In Vitro Assay of Translation Inhibition by Trichothecenes Using a Commercially Available System
Trichothecenes are a family of major secondary metabolites produced by some common filamentous fungi, including plant pathogenic and entomopathogenic fungi. It may be considered difficult to conduct a comparison between the toxicities of trichothecenes with consideration of different conditions and...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/10/696 |
| _version_ | 1797513093010423808 |
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| author | Takahito Toyotome Katsuhiko Kamei |
| author_facet | Takahito Toyotome Katsuhiko Kamei |
| author_sort | Takahito Toyotome |
| collection | DOAJ |
| description | Trichothecenes are a family of major secondary metabolites produced by some common filamentous fungi, including plant pathogenic and entomopathogenic fungi. It may be considered difficult to conduct a comparison between the toxicities of trichothecenes with consideration of different conditions and cell lines. In the current study, we developed an in vitro assay based on a commercially available system to estimate the translation inhibition, that is, the main toxicity, of trichothecenes. The assay was applied to estimate the inhibition of protein synthesis by trichothecenes. Initially, we examined the assay using trichothecene dissolved in water followed by an assessment of trichothecene solutions dissolved in acetonitrile. The obtained data showed that the assay tolerated the small amount of acetonitrile. The assay examined in this study has the advantages of a short operation time (one day), ease of use, and data stability, as it is a non-cell-based assay whose components are commercially available. It is expected that this assay will contribute to the evaluation of the toxicity of a vast number of trichothecenes. |
| first_indexed | 2024-03-10T06:10:49Z |
| format | Article |
| id | doaj.art-d10c27b869fa4af382660715cab8e3f3 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T06:10:49Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d10c27b869fa4af382660715cab8e3f32023-11-22T20:13:03ZengMDPI AGToxins2072-66512021-10-01131069610.3390/toxins13100696In Vitro Assay of Translation Inhibition by Trichothecenes Using a Commercially Available SystemTakahito Toyotome0Katsuhiko Kamei1Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, JapanMedical Mycology Research Center, Chiba University, Chiba 260-8673, JapanTrichothecenes are a family of major secondary metabolites produced by some common filamentous fungi, including plant pathogenic and entomopathogenic fungi. It may be considered difficult to conduct a comparison between the toxicities of trichothecenes with consideration of different conditions and cell lines. In the current study, we developed an in vitro assay based on a commercially available system to estimate the translation inhibition, that is, the main toxicity, of trichothecenes. The assay was applied to estimate the inhibition of protein synthesis by trichothecenes. Initially, we examined the assay using trichothecene dissolved in water followed by an assessment of trichothecene solutions dissolved in acetonitrile. The obtained data showed that the assay tolerated the small amount of acetonitrile. The assay examined in this study has the advantages of a short operation time (one day), ease of use, and data stability, as it is a non-cell-based assay whose components are commercially available. It is expected that this assay will contribute to the evaluation of the toxicity of a vast number of trichothecenes.https://www.mdpi.com/2072-6651/13/10/696trichothecenesin vitro assaycell-free assayprotein synthesis inhibition |
| spellingShingle | Takahito Toyotome Katsuhiko Kamei In Vitro Assay of Translation Inhibition by Trichothecenes Using a Commercially Available System Toxins trichothecenes in vitro assay cell-free assay protein synthesis inhibition |
| title | In Vitro Assay of Translation Inhibition by Trichothecenes Using a Commercially Available System |
| title_full | In Vitro Assay of Translation Inhibition by Trichothecenes Using a Commercially Available System |
| title_fullStr | In Vitro Assay of Translation Inhibition by Trichothecenes Using a Commercially Available System |
| title_full_unstemmed | In Vitro Assay of Translation Inhibition by Trichothecenes Using a Commercially Available System |
| title_short | In Vitro Assay of Translation Inhibition by Trichothecenes Using a Commercially Available System |
| title_sort | in vitro assay of translation inhibition by trichothecenes using a commercially available system |
| topic | trichothecenes in vitro assay cell-free assay protein synthesis inhibition |
| url | https://www.mdpi.com/2072-6651/13/10/696 |
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