Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR

Efficient genetic editing of human intestinal organoids using ribonucleoprotein-based CRISPR

Bibliographic Details
Main Authors:	Nefeli Skoufou-Papoutsaki, Sam Adler, Paula D'Santos, Liz Mannion, Shenay Mehmed, Richard Kemp, Amy Smith, Francesca Perrone, Komal Nayak, Alasdair Russell, Matthias Zilbauer, Douglas J. Winton
Format:	Article
Language:	English
Published:	The Company of Biologists 2023-10-01
Series:	Disease Models & Mechanisms
Subjects:	pten genome editing organoids
Online Access:	http://dmm.biologists.org/content/16/10/dmm050279

Similar Items

Stem cells for organoids
by: Shutong Qian, et al.
Published: (2022-12-01)

Integrated multi-omics analyses on patient-derived CRC organoids highlight altered molecular pathways in colorectal cancer progression involving PTEN
by: Marta Codrich, et al.
Published: (2021-06-01)

TP53-PTEN-NF1 depletion in human brain organoids produces a glioma phenotype in vitro
by: Sanjay K. Singh, et al.
Published: (2023-10-01)

Modeling epigenetic modifications in renal development and disease with organoids and genome editing
by: Carmen Hurtado del Pozo, et al.
Published: (2018-11-01)

Chromatin Accessibility and Transcriptional Differences in Human Stem Cell-Derived Early-Stage Retinal Organoids
by: Melissa K. Jones, et al.
Published: (2022-10-01)

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9
by: Anne Huber, et al.
Published: (2023-03-01)

Obtaining purified human intestinal epithelia for single-cell analysis and organoid culture
by: Alexander D.B. Ross, et al.
Published: (2021-06-01)

Organoid research on human early development and beyond
by: Wen Lu, et al.
Published: (2022-10-01)

Modeling human neurodevelopmental diseases with brain organoids
by: Xiaoxiang Lu, et al.
Published: (2022-01-01)

Human Organoids for Predictive Toxicology Research and Drug Development
by: Toshikatsu Matsui, et al.
Published: (2021-11-01)

Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids
by: Adriana Martinez-Silgado, et al.
Published: (2022-09-01)

Genome engineering of stem cell organoids for disease modeling
by: Yingmin Sun, et al.
Published: (2017-01-01)

Patient-Derived Organoids in Precision Medicine: Drug Screening, Organoid-on-a-Chip and Living Organoid Biobank
by: Zilong Zhou, et al.
Published: (2021-12-01)

Organoid and organoid extracellular vesicles for osteoporotic fractures therapy: Current status and future perspectives
by: Han Liu, et al.
Published: (2023-07-01)

Organoids to model the endometrium: implantation and beyond
by: Thomas M Rawlings, et al.
Published: (2021-09-01)

Human Brain Organoids and Consciousness: Moral Claims and Epistemic Uncertainty
by: Eliza Goddard, et al.
Published: (2023-02-01)

A functional genetic toolbox for human tissue-derived organoids
by: Dawei Sun, et al.
Published: (2021-10-01)

Microfluidics and Organoids, the Power Couple of Developmental Biology and Oncology Studies
by: Laura Ann Hetzel, et al.
Published: (2023-06-01)

Retinal Organoids from an AIPL1 CRISPR/Cas9 Knockout Cell Line Successfully Recapitulate the Molecular Features of LCA4 Disease
by: Pedro R. L. Perdigão, et al.
Published: (2023-03-01)

Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
by: Dedrick Kok Hong Chan, et al.
Published: (2023-03-01)

Cerebral Organoids as an Experimental Platform for Human Neurogenomics
by: Tomasz J. Nowakowski, et al.
Published: (2022-09-01)

Organoids and Colorectal Cancer
by: Antonio Barbáchano, et al.
Published: (2021-05-01)

CRISPR Ribonucleoprotein-Mediated Precise Editing of Multiple Genes in Porcine Fibroblasts
by: Xiaochen Guo, et al.
Published: (2024-02-01)

Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System
by: Yujin Ahn, et al.
Published: (2021-08-01)

Human Brain Organoids in Migraine Research: Pathogenesis and Drug Development
by: Parisa Gazerani
Published: (2023-02-01)

Establishing transgenic murine esophageal organoids
by: Kyung-Pil Ko, et al.
Published: (2022-06-01)

Organoids: opportunities and challenges of cancer therapy
by: Xianjie Jiang, et al.
Published: (2023-07-01)

Single-cell transcriptomics of lung organoids
by: Ye Na Song, et al.
Published: (2021-10-01)

Editorial: Human organoid technology for virus research
by: Dasja Pajkrt, et al.
Published: (2023-03-01)

The role of organoids in cancer research
by: Zhen Fang, et al.
Published: (2023-08-01)

Liver organoid culture methods
by: Yiqing Hu, et al.
Published: (2023-11-01)

Integration of Sensors in Gastrointestinal Organoid Culture for Biological Analysis
by: Ge-Ah Kim, et al.
Published: (2018-01-01)

Brain Organoids—A Bottom-Up Approach for Studying Human Neurodevelopment
by: Eyal Karzbrun, et al.
Published: (2019-01-01)

Disease modeling in organoid cultures: a new tool for studying viruses
by: Jaewon Cho, et al.
Published: (2022-07-01)

Assessing kidney development and disease using kidney organoids and CRISPR engineering
by: Wajima Safi, et al.
Published: (2022-09-01)

Organoids: Construction and Application in Gastric Cancer
by: Chengdong Huo, et al.
Published: (2023-05-01)

Methodologies for Generating Brain Organoids to Model Viral Pathogenesis in the CNS
by: Hannah K. Hopkins, et al.
Published: (2021-11-01)

Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
by: Qizhi Liu, et al.
Published: (2018-08-01)

Revolutionizing Disease Modeling: The Emergence of Organoids in Cellular Systems
by: Rita Silva-Pedrosa, et al.
Published: (2023-03-01)

Recent advances and challenges in organoid-on-a-chip technology
by: Intan Rosalina Suhito, et al.
Published: (2022-04-01)