A Pilot Study Exploiting the Industrialization Potential of Solid Lipid Nanoparticle-Based Metered-Dose Inhalers
Background: Delivery of inhalable nanoparticles through metered-dose inhalers (MDI) is a promising approach to treat lung disease such as asthma and chronic obstructive pulmonary disease. Nanocoating of the inhalable nanoparticles helps in stability and cellular uptake enhancement but complicates th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/3/866 |
| _version_ | 1797609526015295488 |
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| author | Lei Shu Wenhua Wang Chon-iong Ng Xuejuan Zhang Ying Huang Chuanbin Wu Xin Pan Zhengwei Huang |
| author_facet | Lei Shu Wenhua Wang Chon-iong Ng Xuejuan Zhang Ying Huang Chuanbin Wu Xin Pan Zhengwei Huang |
| author_sort | Lei Shu |
| collection | DOAJ |
| description | Background: Delivery of inhalable nanoparticles through metered-dose inhalers (MDI) is a promising approach to treat lung disease such as asthma and chronic obstructive pulmonary disease. Nanocoating of the inhalable nanoparticles helps in stability and cellular uptake enhancement but complicates the production process. Thus, it is meaningful to accelerate the translation process of MDI encapsulating inhalable nanoparticles with nanocoating structure. Methods: In this study, solid lipid nanoparticles (SLN) are selected as a model inhalable nanoparticle system. An established reverse microemulsion strategy was utilized to explore the industrialization potential of SLN-based MDI. Three categories of nanocoating with the functions of stabilization (by Poloxamer 188, encoded as SLN(0)), cellular uptake enhancement (by cetyltrimethylammonium bromide, encoded as SLN(+)), and targetability (by hyaluronic acid, encoded as SLN(−)) were constructed upon SLN, whose particle size distribution and zeta-potential were characterized. Subsequently, SLN were loaded into MDI, and evaluated for the processing reliability, physicochemical nature, formulation stability, and biocompatibility. Results: The results elucidated that three types of SLN-based MDI were successfully fabricated with good reproducibility and stability. Regarding safety, SLN(0) and SLN(−) showed negligible cytotoxicity on cellular level. Conclusions: This work serves as a pilot study for the scale-up of SLN-based MDI, and could be useful for the future development of inhalable nanoparticles. |
| first_indexed | 2024-03-11T06:02:39Z |
| format | Article |
| id | doaj.art-d103d0b66dbc46f4b0dc2ef1cf108875 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T06:02:39Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-d103d0b66dbc46f4b0dc2ef1cf1088752023-11-17T13:15:29ZengMDPI AGPharmaceutics1999-49232023-03-0115386610.3390/pharmaceutics15030866A Pilot Study Exploiting the Industrialization Potential of Solid Lipid Nanoparticle-Based Metered-Dose InhalersLei Shu0Wenhua Wang1Chon-iong Ng2Xuejuan Zhang3Ying Huang4Chuanbin Wu5Xin Pan6Zhengwei Huang7College of Pharmacy, Jinan University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaCollege of Pharmacy, Jinan University, Guangzhou 510006, ChinaCollege of Pharmacy, Jinan University, Guangzhou 510006, ChinaCollege of Pharmacy, Jinan University, Guangzhou 510006, ChinaCollege of Pharmacy, Jinan University, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510275, ChinaCollege of Pharmacy, Jinan University, Guangzhou 510006, ChinaBackground: Delivery of inhalable nanoparticles through metered-dose inhalers (MDI) is a promising approach to treat lung disease such as asthma and chronic obstructive pulmonary disease. Nanocoating of the inhalable nanoparticles helps in stability and cellular uptake enhancement but complicates the production process. Thus, it is meaningful to accelerate the translation process of MDI encapsulating inhalable nanoparticles with nanocoating structure. Methods: In this study, solid lipid nanoparticles (SLN) are selected as a model inhalable nanoparticle system. An established reverse microemulsion strategy was utilized to explore the industrialization potential of SLN-based MDI. Three categories of nanocoating with the functions of stabilization (by Poloxamer 188, encoded as SLN(0)), cellular uptake enhancement (by cetyltrimethylammonium bromide, encoded as SLN(+)), and targetability (by hyaluronic acid, encoded as SLN(−)) were constructed upon SLN, whose particle size distribution and zeta-potential were characterized. Subsequently, SLN were loaded into MDI, and evaluated for the processing reliability, physicochemical nature, formulation stability, and biocompatibility. Results: The results elucidated that three types of SLN-based MDI were successfully fabricated with good reproducibility and stability. Regarding safety, SLN(0) and SLN(−) showed negligible cytotoxicity on cellular level. Conclusions: This work serves as a pilot study for the scale-up of SLN-based MDI, and could be useful for the future development of inhalable nanoparticles.https://www.mdpi.com/1999-4923/15/3/866inhalationsolid lipid nanoparticlemetered-dose inhalerpilot studynanocoating |
| spellingShingle | Lei Shu Wenhua Wang Chon-iong Ng Xuejuan Zhang Ying Huang Chuanbin Wu Xin Pan Zhengwei Huang A Pilot Study Exploiting the Industrialization Potential of Solid Lipid Nanoparticle-Based Metered-Dose Inhalers Pharmaceutics inhalation solid lipid nanoparticle metered-dose inhaler pilot study nanocoating |
| title | A Pilot Study Exploiting the Industrialization Potential of Solid Lipid Nanoparticle-Based Metered-Dose Inhalers |
| title_full | A Pilot Study Exploiting the Industrialization Potential of Solid Lipid Nanoparticle-Based Metered-Dose Inhalers |
| title_fullStr | A Pilot Study Exploiting the Industrialization Potential of Solid Lipid Nanoparticle-Based Metered-Dose Inhalers |
| title_full_unstemmed | A Pilot Study Exploiting the Industrialization Potential of Solid Lipid Nanoparticle-Based Metered-Dose Inhalers |
| title_short | A Pilot Study Exploiting the Industrialization Potential of Solid Lipid Nanoparticle-Based Metered-Dose Inhalers |
| title_sort | pilot study exploiting the industrialization potential of solid lipid nanoparticle based metered dose inhalers |
| topic | inhalation solid lipid nanoparticle metered-dose inhaler pilot study nanocoating |
| url | https://www.mdpi.com/1999-4923/15/3/866 |
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