Recombination in West Nile Virus: minimal contribution to genomic diversity
<p>Abstract</p> <p>Recombination is known to play a role in the ability of various viruses to acquire sequence diversity. We consequently examined all available West Nile virus (WNV) whole genome sequences both phylogenetically and with a variety of computational recombination dete...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2009-10-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/6/1/165 |
| _version_ | 1828359308003246080 |
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| author | Lefkowitz Elliot J Pickett Brett E |
| author_facet | Lefkowitz Elliot J Pickett Brett E |
| author_sort | Lefkowitz Elliot J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Recombination is known to play a role in the ability of various viruses to acquire sequence diversity. We consequently examined all available West Nile virus (WNV) whole genome sequences both phylogenetically and with a variety of computational recombination detection algorithms. We found that the number of distinct lineages present on a phylogenetic tree reconstruction to be identical to the 6 previously reported. Statistically-significant evidence for recombination was only observed in one whole genome sequence. This recombination event was within the NS5 polymerase coding region. All three viruses contributing to the recombination event were originally isolated in Africa at various times, with the major parent (SPU116_89_B), minor parent (KN3829), and recombinant sequence (AnMg798) belonging to WNV taxonomic lineages 2, 1a, and 2 respectively. This one isolated recombinant genome was out of a total of 154 sequences analyzed. It therefore does not seem likely that recombination contributes in any significant manner to the overall sequence variation within the WNV genome.</p> |
| first_indexed | 2024-04-14T03:39:56Z |
| format | Article |
| id | doaj.art-d0c53343ca814de393145ca848dfe3ed |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-14T03:39:56Z |
| publishDate | 2009-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-d0c53343ca814de393145ca848dfe3ed2022-12-22T02:14:34ZengBMCVirology Journal1743-422X2009-10-016116510.1186/1743-422X-6-165Recombination in West Nile Virus: minimal contribution to genomic diversityLefkowitz Elliot JPickett Brett E<p>Abstract</p> <p>Recombination is known to play a role in the ability of various viruses to acquire sequence diversity. We consequently examined all available West Nile virus (WNV) whole genome sequences both phylogenetically and with a variety of computational recombination detection algorithms. We found that the number of distinct lineages present on a phylogenetic tree reconstruction to be identical to the 6 previously reported. Statistically-significant evidence for recombination was only observed in one whole genome sequence. This recombination event was within the NS5 polymerase coding region. All three viruses contributing to the recombination event were originally isolated in Africa at various times, with the major parent (SPU116_89_B), minor parent (KN3829), and recombinant sequence (AnMg798) belonging to WNV taxonomic lineages 2, 1a, and 2 respectively. This one isolated recombinant genome was out of a total of 154 sequences analyzed. It therefore does not seem likely that recombination contributes in any significant manner to the overall sequence variation within the WNV genome.</p>http://www.virologyj.com/content/6/1/165 |
| spellingShingle | Lefkowitz Elliot J Pickett Brett E Recombination in West Nile Virus: minimal contribution to genomic diversity Virology Journal |
| title | Recombination in West Nile Virus: minimal contribution to genomic diversity |
| title_full | Recombination in West Nile Virus: minimal contribution to genomic diversity |
| title_fullStr | Recombination in West Nile Virus: minimal contribution to genomic diversity |
| title_full_unstemmed | Recombination in West Nile Virus: minimal contribution to genomic diversity |
| title_short | Recombination in West Nile Virus: minimal contribution to genomic diversity |
| title_sort | recombination in west nile virus minimal contribution to genomic diversity |
| url | http://www.virologyj.com/content/6/1/165 |
| work_keys_str_mv | AT lefkowitzelliotj recombinationinwestnilevirusminimalcontributiontogenomicdiversity AT pickettbrette recombinationinwestnilevirusminimalcontributiontogenomicdiversity |