Potential role of Albizia lebbeck and Emblica officinalis on smooth muscle contractions in experimental animal models
Background and objective: Spasms are involuntary muscular contractions commonly seen frequently. This study used isolated tissue preparations to test the efficacy of Albizia lebbeck (A.L) and Emblica officinalis (E.O) extracts for spasmolytic activity. Materials and methods: The herbal extracts were...
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| Format: | Article |
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Elsevier
2023-10-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S101836472300280X |
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| author | R. Bansilal Tiwari Mohammed Naseeruddin Inamdar Raha Orfali Syed Mohammed Basheeruddin Asdaq Mamdouh Allahyani Abdulelah Aljuaid Abdulaziz Alsharif Mehnaz Kamal Ahad Amer Alsaiari Mazen Almehmadi Mohd Imran Sultan Alshehri Moneer E. Almadani Syed Imam Rabbani |
| author_facet | R. Bansilal Tiwari Mohammed Naseeruddin Inamdar Raha Orfali Syed Mohammed Basheeruddin Asdaq Mamdouh Allahyani Abdulelah Aljuaid Abdulaziz Alsharif Mehnaz Kamal Ahad Amer Alsaiari Mazen Almehmadi Mohd Imran Sultan Alshehri Moneer E. Almadani Syed Imam Rabbani |
| author_sort | R. Bansilal Tiwari |
| collection | DOAJ |
| description | Background and objective: Spasms are involuntary muscular contractions commonly seen frequently. This study used isolated tissue preparations to test the efficacy of Albizia lebbeck (A.L) and Emblica officinalis (E.O) extracts for spasmolytic activity. Materials and methods: The herbal extracts were tested in isolated guinea pig ileum, rat uterus, rat fundus, and rabbit jejunum. Histamine was used as spasmogen in guinea pig ileum, while 5-hydroxytryptamine (5-HT) was in rat uterus and rat fundus. Spontaneous contractions' amplitude and frequency were recorded in the rabbit jejunum after administering herbal extracts. The influence of the extracts on smooth muscle contraction was calculated and statistically analyzed by one-way ANOVA. The P value was kept at <0.05 for all statistical analyses to consider it significant. Results: Observation from the present study indicated that A.L significantly (p < 0.05) enhanced the contraction induced by histamine and 5-HT in guinea pig ileum (50 mcg/ml) and rat fundus (150 mcg/ml), respectively. In the rabbit jejunum, the amplitude and frequency of contraction were significantly (p < 0.05) reduced at 500 mcg/ml. E.O. was found to suppress the spasmogenic (histamine and 5-HT) at doses beyond 150 mcg/ml and, in rabbit jejunum, enhanced the amplitude and frequency of contraction at 50 and 150 mcg/ml. The IC50 values for E.O. in guinea pig ileum, rat uterus, and rat fundus were 35.2, 50.3, and 124.7 mcg/ml, respectively. Conclusion: The observation suggests that A.L enhanced smooth muscle contraction in the presence of known spasmogens and reduced it in the absence. Opposite effects were found for E.O., where it reduced contraction in the presence of spasmogens and increased in the absence. These findings suggest potential spasmogenic/spasmolytic activities of the tested extracts. |
| first_indexed | 2024-03-12T01:21:53Z |
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| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-12T01:21:53Z |
| publishDate | 2023-10-01 |
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| series | Journal of King Saud University: Science |
| spelling | doaj.art-d0f59c76f3cc4361b5c1a7b7153938ab2023-09-13T04:24:43ZengElsevierJournal of King Saud University: Science1018-36472023-10-01357102818Potential role of Albizia lebbeck and Emblica officinalis on smooth muscle contractions in experimental animal modelsR. Bansilal Tiwari0Mohammed Naseeruddin Inamdar1Raha Orfali2Syed Mohammed Basheeruddin Asdaq3Mamdouh Allahyani4Abdulelah Aljuaid5Abdulaziz Alsharif6Mehnaz Kamal7Ahad Amer Alsaiari8Mazen Almehmadi9Mohd Imran10Sultan Alshehri11Moneer E. Almadani12Syed Imam Rabbani13Department of Pharmacology, Al-Ameen College of Pharmacy, Bangalore, IndiaDepartment of Pharmacology, East West College of Pharmacy, Bangalore, IndiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah, 13713 Riyadh, Saudi Arabia; Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah, 13713, Riyadh, Saudi Arabia; Corresponding author.Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Northern Border University, Rafha 91911, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, AlMaarefa University, Ad Diriyah 13713, Saudi ArabiaDepartment of Clinical Medicine, College of Medicine, AlMaarefa University, Dariyah, Riyadh 13713, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraydah 51452, Saudi ArabiaBackground and objective: Spasms are involuntary muscular contractions commonly seen frequently. This study used isolated tissue preparations to test the efficacy of Albizia lebbeck (A.L) and Emblica officinalis (E.O) extracts for spasmolytic activity. Materials and methods: The herbal extracts were tested in isolated guinea pig ileum, rat uterus, rat fundus, and rabbit jejunum. Histamine was used as spasmogen in guinea pig ileum, while 5-hydroxytryptamine (5-HT) was in rat uterus and rat fundus. Spontaneous contractions' amplitude and frequency were recorded in the rabbit jejunum after administering herbal extracts. The influence of the extracts on smooth muscle contraction was calculated and statistically analyzed by one-way ANOVA. The P value was kept at <0.05 for all statistical analyses to consider it significant. Results: Observation from the present study indicated that A.L significantly (p < 0.05) enhanced the contraction induced by histamine and 5-HT in guinea pig ileum (50 mcg/ml) and rat fundus (150 mcg/ml), respectively. In the rabbit jejunum, the amplitude and frequency of contraction were significantly (p < 0.05) reduced at 500 mcg/ml. E.O. was found to suppress the spasmogenic (histamine and 5-HT) at doses beyond 150 mcg/ml and, in rabbit jejunum, enhanced the amplitude and frequency of contraction at 50 and 150 mcg/ml. The IC50 values for E.O. in guinea pig ileum, rat uterus, and rat fundus were 35.2, 50.3, and 124.7 mcg/ml, respectively. Conclusion: The observation suggests that A.L enhanced smooth muscle contraction in the presence of known spasmogens and reduced it in the absence. Opposite effects were found for E.O., where it reduced contraction in the presence of spasmogens and increased in the absence. These findings suggest potential spasmogenic/spasmolytic activities of the tested extracts.http://www.sciencedirect.com/science/article/pii/S101836472300280XAlbizia lebbeckEmblica officinalisSpasmolyticsIsolated tissue preparations |
| spellingShingle | R. Bansilal Tiwari Mohammed Naseeruddin Inamdar Raha Orfali Syed Mohammed Basheeruddin Asdaq Mamdouh Allahyani Abdulelah Aljuaid Abdulaziz Alsharif Mehnaz Kamal Ahad Amer Alsaiari Mazen Almehmadi Mohd Imran Sultan Alshehri Moneer E. Almadani Syed Imam Rabbani Potential role of Albizia lebbeck and Emblica officinalis on smooth muscle contractions in experimental animal models Journal of King Saud University: Science Albizia lebbeck Emblica officinalis Spasmolytics Isolated tissue preparations |
| title | Potential role of Albizia lebbeck and Emblica officinalis on smooth muscle contractions in experimental animal models |
| title_full | Potential role of Albizia lebbeck and Emblica officinalis on smooth muscle contractions in experimental animal models |
| title_fullStr | Potential role of Albizia lebbeck and Emblica officinalis on smooth muscle contractions in experimental animal models |
| title_full_unstemmed | Potential role of Albizia lebbeck and Emblica officinalis on smooth muscle contractions in experimental animal models |
| title_short | Potential role of Albizia lebbeck and Emblica officinalis on smooth muscle contractions in experimental animal models |
| title_sort | potential role of albizia lebbeck and emblica officinalis on smooth muscle contractions in experimental animal models |
| topic | Albizia lebbeck Emblica officinalis Spasmolytics Isolated tissue preparations |
| url | http://www.sciencedirect.com/science/article/pii/S101836472300280X |
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