Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening
This study was designed to explore new antioxidant and antimicrobial agents from the methanol whole plant crude extract and fractions of Plantago rugelii. The methanol extract and its fractions were prepared and screened for its phytochemical composition, in-vitro antioxidant potential and challeng...
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| Format: | Article |
| Language: | English |
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Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)
2018-06-01
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| Series: | Journal of Applied Sciences and Environmental Management |
| Subjects: | |
| Online Access: | https://www.ajol.info/index.php/jasem/article/view/172634 |
| _version_ | 1797227795350290432 |
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| author | C Ogbiko CJ Eboka AD Tambuwal |
| author_facet | C Ogbiko CJ Eboka AD Tambuwal |
| author_sort | C Ogbiko |
| collection | DOAJ |
| description |
This study was designed to explore new antioxidant and antimicrobial agents from the methanol whole plant crude extract and fractions of Plantago rugelii. The methanol extract and its fractions were prepared and screened for its phytochemical composition, in-vitro antioxidant potential and challenged with common pathogenic microorganisms for its antimicrobial activities using standard procedures. The phytochemical analysis revealed the presence of various pharmaceutically active secondary metabolites like alkaloids, phenolic, flavonoids, carbohydrates, glycosides, sterols, etc. In the DPPH assay, the aqueous methanol fraction was found to be the most effective among all the fractions in comparison to the ascorbic acid standard. Using the zone of inhibition as inhibitory parameter, the crude methanol extract exhibited the best antibacterial activity when challenged against all the clinical isolates except Klebsiella pneumoniae. The aqueous methanol extract exhibited the best percentage fungal inhibition when compared to other fractions. The standard drugs ciprofloxacin and fluconazole exhibited a near 100% activity except with Proteus vulgaris where the crude methanol extract has a higher value.This research holds promise for the exploration of various potentially active secondary metabolites which would help in developing pharmaceuticals, especially antioxidant and antimicrobial drugs. The isolation and characterization of the exact metabolites responsible for these activities is therefore recommended.
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| first_indexed | 2024-04-24T14:46:29Z |
| format | Article |
| id | doaj.art-d0cd6ec085784c488ec8c215a89730e8 |
| institution | Directory Open Access Journal |
| issn | 2659-1502 2659-1499 |
| language | English |
| last_indexed | 2024-04-24T14:46:29Z |
| publishDate | 2018-06-01 |
| publisher | Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) |
| record_format | Article |
| series | Journal of Applied Sciences and Environmental Management |
| spelling | doaj.art-d0cd6ec085784c488ec8c215a89730e82024-04-02T19:55:44ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992018-06-0122510.4314/jasem.v22i5.1Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical ScreeningC OgbikoCJ EbokaAD Tambuwal This study was designed to explore new antioxidant and antimicrobial agents from the methanol whole plant crude extract and fractions of Plantago rugelii. The methanol extract and its fractions were prepared and screened for its phytochemical composition, in-vitro antioxidant potential and challenged with common pathogenic microorganisms for its antimicrobial activities using standard procedures. The phytochemical analysis revealed the presence of various pharmaceutically active secondary metabolites like alkaloids, phenolic, flavonoids, carbohydrates, glycosides, sterols, etc. In the DPPH assay, the aqueous methanol fraction was found to be the most effective among all the fractions in comparison to the ascorbic acid standard. Using the zone of inhibition as inhibitory parameter, the crude methanol extract exhibited the best antibacterial activity when challenged against all the clinical isolates except Klebsiella pneumoniae. The aqueous methanol extract exhibited the best percentage fungal inhibition when compared to other fractions. The standard drugs ciprofloxacin and fluconazole exhibited a near 100% activity except with Proteus vulgaris where the crude methanol extract has a higher value.This research holds promise for the exploration of various potentially active secondary metabolites which would help in developing pharmaceuticals, especially antioxidant and antimicrobial drugs. The isolation and characterization of the exact metabolites responsible for these activities is therefore recommended. https://www.ajol.info/index.php/jasem/article/view/172634Plantago rugeliiAntioxidantFree RadicalsAntimicrobial |
| spellingShingle | C Ogbiko CJ Eboka AD Tambuwal Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening Journal of Applied Sciences and Environmental Management Plantago rugelii Antioxidant Free Radicals Antimicrobial |
| title | Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening |
| title_full | Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening |
| title_fullStr | Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening |
| title_full_unstemmed | Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening |
| title_short | Extraction and Fractionation of Whole Black Seed Plantain (Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening |
| title_sort | extraction and fractionation of whole black seed plantain plantago rugelii decne for in vitro antioxidant antibacterial and phytochemical screening |
| topic | Plantago rugelii Antioxidant Free Radicals Antimicrobial |
| url | https://www.ajol.info/index.php/jasem/article/view/172634 |
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