Effects of nicotinamide on follicular development and the quality of oocytes
Abstract Background Nicotinamide (NAM) is an important antioxidant, which is closely related to female fertility, but its role has not been clearly elucidated. The purpose of the present study was to investigate the effects of NAM on follicular development at different stages and the quality of oocy...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12958-022-00938-x |
| _version_ | 1828414911997280256 |
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| author | Ziyu Guo Jihong Yang Guangping Yang Ting Feng Xinyue Zhang Yao Chen Ruizhi Feng Yun Qian |
| author_facet | Ziyu Guo Jihong Yang Guangping Yang Ting Feng Xinyue Zhang Yao Chen Ruizhi Feng Yun Qian |
| author_sort | Ziyu Guo |
| collection | DOAJ |
| description | Abstract Background Nicotinamide (NAM) is an important antioxidant, which is closely related to female fertility, but its role has not been clearly elucidated. The purpose of the present study was to investigate the effects of NAM on follicular development at different stages and the quality of oocytes. Methods The concentration of NAM in follicular fluid (FF) of 236 women undergoing in vitro fertilization (IVF) was ascertained by enzyme-linked immunosorbent assay (ELISA), and the correlation between NAM and clinical indexes was analyzed. During the in vitro maturation (IVM) of mice cumulus-oocyte complexes (COCs), different concentrations of NAM were added to check the maturation rate and fertilization rate. The reactive oxygen species (ROS) levels in the oocytes treated with different hydrogen peroxide (H2O2) and NAM were assessed. Immunofluorescence staining was performed to measure the proportion of abnormal spindles. Results The level of NAM in large follicles was significantly higher than that in small follicles. In mature FF, the NAM concentration was positively correlated with the rates of oocyte maturation and fertilization. Five mM NAM treatment during IVM increased maturation rate and fertilization rate in the oxidative stress model, and significantly reduced the increase of ROS levels induced by H2O2 in mice oocytes. Conclusions Higher levels of NAM in FF are associated with larger follicle development. The supplement of 5 mM NAM during IVM may improve mice oocyte quality, reducing damage caused by oxidative stress. |
| first_indexed | 2024-12-10T13:35:11Z |
| format | Article |
| id | doaj.art-d0ca5d6ce2e947249092f719b15a3161 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-10T13:35:11Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-d0ca5d6ce2e947249092f719b15a31612022-12-22T01:46:51ZengBMCReproductive Biology and Endocrinology1477-78272022-04-0120111210.1186/s12958-022-00938-xEffects of nicotinamide on follicular development and the quality of oocytesZiyu Guo0Jihong Yang1Guangping Yang2Ting Feng3Xinyue Zhang4Yao Chen5Ruizhi Feng6Yun Qian7Reproductive Center of Second Affiliated Hospital of Nanjing Medical UniversityReproductive Center of Second Affiliated Hospital of Nanjing Medical UniversityState Key Laboratory of Reproductive Medicine, Nanjing Medical UniversityReproductive Center of Second Affiliated Hospital of Nanjing Medical UniversityReproductive Center of Second Affiliated Hospital of Nanjing Medical UniversityReproductive Center of Second Affiliated Hospital of Nanjing Medical UniversityState Key Laboratory of Reproductive Medicine, Nanjing Medical UniversityReproductive Center of Second Affiliated Hospital of Nanjing Medical UniversityAbstract Background Nicotinamide (NAM) is an important antioxidant, which is closely related to female fertility, but its role has not been clearly elucidated. The purpose of the present study was to investigate the effects of NAM on follicular development at different stages and the quality of oocytes. Methods The concentration of NAM in follicular fluid (FF) of 236 women undergoing in vitro fertilization (IVF) was ascertained by enzyme-linked immunosorbent assay (ELISA), and the correlation between NAM and clinical indexes was analyzed. During the in vitro maturation (IVM) of mice cumulus-oocyte complexes (COCs), different concentrations of NAM were added to check the maturation rate and fertilization rate. The reactive oxygen species (ROS) levels in the oocytes treated with different hydrogen peroxide (H2O2) and NAM were assessed. Immunofluorescence staining was performed to measure the proportion of abnormal spindles. Results The level of NAM in large follicles was significantly higher than that in small follicles. In mature FF, the NAM concentration was positively correlated with the rates of oocyte maturation and fertilization. Five mM NAM treatment during IVM increased maturation rate and fertilization rate in the oxidative stress model, and significantly reduced the increase of ROS levels induced by H2O2 in mice oocytes. Conclusions Higher levels of NAM in FF are associated with larger follicle development. The supplement of 5 mM NAM during IVM may improve mice oocyte quality, reducing damage caused by oxidative stress.https://doi.org/10.1186/s12958-022-00938-xFemale infertilityNicotinamideFollicular fluidFollicle sizeOocyte qualityOxidative stress |
| spellingShingle | Ziyu Guo Jihong Yang Guangping Yang Ting Feng Xinyue Zhang Yao Chen Ruizhi Feng Yun Qian Effects of nicotinamide on follicular development and the quality of oocytes Reproductive Biology and Endocrinology Female infertility Nicotinamide Follicular fluid Follicle size Oocyte quality Oxidative stress |
| title | Effects of nicotinamide on follicular development and the quality of oocytes |
| title_full | Effects of nicotinamide on follicular development and the quality of oocytes |
| title_fullStr | Effects of nicotinamide on follicular development and the quality of oocytes |
| title_full_unstemmed | Effects of nicotinamide on follicular development and the quality of oocytes |
| title_short | Effects of nicotinamide on follicular development and the quality of oocytes |
| title_sort | effects of nicotinamide on follicular development and the quality of oocytes |
| topic | Female infertility Nicotinamide Follicular fluid Follicle size Oocyte quality Oxidative stress |
| url | https://doi.org/10.1186/s12958-022-00938-x |
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