAs (hsa-miR-744-5p, hsa-miR-320a, hsa-miR-novel-chr9_90035, hsa-miR-101-3p, hsa-miR-150-5p, hsa-miR-342-3p, and hsa-miR-140-3p) were downregulated after administration of dexmedetomidine in the subjects. The target genes and pathways related to the differentially expressed miRNAs were predicted and analyzed. Conclusion: The differentially expressed miRNAs may be involved in the mechanisms of action of dexmedetomidine. Specific miRNAs, such as hsa-miR-101-3p, hsa-miR-150-5p and hsa-miR-140-3p, are new potential targets for further functional studies of dexmedetomidine.https://www.karger.com/Article/FullText/494168DexmedetomidineCirculating miRNAsExpression profilingIschemia/reperfusion injuryCardioprotection |
| spellingShingle | Xuehui Yang Hongmei Chen Yan Chen Yochai Birnbaum Rongbi Liang Yumei Ye Jinqiao Qian Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine Cellular Physiology and Biochemistry Dexmedetomidine Circulating miRNAs Expression profiling Ischemia/reperfusion injury Cardioprotection |
| title | Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine |
| title_full | Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine |
| title_fullStr | Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine |
| title_full_unstemmed | Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine |
| title_short | Circulating miRNA Expression Profiling and Target Prediction in Patients Receiving Dexmedetomidine |
| title_sort | circulating mirna expression profiling and target prediction in patients receiving dexmedetomidine |
| topic | Dexmedetomidine Circulating miRNAs Expression profiling Ischemia/reperfusion injury Cardioprotection |
| url | https://www.karger.com/Article/FullText/494168 |
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