A first attempt into the production of acylglycerol mixtures from Echium oil

Enzymatic glycerolysis of Echium oil (Echium plantagineum) has been carried out in the presence of four commercial lipases. Different pretreatment of the reaction mixture, such as high pressure homogenization and addition of food grade monoolein as an emulsifier, were evaluated to test their influen...

Full description

Bibliographic Details
Main Authors: Luis eVázquez, Alejandro eJordán, Guillermo eReglero, Carlos F Torres
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-01-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fbioe.2015.00208/full
_version_ 1818412866288484352
author Luis eVázquez
Alejandro eJordán
Guillermo eReglero
Guillermo eReglero
Carlos F Torres
author_facet Luis eVázquez
Alejandro eJordán
Guillermo eReglero
Guillermo eReglero
Carlos F Torres
author_sort Luis eVázquez
collection DOAJ
description Enzymatic glycerolysis of Echium oil (Echium plantagineum) has been carried out in the presence of four commercial lipases. Different pretreatment of the reaction mixture, such as high pressure homogenization and addition of food grade monoolein as an emulsifier, were evaluated to test their influence on the glycerolysis reaction. In addition, the impact of reducing temperature and the utilization of a solvent generally recognized as safe as a flavoring agent, such as limonene, were also investigated. Conversion of ca. 60-70 % of triacylglycerols, and production of ca. 25-30 % of monoacylglycerols were attained. Finally, at the best reaction conditions, the glycerolysis reaction was scaled-up at pilot plant and the product mixture obtained, fractionated via molecular distillation. From this stage, two products were attained: a distillate containing 80 % of monoacylglycerols and a residue containing approximately 50% of diacylglycerols and 50 % of triacylglycerols. All these mixtures can be utilized as self-emulsifying vehicles for the formulation of bioactive substances and also as precursors for the production of structured bioactive lipids.
first_indexed 2024-12-14T10:54:07Z
format Article
id doaj.art-d0cf42b602b54d73ae7150b01dd7b77c
institution Directory Open Access Journal
issn 2296-4185
language English
last_indexed 2024-12-14T10:54:07Z
publishDate 2016-01-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Bioengineering and Biotechnology
spelling doaj.art-d0cf42b602b54d73ae7150b01dd7b77c2022-12-21T23:05:03ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852016-01-01310.3389/fbioe.2015.00208135627A first attempt into the production of acylglycerol mixtures from Echium oilLuis eVázquez0Alejandro eJordán1Guillermo eReglero2Guillermo eReglero3Carlos F Torres4CIALCIALCIALIMDEA-Food InstituteCIALEnzymatic glycerolysis of Echium oil (Echium plantagineum) has been carried out in the presence of four commercial lipases. Different pretreatment of the reaction mixture, such as high pressure homogenization and addition of food grade monoolein as an emulsifier, were evaluated to test their influence on the glycerolysis reaction. In addition, the impact of reducing temperature and the utilization of a solvent generally recognized as safe as a flavoring agent, such as limonene, were also investigated. Conversion of ca. 60-70 % of triacylglycerols, and production of ca. 25-30 % of monoacylglycerols were attained. Finally, at the best reaction conditions, the glycerolysis reaction was scaled-up at pilot plant and the product mixture obtained, fractionated via molecular distillation. From this stage, two products were attained: a distillate containing 80 % of monoacylglycerols and a residue containing approximately 50% of diacylglycerols and 50 % of triacylglycerols. All these mixtures can be utilized as self-emulsifying vehicles for the formulation of bioactive substances and also as precursors for the production of structured bioactive lipids.http://journal.frontiersin.org/Journal/10.3389/fbioe.2015.00208/fullLipasemonoacylglycerolGlycerolysisSelf-emulsifyingEchium oil
spellingShingle Luis eVázquez
Alejandro eJordán
Guillermo eReglero
Guillermo eReglero
Carlos F Torres
A first attempt into the production of acylglycerol mixtures from Echium oil
Frontiers in Bioengineering and Biotechnology
Lipase
monoacylglycerol
Glycerolysis
Self-emulsifying
Echium oil
title A first attempt into the production of acylglycerol mixtures from Echium oil
title_full A first attempt into the production of acylglycerol mixtures from Echium oil
title_fullStr A first attempt into the production of acylglycerol mixtures from Echium oil
title_full_unstemmed A first attempt into the production of acylglycerol mixtures from Echium oil
title_short A first attempt into the production of acylglycerol mixtures from Echium oil
title_sort first attempt into the production of acylglycerol mixtures from echium oil
topic Lipase
monoacylglycerol
Glycerolysis
Self-emulsifying
Echium oil
url http://journal.frontiersin.org/Journal/10.3389/fbioe.2015.00208/full
work_keys_str_mv AT luisevazquez afirstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT alejandroejordan afirstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT guillermoereglero afirstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT guillermoereglero afirstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT carlosftorres afirstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT luisevazquez firstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT alejandroejordan firstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT guillermoereglero firstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT guillermoereglero firstattemptintotheproductionofacylglycerolmixturesfromechiumoil
AT carlosftorres firstattemptintotheproductionofacylglycerolmixturesfromechiumoil