Cyclooxygenase enzyme and PGE2 expression in patients with functional and non-functional pituitary adenomas
Abstract Background Pituitary adenomas as multifactorial intracranial neoplasms impose a massive burden of morbidity on patients and characterizing the molecular mechanism underlying their pathogenesis has received considerable attention. Despite the appealing role of cyclooxygenase enzymes and thei...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | BMC Endocrine Disorders |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12902-020-0515-8 |
| _version_ | 1818271536019144704 |
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| author | Nasrin Akbari Mohammad Ghorbani Vahid Salimi Alimohammad Alimohammadi Mohammad E. Khamseh Hamideh Akbari Mitra Nourbakhsh Alireza Sheikhi S. Fahimeh Taghavi Masoumeh Tavakoli-Yaraki |
| author_facet | Nasrin Akbari Mohammad Ghorbani Vahid Salimi Alimohammad Alimohammadi Mohammad E. Khamseh Hamideh Akbari Mitra Nourbakhsh Alireza Sheikhi S. Fahimeh Taghavi Masoumeh Tavakoli-Yaraki |
| author_sort | Nasrin Akbari |
| collection | DOAJ |
| description | Abstract Background Pituitary adenomas as multifactorial intracranial neoplasms impose a massive burden of morbidity on patients and characterizing the molecular mechanism underlying their pathogenesis has received considerable attention. Despite the appealing role of cyclooxygenase enzymes and their bioactive lipid products in cancer pathogenesis, their relevance to pituitary adenoma pathogenesis is debated and yet to be determined. Thus, the current study perused this relevance. Methods The expression level of the isoforms of cyclooxygenase (COX-1 and COX-2) was evaluated in hormone-secreting and in-active pituitary adenoma tumors and normal pituitary tissues through Real-Time PCR. The level of PGE2, as the main product of enzymes, was assessed using enzyme immunoassay kits in patients and healthy subjects. Results The results of the current study demonstrated that COX-1 and COX-2 expression levels were increased in pituitary tumors including non-functional pituitary adenoma (NFPA), acromegaly, Cushing’s disease and prolactinoma compared with normal pituitary tissues. A significant expression level of COX-2 was observed in NFPA compared with the other pituitary tumors. Furthermore, the COX-2 expression level was significantly increased in macroadenoma and invasive tumors. The level of PGE2 was consistent with COX enzymes enhanced in pituitary adenoma tumors compared with healthy pituitary tissue. A significant elevation in the PGE2 level was detected in NFPA compared with hormone-secreting pituitary tumors. Additionally, the PGE2 level was increased in macroadenoma compared with microadenoma and in invasive compared with non-invasive pituitary tumors. The diagnostic values of cyclooxygenase isoforms and PGE2 were considerable between patients and healthy groups; however, COX-2 revealed more value in distinguishing endocrinologically active and non-active pituitary tumors. Conclusions Data from the current study provides expression patterns of COX-1, COX-2 and PGE2 in prevalent pituitary tumors and their association with patients’ clinical features which may open up new molecular targets for early diagnosis/follow up of pituitary tumor growth. |
| first_indexed | 2024-12-12T21:27:44Z |
| format | Article |
| id | doaj.art-d0e397c57b33428d91b80e17043b5c95 |
| institution | Directory Open Access Journal |
| issn | 1472-6823 |
| language | English |
| last_indexed | 2024-12-12T21:27:44Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Endocrine Disorders |
