ISOLATION AND PURIFICATION OF LYSOZYME FROM THE HEN EGG WHITE
The aim of the research was the development of the method of lysozyme isolation from hen egg proteins. Lysozyme was isolated by differential heat denaturation of proteins with changing of the medium pH value, followed by neutralization, dialysis and additional purification by gel chromatography on S...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2015-12-01
|
Series: | Biotechnologia Acta |
Subjects: | |
Online Access: | http://biotechnology.kiev.ua/images/stories/6_2015/dekina_6_2015.pdf |
_version_ | 1797722231015473152 |
---|---|
author | S. S. I. I. A. M. M. G. V. A. |
author_facet | S. S. I. I. A. M. M. G. V. A. |
author_sort | S. S. |
collection | DOAJ |
description | The aim of the research was the development of the method of lysozyme isolation from hen egg proteins. Lysozyme was isolated by differential heat denaturation of proteins with changing of the medium pH value, followed by neutralization, dialysis and additional purification by gel chromatography on Sephadex G-50. Activity was determined by bacteriolytic method (with Micrococcus lysodeikticus 4698 as a substrate). The enzyme purity and molecular mass were determined using SDS-electrophoresis and massspectrometry.
The method of lysozyme isolation from hen egg proteins with the enzyme yield of 3.2 ± 0.2% and bacteriolytic activity of 22 025 ± 1 500 U/mg is modified. According to electrophoresis data, the isolated enzyme is characterized by high degree of purity (~95–98%) and is comparable with lysozyme of AppliChem company by main physical and chemical characteristics. The obtaining product is stored in a crystalline form at low temperature (–24 оC) for 9 months. The proposed method allows obtaining active and stable lysozyme with high purity from hen egg protein in laboratory conditions for the usage in biotechnology. |
first_indexed | 2024-03-12T09:45:28Z |
format | Article |
id | doaj.art-d0ed5907a3ad4a3c80d3ae6842de43fc |
institution | Directory Open Access Journal |
issn | 2410-7751 2410-776X |
language | English |
last_indexed | 2024-03-12T09:45:28Z |
publishDate | 2015-12-01 |
publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
record_format | Article |
series | Biotechnologia Acta |
spelling | doaj.art-d0ed5907a3ad4a3c80d3ae6842de43fc2023-09-02T12:58:56ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2015-12-0186414710.15407/biotech8.06.041ISOLATION AND PURIFICATION OF LYSOZYME FROM THE HEN EGG WHITES. S. 0 I. I. 1A. M. 2 M. G. 3V. A. 4DekinaRomanovska OvsepyanBodyul ToptikovThe aim of the research was the development of the method of lysozyme isolation from hen egg proteins. Lysozyme was isolated by differential heat denaturation of proteins with changing of the medium pH value, followed by neutralization, dialysis and additional purification by gel chromatography on Sephadex G-50. Activity was determined by bacteriolytic method (with Micrococcus lysodeikticus 4698 as a substrate). The enzyme purity and molecular mass were determined using SDS-electrophoresis and massspectrometry. The method of lysozyme isolation from hen egg proteins with the enzyme yield of 3.2 ± 0.2% and bacteriolytic activity of 22 025 ± 1 500 U/mg is modified. According to electrophoresis data, the isolated enzyme is characterized by high degree of purity (~95–98%) and is comparable with lysozyme of AppliChem company by main physical and chemical characteristics. The obtaining product is stored in a crystalline form at low temperature (–24 оC) for 9 months. The proposed method allows obtaining active and stable lysozyme with high purity from hen egg protein in laboratory conditions for the usage in biotechnology.http://biotechnology.kiev.ua/images/stories/6_2015/dekina_6_2015.pdfhen egg proteinlysozyme |
spellingShingle | S. S. I. I. A. M. M. G. V. A. ISOLATION AND PURIFICATION OF LYSOZYME FROM THE HEN EGG WHITE Biotechnologia Acta hen egg protein lysozyme |
title | ISOLATION AND PURIFICATION OF LYSOZYME FROM THE HEN EGG WHITE |
title_full | ISOLATION AND PURIFICATION OF LYSOZYME FROM THE HEN EGG WHITE |
title_fullStr | ISOLATION AND PURIFICATION OF LYSOZYME FROM THE HEN EGG WHITE |
title_full_unstemmed | ISOLATION AND PURIFICATION OF LYSOZYME FROM THE HEN EGG WHITE |
title_short | ISOLATION AND PURIFICATION OF LYSOZYME FROM THE HEN EGG WHITE |
title_sort | isolation and purification of lysozyme from the hen egg white |
topic | hen egg protein lysozyme |
url | http://biotechnology.kiev.ua/images/stories/6_2015/dekina_6_2015.pdf |
work_keys_str_mv | AT ss isolationandpurificationoflysozymefromtheheneggwhite AT ii isolationandpurificationoflysozymefromtheheneggwhite AT am isolationandpurificationoflysozymefromtheheneggwhite AT mg isolationandpurificationoflysozymefromtheheneggwhite AT va isolationandpurificationoflysozymefromtheheneggwhite |