Epididymis incision as a method to collect epididymal sperm cells in alpacas
Abstract Background Epididymal sperm can be collected post‐castration or post‐mortem. This method has already been described for alpaca (Vicugna pacos), but little data are available on success rate and the influence of diluent on it. Objective The aim was to investigate the effectiveness of epididy...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.681 |
| _version_ | 1798025182942593024 |
|---|---|
| author | Marlen Auer Henrik Wagner Klaus Failing Axel Wehrend |
| author_facet | Marlen Auer Henrik Wagner Klaus Failing Axel Wehrend |
| author_sort | Marlen Auer |
| collection | DOAJ |
| description | Abstract Background Epididymal sperm can be collected post‐castration or post‐mortem. This method has already been described for alpaca (Vicugna pacos), but little data are available on success rate and the influence of diluent on it. Objective The aim was to investigate the effectiveness of epididymis incision as an extraction method. Method Post‐castration epididymides (n = 78) were incised and semen was collected from cut surfaces. Further sperm were flushed with two semen extenders. For the left epididymis, a diluent without animal proteins and for the right, a diluent with egg yolk was utilised. Collected sperms were immediately spermatologically examined. Results Due to incorrect measurements, the samples of seven epididymides were not analysed. An evaluation was possible in 58 samples. Average density was 108.80 ± 83.28 million/mL and motility was 53.30 ± 18.17%. On average, 76.70 ± 11.60% of the sperm were vital in eosin‐stained specimens. In the hypoosmotic swelling test, an average of 69.50 ± 10.48% of the sperm had an intact plasma membrane. Semen extender had no effect on spermatological parameters (p > 0.05). Overall success rate of sperm recovery was 83%. Extraction of epididymal sperm was possible in 68.80% of the 2‐year‐old males. The 13 epididymides from which no sperm recovery was possible were histologically examined and for 10, there was no histological evidence of sperm. The corrected success rate was 95.60%. Conclusion Testicular volume (p = 0.0453), but not age (p = 0.62), had an effect on the probability of obtaining sperm. |
| first_indexed | 2024-04-11T18:14:38Z |
| format | Article |
| id | doaj.art-d10957cd0b8e425496228a49be7ed5dc |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-04-11T18:14:38Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-d10957cd0b8e425496228a49be7ed5dc2022-12-22T04:09:59ZengWileyVeterinary Medicine and Science2053-10952022-01-018115716310.1002/vms3.681Epididymis incision as a method to collect epididymal sperm cells in alpacasMarlen Auer0Henrik Wagner1Klaus Failing2Axel Wehrend3Clinic for Veterinary Obstetrics, Gynaecology and Andrology for Large and Small Animals with Veterinary Ambulance, Faculty of Veterinary Medicine Justus‐Liebig‐University Giessen Giessen GermanyClinic for Veterinary Obstetrics, Gynaecology and Andrology for Large and Small Animals with Veterinary Ambulance, Faculty of Veterinary Medicine Justus‐Liebig‐University Giessen Giessen GermanyUnit for Biomathematics and Data Processing Justus‐Liebig‐University Giessen Giessen GermanyClinic for Veterinary Obstetrics, Gynaecology and Andrology for Large and Small Animals with Veterinary Ambulance, Faculty of Veterinary Medicine Justus‐Liebig‐University Giessen Giessen GermanyAbstract Background Epididymal sperm can be collected post‐castration or post‐mortem. This method has already been described for alpaca (Vicugna pacos), but little data are available on success rate and the influence of diluent on it. Objective The aim was to investigate the effectiveness of epididymis incision as an extraction method. Method Post‐castration epididymides (n = 78) were incised and semen was collected from cut surfaces. Further sperm were flushed with two semen extenders. For the left epididymis, a diluent without animal proteins and for the right, a diluent with egg yolk was utilised. Collected sperms were immediately spermatologically examined. Results Due to incorrect measurements, the samples of seven epididymides were not analysed. An evaluation was possible in 58 samples. Average density was 108.80 ± 83.28 million/mL and motility was 53.30 ± 18.17%. On average, 76.70 ± 11.60% of the sperm were vital in eosin‐stained specimens. In the hypoosmotic swelling test, an average of 69.50 ± 10.48% of the sperm had an intact plasma membrane. Semen extender had no effect on spermatological parameters (p > 0.05). Overall success rate of sperm recovery was 83%. Extraction of epididymal sperm was possible in 68.80% of the 2‐year‐old males. The 13 epididymides from which no sperm recovery was possible were histologically examined and for 10, there was no histological evidence of sperm. The corrected success rate was 95.60%. Conclusion Testicular volume (p = 0.0453), but not age (p = 0.62), had an effect on the probability of obtaining sperm.https://doi.org/10.1002/vms3.681alpacaepididymisextraction methodsperm recoveryVicugna pacos |
| spellingShingle | Marlen Auer Henrik Wagner Klaus Failing Axel Wehrend Epididymis incision as a method to collect epididymal sperm cells in alpacas Veterinary Medicine and Science alpaca epididymis extraction method sperm recovery Vicugna pacos |
| title | Epididymis incision as a method to collect epididymal sperm cells in alpacas |
| title_full | Epididymis incision as a method to collect epididymal sperm cells in alpacas |
| title_fullStr | Epididymis incision as a method to collect epididymal sperm cells in alpacas |
| title_full_unstemmed | Epididymis incision as a method to collect epididymal sperm cells in alpacas |
| title_short | Epididymis incision as a method to collect epididymal sperm cells in alpacas |
| title_sort | epididymis incision as a method to collect epididymal sperm cells in alpacas |
| topic | alpaca epididymis extraction method sperm recovery Vicugna pacos |
| url | https://doi.org/10.1002/vms3.681 |
| work_keys_str_mv | AT marlenauer epididymisincisionasamethodtocollectepididymalspermcellsinalpacas AT henrikwagner epididymisincisionasamethodtocollectepididymalspermcellsinalpacas AT klausfailing epididymisincisionasamethodtocollectepididymalspermcellsinalpacas AT axelwehrend epididymisincisionasamethodtocollectepididymalspermcellsinalpacas |