| spelling | doaj.art-d0e397c57b33428d91b80e17043b5c952022-12-22T00:11:25ZengBMCBMC Endocrine Disorders1472-68232020-03-0120111210.1186/s12902-020-0515-8Cyclooxygenase enzyme and PGE2 expression in patients with functional and non-functional pituitary adenomasNasrin Akbari0Mohammad Ghorbani1Vahid Salimi2Alimohammad Alimohammadi3Mohammad E. Khamseh4Hamideh Akbari5Mitra Nourbakhsh6Alireza Sheikhi7S. Fahimeh Taghavi8Masoumeh Tavakoli-Yaraki9Department of Biochemistry, School of Medicine, Iran University of Medical SciencesDivision of Vascular and Endovascular Neurosurgery, Firoozgar Hospital, Iran University of Medical SciencesDepartment of Virology, School of Public Health, Tehran University of Medical SciencesIranian Legal Medicine OrganizationsEndocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS)Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS)Department of Biochemistry, School of Medicine, Iran University of Medical SciencesDepartment of Biochemistry, School of Medicine, Iran University of Medical SciencesDepartment of Biochemistry, School of Medicine, Iran University of Medical SciencesDepartment of Biochemistry, School of Medicine, Iran University of Medical SciencesAbstract Background Pituitary adenomas as multifactorial intracranial neoplasms impose a massive burden of morbidity on patients and characterizing the molecular mechanism underlying their pathogenesis has received considerable attention. Despite the appealing role of cyclooxygenase enzymes and their bioactive lipid products in cancer pathogenesis, their relevance to pituitary adenoma pathogenesis is debated and yet to be determined. Thus, the current study perused this relevance. Methods The expression level of the isoforms of cyclooxygenase (COX-1 and COX-2) was evaluated in hormone-secreting and in-active pituitary adenoma tumors and normal pituitary tissues through Real-Time PCR. The level of PGE2, as the main product of enzymes, was assessed using enzyme immunoassay kits in patients and healthy subjects. Results The results of the current study demonstrated that COX-1 and COX-2 expression levels were increased in pituitary tumors including non-functional pituitary adenoma (NFPA), acromegaly, Cushing’s disease and prolactinoma compared with normal pituitary tissues. A significant expression level of COX-2 was observed in NFPA compared with the other pituitary tumors. Furthermore, the COX-2 expression level was significantly increased in macroadenoma and invasive tumors. The level of PGE2 was consistent with COX enzymes enhanced in pituitary adenoma tumors compared with healthy pituitary tissue. A significant elevation in the PGE2 level was detected in NFPA compared with hormone-secreting pituitary tumors. Additionally, the PGE2 level was increased in macroadenoma compared with microadenoma and in invasive compared with non-invasive pituitary tumors. The diagnostic values of cyclooxygenase isoforms and PGE2 were considerable between patients and healthy groups; however, COX-2 revealed more value in distinguishing endocrinologically active and non-active pituitary tumors. Conclusions Data from the current study provides expression patterns of COX-1, COX-2 and PGE2 in prevalent pituitary tumors and their association with patients’ clinical features which may open up new molecular targets for early diagnosis/follow up of pituitary tumor growth.http://link.springer.com/article/10.1186/s12902-020-0515-8Pituitary adenomaCyclooxygenase-1Cyclooxygenase-2PGE2 |
| spellingShingle | Nasrin Akbari Mohammad Ghorbani Vahid Salimi Alimohammad Alimohammadi Mohammad E. Khamseh Hamideh Akbari Mitra Nourbakhsh Alireza Sheikhi S. Fahimeh Taghavi Masoumeh Tavakoli-Yaraki Cyclooxygenase enzyme and PGE2 expression in patients with functional and non-functional pituitary adenomas BMC Endocrine Disorders Pituitary adenoma Cyclooxygenase-1 Cyclooxygenase-2 PGE2 |
| title | Cyclooxygenase enzyme and PGE2 expression in patients with functional and non-functional pituitary adenomas |
| title_full | Cyclooxygenase enzyme and PGE2 expression in patients with functional and non-functional pituitary adenomas |
| title_fullStr | Cyclooxygenase enzyme and PGE2 expression in patients with functional and non-functional pituitary adenomas |
| title_full_unstemmed | Cyclooxygenase enzyme and PGE2 expression in patients with functional and non-functional pituitary adenomas |
| title_short | Cyclooxygenase enzyme and PGE2 expression in patients with functional and non-functional pituitary adenomas |
| title_sort | cyclooxygenase enzyme and pge2 expression in patients with functional and non functional pituitary adenomas |
| topic | Pituitary adenoma Cyclooxygenase-1 Cyclooxygenase-2 PGE2 |
| url | http://link.springer.com/article/10.1186/s12902-020-0515-8 |
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