GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite <i>Coptotermes formosanus</i> Shiraki Digesting the Weed <i>Mikania micrantha</i> Kunth
Effective approaches to exploiting the biomass of the abundant invasive weed <i>Mikania micrantha</i> Kunth are limited. Termites have been a focus of significant attention as mediators of biomass-processing owing to their ability to digest lignocellulose. Here, the GC/TOF-MS approach wa...
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MDPI AG
2021-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/10/927 |
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| author | Wenjing Wu Yahui Hou Shijun Zhang Yong Chen Wenhui Zeng Zhiqiang Li |
| author_facet | Wenjing Wu Yahui Hou Shijun Zhang Yong Chen Wenhui Zeng Zhiqiang Li |
| author_sort | Wenjing Wu |
| collection | DOAJ |
| description | Effective approaches to exploiting the biomass of the abundant invasive weed <i>Mikania micrantha</i> Kunth are limited. Termites have been a focus of significant attention as mediators of biomass-processing owing to their ability to digest lignocellulose. Here, the GC/TOF-MS approach was employed to assess the effects of a diet composed of <i>M. micrantha</i> leaves on <i>Coptotermes formosanus</i> workers, with the growth performance of these workers also being assessed. The workers increased their dietary intake when fed <i>M. micrantha</i> leaves, with a concomitant gradual increase in mortality rate. A total of 62 differentially abundant metabolites and nine significantly affected pathways were found when comparing termites fed <i>M. micrantha</i> leaves to pinewood. Key metabolites, including carbohydrates, polyols, 4-hydroxyphenylacetic acid, and their related metabolic pathways, suggested that termites can digest and utilize <i>M. micrantha</i>-derived lignocellulose. However, changes in the tryptophan metabolism, tyrosine metabolism, and sphingolipid metabolism suggest an adverse effect of <i>M. micrantha</i> leaves on antioxidant activity and signal transduction in termites. Overall, this study identified the key metabolites and pathways associated with the response of these termites to dietary changes and the effect of <i>M. micrantha</i> on termites. |
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| language | English |
| last_indexed | 2024-03-10T06:29:16Z |
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| series | Insects |
| spelling | doaj.art-d0cc2f1ff2794fa9ad8414d4fc7214c62023-11-22T18:39:47ZengMDPI AGInsects2075-44502021-10-01121092710.3390/insects12100927GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite <i>Coptotermes formosanus</i> Shiraki Digesting the Weed <i>Mikania micrantha</i> KunthWenjing Wu0Yahui Hou1Shijun Zhang2Yong Chen3Wenhui Zeng4Zhiqiang Li5Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510405, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, ChinaEffective approaches to exploiting the biomass of the abundant invasive weed <i>Mikania micrantha</i> Kunth are limited. Termites have been a focus of significant attention as mediators of biomass-processing owing to their ability to digest lignocellulose. Here, the GC/TOF-MS approach was employed to assess the effects of a diet composed of <i>M. micrantha</i> leaves on <i>Coptotermes formosanus</i> workers, with the growth performance of these workers also being assessed. The workers increased their dietary intake when fed <i>M. micrantha</i> leaves, with a concomitant gradual increase in mortality rate. A total of 62 differentially abundant metabolites and nine significantly affected pathways were found when comparing termites fed <i>M. micrantha</i> leaves to pinewood. Key metabolites, including carbohydrates, polyols, 4-hydroxyphenylacetic acid, and their related metabolic pathways, suggested that termites can digest and utilize <i>M. micrantha</i>-derived lignocellulose. However, changes in the tryptophan metabolism, tyrosine metabolism, and sphingolipid metabolism suggest an adverse effect of <i>M. micrantha</i> leaves on antioxidant activity and signal transduction in termites. Overall, this study identified the key metabolites and pathways associated with the response of these termites to dietary changes and the effect of <i>M. micrantha</i> on termites.https://www.mdpi.com/2075-4450/12/10/927<i>Coptotermes formosanus</i><i>Mikania micrantha</i>gas chromatography/time-of-flight mass spectrometry (GC/TOF-MS)metabolic profilesdietary intakemortality |
| spellingShingle | Wenjing Wu Yahui Hou Shijun Zhang Yong Chen Wenhui Zeng Zhiqiang Li GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite <i>Coptotermes formosanus</i> Shiraki Digesting the Weed <i>Mikania micrantha</i> Kunth Insects <i>Coptotermes formosanus</i> <i>Mikania micrantha</i> gas chromatography/time-of-flight mass spectrometry (GC/TOF-MS) metabolic profiles dietary intake mortality |
| title | GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite <i>Coptotermes formosanus</i> Shiraki Digesting the Weed <i>Mikania micrantha</i> Kunth |
| title_full | GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite <i>Coptotermes formosanus</i> Shiraki Digesting the Weed <i>Mikania micrantha</i> Kunth |
| title_fullStr | GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite <i>Coptotermes formosanus</i> Shiraki Digesting the Weed <i>Mikania micrantha</i> Kunth |
| title_full_unstemmed | GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite <i>Coptotermes formosanus</i> Shiraki Digesting the Weed <i>Mikania micrantha</i> Kunth |
| title_short | GC/TOF-MS-Based Metabolomics Reveals Altered Metabolic Profiles in Wood-Feeding Termite <i>Coptotermes formosanus</i> Shiraki Digesting the Weed <i>Mikania micrantha</i> Kunth |
| title_sort | gc tof ms based metabolomics reveals altered metabolic profiles in wood feeding termite i coptotermes formosanus i shiraki digesting the weed i mikania micrantha i kunth |
| topic | <i>Coptotermes formosanus</i> <i>Mikania micrantha</i> gas chromatography/time-of-flight mass spectrometry (GC/TOF-MS) metabolic profiles dietary intake mortality |
| url | https://www.mdpi.com/2075-4450/12/10/927 |
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