Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS
Gold nanoparticles (AuNPs) are increasingly studied for cancer treatment purposes, as they can potentially improve both control and efficiency of the treatment. Intensive research is conducted in vitro on rodent and human cell lines to objectify the gain of combining AuNPs with cancer treatment and...
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| Format: | Article |
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MDPI AG
2019-01-01
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| Online Access: | http://www.mdpi.com/2297-8739/6/1/3 |
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| author | Johanna Noireaux Romain Grall Marie Hullo Sylvie Chevillard Caroline Oster Emilie Brun Cécile Sicard-Roselli Katrin Loeschner Paola Fisicaro |
| author_facet | Johanna Noireaux Romain Grall Marie Hullo Sylvie Chevillard Caroline Oster Emilie Brun Cécile Sicard-Roselli Katrin Loeschner Paola Fisicaro |
| author_sort | Johanna Noireaux |
| collection | DOAJ |
| description | Gold nanoparticles (AuNPs) are increasingly studied for cancer treatment purposes, as they can potentially improve both control and efficiency of the treatment. Intensive research is conducted in vitro on rodent and human cell lines to objectify the gain of combining AuNPs with cancer treatment and to understand their mechanisms of action. However, using nanoparticles in such studies requires thorough knowledge of their cellular uptake. In this study, we optimized single particle ICPMS (sp-ICPMS) analysis to qualify and quantify intracellular AuNP content after exposure of in vitro human breast cancer cell lines. To this aim, cells were treated with an alkaline digestion method with 5% TMAH, allowing the detection of gold with a yield of 97% on average. Results showed that under our experimental conditions, the AuNP size distribution appeared to be unchanged after internalization and that the uptake of particles depended on the cell line and on the exposure duration. Finally, the comparison of the particle numbers per cell with the estimates based on the gold masses showed excellent agreement, confirming the validity of the sp-ICPMS particle measurements in such complex samples. |
| first_indexed | 2024-04-11T22:50:00Z |
| format | Article |
| id | doaj.art-d0fb503cca4e4a78983c4567f1a00aeb |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-04-11T22:50:00Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-d0fb503cca4e4a78983c4567f1a00aeb2022-12-22T03:58:37ZengMDPI AGSeparations2297-87392019-01-0161310.3390/separations6010003separations6010003Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMSJohanna Noireaux0Romain Grall1Marie Hullo2Sylvie Chevillard3Caroline Oster4Emilie Brun5Cécile Sicard-Roselli6Katrin Loeschner7Paola Fisicaro8Department for biomedical and inorganic chemistry, LNE, 75015 Paris, FranceExperimental cancerology laboratory, CEA, 92260 Fontenay-aux-Roses, FranceExperimental cancerology laboratory, CEA, 92260 Fontenay-aux-Roses, FranceExperimental cancerology laboratory, CEA, 92260 Fontenay-aux-Roses, FranceDepartment for biomedical and inorganic chemistry, LNE, 75015 Paris, FranceLaboratoire de Chimie Physique, UMR CNRS 8000, Université Paris Sud, 91405 Orsay, FranceLaboratoire de Chimie Physique, UMR CNRS 8000, Université Paris Sud, 91405 Orsay, FranceNational Food Institute, Technical University of Denmark, 2800 Kgs. Lyngby, DenmarkDepartment for biomedical and inorganic chemistry, LNE, 75015 Paris, FranceGold nanoparticles (AuNPs) are increasingly studied for cancer treatment purposes, as they can potentially improve both control and efficiency of the treatment. Intensive research is conducted in vitro on rodent and human cell lines to objectify the gain of combining AuNPs with cancer treatment and to understand their mechanisms of action. However, using nanoparticles in such studies requires thorough knowledge of their cellular uptake. In this study, we optimized single particle ICPMS (sp-ICPMS) analysis to qualify and quantify intracellular AuNP content after exposure of in vitro human breast cancer cell lines. To this aim, cells were treated with an alkaline digestion method with 5% TMAH, allowing the detection of gold with a yield of 97% on average. Results showed that under our experimental conditions, the AuNP size distribution appeared to be unchanged after internalization and that the uptake of particles depended on the cell line and on the exposure duration. Finally, the comparison of the particle numbers per cell with the estimates based on the gold masses showed excellent agreement, confirming the validity of the sp-ICPMS particle measurements in such complex samples.http://www.mdpi.com/2297-8739/6/1/3sp-ICPMSgold nanoparticlestumor cellsnanoparticle uptake |
| spellingShingle | Johanna Noireaux Romain Grall Marie Hullo Sylvie Chevillard Caroline Oster Emilie Brun Cécile Sicard-Roselli Katrin Loeschner Paola Fisicaro Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS Separations sp-ICPMS gold nanoparticles tumor cells nanoparticle uptake |
| title | Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS |
| title_full | Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS |
| title_fullStr | Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS |
| title_full_unstemmed | Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS |
| title_short | Gold Nanoparticle Uptake in Tumor Cells: Quantification and Size Distribution by sp-ICPMS |
| title_sort | gold nanoparticle uptake in tumor cells quantification and size distribution by sp icpms |
| topic | sp-ICPMS gold nanoparticles tumor cells nanoparticle uptake |
| url | http://www.mdpi.com/2297-8739/6/1/3 |
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