Effects of grape seed procyanidins on antioxidant function, barrier function, microbial community, and metabolites of cecum in geese
ABSTRACT: The gut is the first line of defense for body health and is essential to the overall health of geese. Grape seed procyanidins (GSPs) are proverbial for their antioxidant, anti-inflammatory, and microflora-regulating capabilities. This study aimed to inquire into the influences of dietary G...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123003978 |
| _version_ | 1827862307376463872 |
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| author | Chao Deng Yan Zhai Xu Yang Zhexiu Chen Qinghong Li Ruirong Hao |
| author_facet | Chao Deng Yan Zhai Xu Yang Zhexiu Chen Qinghong Li Ruirong Hao |
| author_sort | Chao Deng |
| collection | DOAJ |
| description | ABSTRACT: The gut is the first line of defense for body health and is essential to the overall health of geese. Grape seed procyanidins (GSPs) are proverbial for their antioxidant, anti-inflammatory, and microflora-regulating capabilities. This study aimed to inquire into the influences of dietary GSPs on the intestinal antioxidant function, barrier function, microflora, and metabolites of geese based on 16S rRNA sequencing and metabolomics. In total, 240 twenty-one-day-old Sichuan white geese were randomly divided into 4 groups, each of which was supplied with 1 of 4 diets: basal diet or a basal diet supplemented with 50, 100, or 150 mg/kg GSPs. Diets supplemented with GSPs at different concentrations significantly increased the total antioxidant capacity and superoxide dismutase activity in cecal mucosa (P < 0.001). Dietary supplementation with 50 or 100 mg/kg GSPs significantly increased catalase activity (P < 0.001). The serum diamine oxidase, D-lactic acid, and endotoxin concentrations were decreased by GSP supplementation in the goose diet. Dietary GSP supplementation increased microbial richness and diversity, enhanced the relative abundance of Firmicutes, and decreased that of Bacteroidetes in the cecum. Diets supplemented with 50 or 100 mg/kg GSPs enriched Eubacterium coprostanoligenes and Faecalibacterium. Dietary GSPs substantially raised the acetic and propionic acid concentrations in the cecum. The butyric acid concentration increased when the GSP dosage was 50 or 100 mg/kg. Additionally, dietary GSPs increased the levels of metabolites that belong to lipids and lipid-like molecules or organic acids and derivatives. Dietary GSP supplementation at 100 or 150 mg/kg reduced the levels of spermine (a source of cytotoxic metabolites) and N-acetylputrescine, which promotes in-vivo inflammation. In conclusion, dietary supplementation with GSPs was beneficial to gut health in geese. Dietary GSPs improved antioxidant activity; protected intestinal barrier integrity; increased the abundance and diversity of cecal microflora; promoted the proliferation of some beneficial bacteria; increased the production of acetic, propionic, and butyric acids in the cecum; and downregulated metabolites associated with cytotoxicity and inflammation. These results offer a strategy for promoting intestinal health in farmed geese. |
| first_indexed | 2024-03-12T13:51:22Z |
| format | Article |
| id | doaj.art-d0d61e63f1824ae3b6f0ebd1cd91aa70 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-12T13:51:22Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-d0d61e63f1824ae3b6f0ebd1cd91aa702023-08-23T04:32:39ZengElsevierPoultry Science0032-57912023-09-011029102878Effects of grape seed procyanidins on antioxidant function, barrier function, microbial community, and metabolites of cecum in geeseChao Deng0Yan Zhai1Xu Yang2Zhexiu Chen3Qinghong Li4Ruirong Hao5College of Animal Science, Shanxi Agricultural University, Taigu 030800, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030800, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030800, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030800, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030800, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030800, China; Key Laboratory of Farm Animal Genetic Resources Exploration and Breeding of Shanxi Province, Taigu 030800, China; Corresponding author:ABSTRACT: The gut is the first line of defense for body health and is essential to the overall health of geese. Grape seed procyanidins (GSPs) are proverbial for their antioxidant, anti-inflammatory, and microflora-regulating capabilities. This study aimed to inquire into the influences of dietary GSPs on the intestinal antioxidant function, barrier function, microflora, and metabolites of geese based on 16S rRNA sequencing and metabolomics. In total, 240 twenty-one-day-old Sichuan white geese were randomly divided into 4 groups, each of which was supplied with 1 of 4 diets: basal diet or a basal diet supplemented with 50, 100, or 150 mg/kg GSPs. Diets supplemented with GSPs at different concentrations significantly increased the total antioxidant capacity and superoxide dismutase activity in cecal mucosa (P < 0.001). Dietary supplementation with 50 or 100 mg/kg GSPs significantly increased catalase activity (P < 0.001). The serum diamine oxidase, D-lactic acid, and endotoxin concentrations were decreased by GSP supplementation in the goose diet. Dietary GSP supplementation increased microbial richness and diversity, enhanced the relative abundance of Firmicutes, and decreased that of Bacteroidetes in the cecum. Diets supplemented with 50 or 100 mg/kg GSPs enriched Eubacterium coprostanoligenes and Faecalibacterium. Dietary GSPs substantially raised the acetic and propionic acid concentrations in the cecum. The butyric acid concentration increased when the GSP dosage was 50 or 100 mg/kg. Additionally, dietary GSPs increased the levels of metabolites that belong to lipids and lipid-like molecules or organic acids and derivatives. Dietary GSP supplementation at 100 or 150 mg/kg reduced the levels of spermine (a source of cytotoxic metabolites) and N-acetylputrescine, which promotes in-vivo inflammation. In conclusion, dietary supplementation with GSPs was beneficial to gut health in geese. Dietary GSPs improved antioxidant activity; protected intestinal barrier integrity; increased the abundance and diversity of cecal microflora; promoted the proliferation of some beneficial bacteria; increased the production of acetic, propionic, and butyric acids in the cecum; and downregulated metabolites associated with cytotoxicity and inflammation. These results offer a strategy for promoting intestinal health in farmed geese.http://www.sciencedirect.com/science/article/pii/S0032579123003978grape seed procyanidingooseintestinal antioxidant functioncecal microbial communitymetabolite |
| spellingShingle | Chao Deng Yan Zhai Xu Yang Zhexiu Chen Qinghong Li Ruirong Hao Effects of grape seed procyanidins on antioxidant function, barrier function, microbial community, and metabolites of cecum in geese Poultry Science grape seed procyanidin goose intestinal antioxidant function cecal microbial community metabolite |
| title | Effects of grape seed procyanidins on antioxidant function, barrier function, microbial community, and metabolites of cecum in geese |
| title_full | Effects of grape seed procyanidins on antioxidant function, barrier function, microbial community, and metabolites of cecum in geese |
| title_fullStr | Effects of grape seed procyanidins on antioxidant function, barrier function, microbial community, and metabolites of cecum in geese |
| title_full_unstemmed | Effects of grape seed procyanidins on antioxidant function, barrier function, microbial community, and metabolites of cecum in geese |
| title_short | Effects of grape seed procyanidins on antioxidant function, barrier function, microbial community, and metabolites of cecum in geese |
| title_sort | effects of grape seed procyanidins on antioxidant function barrier function microbial community and metabolites of cecum in geese |
| topic | grape seed procyanidin goose intestinal antioxidant function cecal microbial community metabolite |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123003978 |
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