Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol
The objective of the study was to compare the effect of calcium peroxide and citric acid on the activity of acid phosphatase (ACP), alkaline phosphatase (ALP), and dehydrogenases (DHA) in uncontaminated soil and soil contaminated with petrol. The experiment was carried out on samples of loamy sand u...
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| Format: | Article |
| Language: | English |
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De Gruyter
2020-02-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2020-0002 |
| _version_ | 1818731828870119424 |
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| author | Curyło Kornel Telesiński Arkadiusz |
| author_facet | Curyło Kornel Telesiński Arkadiusz |
| author_sort | Curyło Kornel |
| collection | DOAJ |
| description | The objective of the study was to compare the effect of calcium peroxide and citric acid on the activity of acid phosphatase (ACP), alkaline phosphatase (ALP), and dehydrogenases (DHA) in uncontaminated soil and soil contaminated with petrol. The experiment was carried out on samples of loamy sand under laboratory conditions. Petrol was introduced to soil samples at a dose of 0 and 50 g·kg 1 DM, as well as calcium peroxide or citric acid at a dose of 0, 50, 100, or 150 mg·kg 1 DM. The humidity of the samples was brought to 60% maximum water holding capacity, and the samples were incubated at 20°C for 8 weeks. Enzyme activity was determined on days 1, 14, 28, and 56. The obtained results demonstrated that the addition of calcium peroxide and citric acid did not result in significant changes in the activity of the determined enzymes in uncontaminated soil. However, it was observed that the application of calcium peroxide, particularly at the dose of 150 mg·kg 1 DM, largely alleviated the impact of petrol on the enzymatic activity of the soil contaminated with petrol. Moreover, among the determined enzymes, the activity of DHA was found to be the best indicator of the effect of calcium peroxide on the soil ecosystem. |
| first_indexed | 2024-12-17T23:23:53Z |
| format | Article |
| id | doaj.art-d0e0d7c71e704c238a284eb899f31cf4 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-12-17T23:23:53Z |
| publishDate | 2020-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-d0e0d7c71e704c238a284eb899f31cf42022-12-21T21:28:49ZengDe GruyterOpen Life Sciences2391-54122020-02-01151122010.1515/biol-2020-0002biol-2020-0002Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrolCuryło Kornel0Telesiński Arkadiusz1Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology in Szczecin, 17 Słowackiego St., 71-434Szczecin, PolandDepartment of Plant Physiology and Biochemistry, West Pomeranian University of Technology in Szczecin, 17 Słowackiego St., 71-434Szczecin, PolandThe objective of the study was to compare the effect of calcium peroxide and citric acid on the activity of acid phosphatase (ACP), alkaline phosphatase (ALP), and dehydrogenases (DHA) in uncontaminated soil and soil contaminated with petrol. The experiment was carried out on samples of loamy sand under laboratory conditions. Petrol was introduced to soil samples at a dose of 0 and 50 g·kg 1 DM, as well as calcium peroxide or citric acid at a dose of 0, 50, 100, or 150 mg·kg 1 DM. The humidity of the samples was brought to 60% maximum water holding capacity, and the samples were incubated at 20°C for 8 weeks. Enzyme activity was determined on days 1, 14, 28, and 56. The obtained results demonstrated that the addition of calcium peroxide and citric acid did not result in significant changes in the activity of the determined enzymes in uncontaminated soil. However, it was observed that the application of calcium peroxide, particularly at the dose of 150 mg·kg 1 DM, largely alleviated the impact of petrol on the enzymatic activity of the soil contaminated with petrol. Moreover, among the determined enzymes, the activity of DHA was found to be the best indicator of the effect of calcium peroxide on the soil ecosystem.https://doi.org/10.1515/biol-2020-0002soil enzymatic activityloamy sandhydrocarbonsremediation |
| spellingShingle | Curyło Kornel Telesiński Arkadiusz Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol Open Life Sciences soil enzymatic activity loamy sand hydrocarbons remediation |
| title | Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol |
| title_full | Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol |
| title_fullStr | Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol |
| title_full_unstemmed | Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol |
| title_short | Use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol |
| title_sort | use of phosphatase and dehydrogenase activities in the assessment of calcium peroxide and citric acid effects in soil contaminated with petrol |
| topic | soil enzymatic activity loamy sand hydrocarbons remediation |
| url | https://doi.org/10.1515/biol-2020-0002 |
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