Some responses of dry farming wheat to osmotic stresses in hydroponics culture
Osmotic stress is one of the major factors that significantly reduce yields in dry areas. Plants respond to this abiotic stress at physiological and molecular levels. Many genes are induced under stress conditions by transcription factors. Dehydration responsive element binding (DREB) protein is a s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Ferdowsi University of Mashhad
2009-07-01
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| Series: | Journal of Cell and Molecular Research |
| Subjects: | |
| Online Access: | https://jcmr.um.ac.ir/article_25775_85685566292b8ae5947b4c862d61ab64.pdf |
| _version_ | 1829463745995210752 |
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| author | Sasan Mohsenzadeh Sahar Sadeghi Hassan Mohabatkar Ali Niazi |
| author_facet | Sasan Mohsenzadeh Sahar Sadeghi Hassan Mohabatkar Ali Niazi |
| author_sort | Sasan Mohsenzadeh |
| collection | DOAJ |
| description | Osmotic stress is one of the major factors that significantly reduce yields in dry areas. Plants respond to this
abiotic stress at physiological and molecular levels. Many genes are induced under stress conditions by
transcription factors. Dehydration responsive element binding (DREB) protein is a subfamily of AP2/ERF
transcription factors which control expression of many osmotic stress-inducible genes. In this study, 21 days
old seedlings of Sardari cultivar, dry farming bread wheat transferred into hydroponics culture using Hoagland
solution. Osmotic stress treatments performed with adding 100, 200 and 400 g/l poly-ethylene glycol 6000 to
hydroponics culture to obtain –0.15, –0.49, and –1.76 MPa water potential, respectively. After the seedlings
were withered and colorless, relative water content, dry weight, and photosynthesis measured. In addition, RTPCR,
and cDNA sequencing carried out. Molecular analysis of DREB translated protein sequence performed
by DNAMAN, BLASTN, Pfam and PROSITE software. Results showed that osmotic stress decreased relative
water content, root and shoot dry weight and net photosynthesis rate in comparison to control, significantly (P
< 0.05). Sequence alignment indicated 98% homology with other Triticum aestivum DREB protein mRNA.
There was an AP2 domain in the translated protein with three -sheets and one -helix and contains the Val14
and Glu19 amino acids. An EST Sequence deposited in NCBI GenBank database with the accession number of
ES466900. |
| first_indexed | 2024-12-13T13:36:12Z |
| format | Article |
| id | doaj.art-d0db75e8d9f84a7c9f92449a34a87fea |
| institution | Directory Open Access Journal |
| issn | 2008-9147 2717-3364 |
| language | English |
| last_indexed | 2024-12-13T13:36:12Z |
| publishDate | 2009-07-01 |
| publisher | Ferdowsi University of Mashhad |
| record_format | Article |
| series | Journal of Cell and Molecular Research |
| spelling | doaj.art-d0db75e8d9f84a7c9f92449a34a87fea2022-12-21T23:43:47ZengFerdowsi University of MashhadJournal of Cell and Molecular Research2008-91472717-33642009-07-0112838910.22067/jcmr.v1i2.322425775Some responses of dry farming wheat to osmotic stresses in hydroponics cultureSasan MohsenzadehSahar Sadeghi0Hassan Mohabatkar1Ali Niazi2Shiraz UniversityShiraz UniversityShiraz UniversityOsmotic stress is one of the major factors that significantly reduce yields in dry areas. Plants respond to this abiotic stress at physiological and molecular levels. Many genes are induced under stress conditions by transcription factors. Dehydration responsive element binding (DREB) protein is a subfamily of AP2/ERF transcription factors which control expression of many osmotic stress-inducible genes. In this study, 21 days old seedlings of Sardari cultivar, dry farming bread wheat transferred into hydroponics culture using Hoagland solution. Osmotic stress treatments performed with adding 100, 200 and 400 g/l poly-ethylene glycol 6000 to hydroponics culture to obtain –0.15, –0.49, and –1.76 MPa water potential, respectively. After the seedlings were withered and colorless, relative water content, dry weight, and photosynthesis measured. In addition, RTPCR, and cDNA sequencing carried out. Molecular analysis of DREB translated protein sequence performed by DNAMAN, BLASTN, Pfam and PROSITE software. Results showed that osmotic stress decreased relative water content, root and shoot dry weight and net photosynthesis rate in comparison to control, significantly (P < 0.05). Sequence alignment indicated 98% homology with other Triticum aestivum DREB protein mRNA. There was an AP2 domain in the translated protein with three -sheets and one -helix and contains the Val14 and Glu19 amino acids. An EST Sequence deposited in NCBI GenBank database with the accession number of ES466900.https://jcmr.um.ac.ir/article_25775_85685566292b8ae5947b4c862d61ab64.pdfap2 domaindrebosmotic stressphysiological responseswheat |
| spellingShingle | Sasan Mohsenzadeh Sahar Sadeghi Hassan Mohabatkar Ali Niazi Some responses of dry farming wheat to osmotic stresses in hydroponics culture Journal of Cell and Molecular Research ap2 domain dreb osmotic stress physiological responses wheat |
| title | Some responses of dry farming wheat to osmotic stresses in hydroponics culture |
| title_full | Some responses of dry farming wheat to osmotic stresses in hydroponics culture |
| title_fullStr | Some responses of dry farming wheat to osmotic stresses in hydroponics culture |
| title_full_unstemmed | Some responses of dry farming wheat to osmotic stresses in hydroponics culture |
| title_short | Some responses of dry farming wheat to osmotic stresses in hydroponics culture |
| title_sort | some responses of dry farming wheat to osmotic stresses in hydroponics culture |
| topic | ap2 domain dreb osmotic stress physiological responses wheat |
| url | https://jcmr.um.ac.ir/article_25775_85685566292b8ae5947b4c862d61ab64.pdf |
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