Effects of <i>Taraxacum officinale</i> on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids Content
Glioblastoma is an aggressive type of CNS tumor. The aim was to evaluate the antitumor activity of <i>Taraxacum officinale</i> (TO) extracts on U-138 MG glial cells and correlate it with the concentration of chicoric (ChA), chlorogenic (CGA), and caftaric (CA) acids (mg/mL) in the extrac...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Medical Sciences Forum |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-9992/21/1/18 |
| _version_ | 1827573947965636608 |
|---|---|
| author | Ala Fulga Ana Casian Igor Casian Svetlana Protopop Valentin Gudumac Olga Tagadiuc |
| author_facet | Ala Fulga Ana Casian Igor Casian Svetlana Protopop Valentin Gudumac Olga Tagadiuc |
| author_sort | Ala Fulga |
| collection | DOAJ |
| description | Glioblastoma is an aggressive type of CNS tumor. The aim was to evaluate the antitumor activity of <i>Taraxacum officinale</i> (TO) extracts on U-138 MG glial cells and correlate it with the concentration of chicoric (ChA), chlorogenic (CGA), and caftaric (CA) acids (mg/mL) in the extract. TO dry leaves were extracted with DMSO (dimethyl sulfoxide) and ethanol of different concentrations. The concentration of acids was determined by liquid chromatograph (Agilent 1260 with DAD). The viability of U-138 MG cells was assessed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) test (% of viable cells). The activity of TO was compared with that of doxorubicin. The best antitumor activity was shown by TO extracts prepared with DMSO (110,000 µg/L—17.3 ± 8%, which contained ChA—8976 × 10<sup>−6</sup> mg/mL, CGA—316.8 × 10<sup>−6</sup> mg/mL, CA—1628 × 10<sup>−6</sup> mg/mL), with 50% ethanol (150,000 µg/L—13.7 ± 3.2%, containing ChA—52,500 × 10<sup>−6</sup> mg/mL, CGA—1746 × 10<sup>−6</sup> mg/mL, CA—8460 × 10<sup>−6</sup> mg/mL) and with 80% ethanol (40,000 µg/L—16.1 ± 9%, containing ChA—904 × 10<sup>−6</sup> mg/mL, CGA—114.4 × 10<sup>−6</sup> mg/mL, CA—70.4 × 10<sup>−6</sup> mg/mL). TO extract activity was close to that of doxorubicin. In conclusion, the TO antitumor activity depends on the type of extractant and its concentration, as well as on the content of cinnamic acids. |
| first_indexed | 2024-03-08T20:29:23Z |
| format | Article |
| id | doaj.art-d0e29b22079f42c1a8db2e53ff3a99d9 |
| institution | Directory Open Access Journal |
| issn | 2673-9992 |
| language | English |
| last_indexed | 2024-03-08T20:29:23Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medical Sciences Forum |
| spelling | doaj.art-d0e29b22079f42c1a8db2e53ff3a99d92023-12-22T14:28:41ZengMDPI AGMedical Sciences Forum2673-99922023-04-012111810.3390/ECB2023-14363Effects of <i>Taraxacum officinale</i> on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids ContentAla Fulga0Ana Casian1Igor Casian2Svetlana Protopop3Valentin Gudumac4Olga Tagadiuc5Department of Biochemistry and Clinical Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, MD-2025 Chisinau, MoldovaDrug Research Center, Nicolae Testemitanu State University of Medicine and Pharmacy, MD-2004 Chisinau, MoldovaDrug Research Center, Nicolae Testemitanu State University of Medicine and Pharmacy, MD-2004 Chisinau, MoldovaDepartment of Biochemistry and Clinical Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, MD-2025 Chisinau, MoldovaLaboratory of Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, MD-2004 Chisinau, MoldovaDepartment of Biochemistry and Clinical Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, MD-2025 Chisinau, MoldovaGlioblastoma is an aggressive type of CNS tumor. The aim was to evaluate the antitumor activity of <i>Taraxacum officinale</i> (TO) extracts on U-138 MG glial cells and correlate it with the concentration of chicoric (ChA), chlorogenic (CGA), and caftaric (CA) acids (mg/mL) in the extract. TO dry leaves were extracted with DMSO (dimethyl sulfoxide) and ethanol of different concentrations. The concentration of acids was determined by liquid chromatograph (Agilent 1260 with DAD). The viability of U-138 MG cells was assessed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) test (% of viable cells). The activity of TO was compared with that of doxorubicin. The best antitumor activity was shown by TO extracts prepared with DMSO (110,000 µg/L—17.3 ± 8%, which contained ChA—8976 × 10<sup>−6</sup> mg/mL, CGA—316.8 × 10<sup>−6</sup> mg/mL, CA—1628 × 10<sup>−6</sup> mg/mL), with 50% ethanol (150,000 µg/L—13.7 ± 3.2%, containing ChA—52,500 × 10<sup>−6</sup> mg/mL, CGA—1746 × 10<sup>−6</sup> mg/mL, CA—8460 × 10<sup>−6</sup> mg/mL) and with 80% ethanol (40,000 µg/L—16.1 ± 9%, containing ChA—904 × 10<sup>−6</sup> mg/mL, CGA—114.4 × 10<sup>−6</sup> mg/mL, CA—70.4 × 10<sup>−6</sup> mg/mL). TO extract activity was close to that of doxorubicin. In conclusion, the TO antitumor activity depends on the type of extractant and its concentration, as well as on the content of cinnamic acids.https://www.mdpi.com/2673-9992/21/1/18<i>Taraxacum officinale</i>cinnamic acid derivativesglioblastomaU-138 MGMTT test |
| spellingShingle | Ala Fulga Ana Casian Igor Casian Svetlana Protopop Valentin Gudumac Olga Tagadiuc Effects of <i>Taraxacum officinale</i> on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids Content Medical Sciences Forum <i>Taraxacum officinale</i> cinnamic acid derivatives glioblastoma U-138 MG MTT test |
| title | Effects of <i>Taraxacum officinale</i> on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids Content |
| title_full | Effects of <i>Taraxacum officinale</i> on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids Content |
| title_fullStr | Effects of <i>Taraxacum officinale</i> on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids Content |
| title_full_unstemmed | Effects of <i>Taraxacum officinale</i> on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids Content |
| title_short | Effects of <i>Taraxacum officinale</i> on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids Content |
| title_sort | effects of i taraxacum officinale i on glioblastoma cell culture and their correlation with hydroxycinnamic acids content |
| topic | <i>Taraxacum officinale</i> cinnamic acid derivatives glioblastoma U-138 MG MTT test |
| url | https://www.mdpi.com/2673-9992/21/1/18 |
| work_keys_str_mv | AT alafulga effectsofitaraxacumofficinaleionglioblastomacellcultureandtheircorrelationwithhydroxycinnamicacidscontent AT anacasian effectsofitaraxacumofficinaleionglioblastomacellcultureandtheircorrelationwithhydroxycinnamicacidscontent AT igorcasian effectsofitaraxacumofficinaleionglioblastomacellcultureandtheircorrelationwithhydroxycinnamicacidscontent AT svetlanaprotopop effectsofitaraxacumofficinaleionglioblastomacellcultureandtheircorrelationwithhydroxycinnamicacidscontent AT valentingudumac effectsofitaraxacumofficinaleionglioblastomacellcultureandtheircorrelationwithhydroxycinnamicacidscontent AT olgatagadiuc effectsofitaraxacumofficinaleionglioblastomacellcultureandtheircorrelationwithhydroxycinnamicacidscontent |