Application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase-positive staphylococci under the conditions related to artisanal cheese fermentation
A quantitative analysis of Staphylococcus aureus growth was carried out in milk fermented with the mesophilic mixed-strain culture of lactic acid bacteria (LAB), in relation to the conditions prevailing during artisanal ewes' lump cheese production. Both the temperature and initial volume of th...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2011-12-01
|
| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0006_application-of-quantitative-approach-focused-on-the-competition-of-lactic-acid-bacteria-culture-with-coagulase.php |
| _version_ | 1797899772460269568 |
|---|---|
| author | Ľubomír Valík Alžbeta Medveďová Denisa Liptáková |
| author_facet | Ľubomír Valík Alžbeta Medveďová Denisa Liptáková |
| author_sort | Ľubomír Valík |
| collection | DOAJ |
| description | A quantitative analysis of Staphylococcus aureus growth was carried out in milk fermented with the mesophilic mixed-strain culture of lactic acid bacteria (LAB), in relation to the conditions prevailing during artisanal ewes' lump cheese production. Both the temperature and initial volume of the culture had a dramatic effect on the behaviour of the S. aureus strain under study. Depending on the conditions, the growth, reaching maximal population density (Nmax) and a strong inhibition of S. aureus were observed. Regression analysis of the results enabled us to explain the relationships between the parameters such as the growth or inhibition rate, lag phase and Nmax-N0 and the temperature and initial culture density on the other hand. The surface methodology enabled to show that the rate of S. aureus inhibition (rinh) was affected by the temperature and volume of LAB inoculum following the equation: rinh =-0.1302 + 0.02325 × T - 0.000975 × T 2 - 0.00001 × T 2 × V 02 (R = 0.965). The fact that S. aureus increased in number mostly only by about 1 log and did not reach 106 CFU/ml during our model experiments was the most useful for good manufacturing practice. The knowledge obtained in this study concerned with the quantitative behaviour of S. aureus including the information on the inhibition at the selected environmental factors (temperature, inoculum size, and pH) is essential for the improvement of the fermentation process, quality and safety of artisanal ewes' cheese production used as a raw material for industrial Bryndza cheese production. |
| first_indexed | 2024-04-10T08:35:18Z |
| format | Article |
| id | doaj.art-d0f9e63c697246f9a89bf523da5f5086 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:18Z |
| publishDate | 2011-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-d0f9e63c697246f9a89bf523da5f50862023-02-23T03:27:40ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172011-12-0129Special IssueS23S2910.17221/264/2011-CJFScjf-201110-0006Application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase-positive staphylococci under the conditions related to artisanal cheese fermentationĽubomír Valík0Alžbeta Medveďová1Denisa Liptáková2Department of Nutrition and Food Quality Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovak RepublicDepartment of Nutrition and Food Quality Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovak RepublicDepartment of Nutrition and Food Quality Assessment, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovak RepublicA quantitative analysis of Staphylococcus aureus growth was carried out in milk fermented with the mesophilic mixed-strain culture of lactic acid bacteria (LAB), in relation to the conditions prevailing during artisanal ewes' lump cheese production. Both the temperature and initial volume of the culture had a dramatic effect on the behaviour of the S. aureus strain under study. Depending on the conditions, the growth, reaching maximal population density (Nmax) and a strong inhibition of S. aureus were observed. Regression analysis of the results enabled us to explain the relationships between the parameters such as the growth or inhibition rate, lag phase and Nmax-N0 and the temperature and initial culture density on the other hand. The surface methodology enabled to show that the rate of S. aureus inhibition (rinh) was affected by the temperature and volume of LAB inoculum following the equation: rinh =-0.1302 + 0.02325 × T - 0.000975 × T 2 - 0.00001 × T 2 × V 02 (R = 0.965). The fact that S. aureus increased in number mostly only by about 1 log and did not reach 106 CFU/ml during our model experiments was the most useful for good manufacturing practice. The knowledge obtained in this study concerned with the quantitative behaviour of S. aureus including the information on the inhibition at the selected environmental factors (temperature, inoculum size, and pH) is essential for the improvement of the fermentation process, quality and safety of artisanal ewes' cheese production used as a raw material for industrial Bryndza cheese production.https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0006_application-of-quantitative-approach-focused-on-the-competition-of-lactic-acid-bacteria-culture-with-coagulase.phpstaphylococcus aureuslactic acid bacteriagrowth competitionsurface methodology |
| spellingShingle | Ľubomír Valík Alžbeta Medveďová Denisa Liptáková Application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase-positive staphylococci under the conditions related to artisanal cheese fermentation Czech Journal of Food Sciences staphylococcus aureus lactic acid bacteria growth competition surface methodology |
| title | Application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase-positive staphylococci under the conditions related to artisanal cheese fermentation |
| title_full | Application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase-positive staphylococci under the conditions related to artisanal cheese fermentation |
| title_fullStr | Application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase-positive staphylococci under the conditions related to artisanal cheese fermentation |
| title_full_unstemmed | Application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase-positive staphylococci under the conditions related to artisanal cheese fermentation |
| title_short | Application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase-positive staphylococci under the conditions related to artisanal cheese fermentation |
| title_sort | application of quantitative approach focused on the competition of lactic acid bacteria culture with coagulase positive staphylococci under the conditions related to artisanal cheese fermentation |
| topic | staphylococcus aureus lactic acid bacteria growth competition surface methodology |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201110-0006_application-of-quantitative-approach-focused-on-the-competition-of-lactic-acid-bacteria-culture-with-coagulase.php |
| work_keys_str_mv | AT lubomirvalik applicationofquantitativeapproachfocusedonthecompetitionoflacticacidbacteriaculturewithcoagulasepositivestaphylococciundertheconditionsrelatedtoartisanalcheesefermentation AT alzbetamedvedova applicationofquantitativeapproachfocusedonthecompetitionoflacticacidbacteriaculturewithcoagulasepositivestaphylococciundertheconditionsrelatedtoartisanalcheesefermentation AT denisaliptakova applicationofquantitativeapproachfocusedonthecompetitionoflacticacidbacteriaculturewithcoagulasepositivestaphylococciundertheconditionsrelatedtoartisanalcheesefermentation |