The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation
Late implant failures, caused by the inflammation of surrounding tissues are a problem in implant dentistry. The path of bacterial transmission from teeth to implants is not completely understood. Therefore, the purpose of this study was to analyze intraindividual bacterial transmission characterizi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-03-01
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| Series: | Dentistry Journal |
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| Online Access: | http://www.mdpi.com/2304-6767/3/2/24 |
| _version_ | 1798024233864921088 |
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| author | Meike Luise Jakobi Sascha Nico Stumpp Meike Stiesch Jörg Eberhard Wieland Heuer |
| author_facet | Meike Luise Jakobi Sascha Nico Stumpp Meike Stiesch Jörg Eberhard Wieland Heuer |
| author_sort | Meike Luise Jakobi |
| collection | DOAJ |
| description | Late implant failures, caused by the inflammation of surrounding tissues are a problem in implant dentistry. The path of bacterial transmission from teeth to implants is not completely understood. Therefore, the purpose of this study was to analyze intraindividual bacterial transmission characterizing subgingival microbiomes in teeth and implants, both in healthy subjects and in those with signs of periodontitis or peri-implantitis. Samples of peri-implant and dental sulcus fluid were collected. To identify the predominant microbiota, amplified fragments of bacterial 16S rRNA gene were separated by single strand conformation polymorphism analysis, sequenced and taxonomically classified. A total of 25 different predominant genera were found in the diseased group and 14 genera in the healthy group. Species richness did not differ significantly between implants, neighboring teeth and teeth with largest probing depth in the diseased group. Additionally, no differences between teeth and implants in the healthy group were detected. In contrast, microbial diversity varied between the different sampling points. Species richness is similar in healthy and diseased sites, but the composition of the bacterial community differed within the individual subjects. The underlying analyses strongly suggest that complete transmission from neighboring teeth to implants is unlikely. |
| first_indexed | 2024-04-11T17:59:06Z |
| format | Article |
| id | doaj.art-d10c5a0d3a044eeea2d57484205948d4 |
| institution | Directory Open Access Journal |
| issn | 2304-6767 |
| language | English |
| last_indexed | 2024-04-11T17:59:06Z |
| publishDate | 2015-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-d10c5a0d3a044eeea2d57484205948d42022-12-22T04:10:35ZengMDPI AGDentistry Journal2304-67672015-03-0132244210.3390/dj3020024dj3020024The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of InflammationMeike Luise Jakobi0Sascha Nico Stumpp1Meike Stiesch2Jörg Eberhard3Wieland Heuer4Private Dental Practice, Hildesheim 31134, GermanyDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, GermanyDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, GermanyDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, GermanyDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Strasse 1, Hannover 30625, GermanyLate implant failures, caused by the inflammation of surrounding tissues are a problem in implant dentistry. The path of bacterial transmission from teeth to implants is not completely understood. Therefore, the purpose of this study was to analyze intraindividual bacterial transmission characterizing subgingival microbiomes in teeth and implants, both in healthy subjects and in those with signs of periodontitis or peri-implantitis. Samples of peri-implant and dental sulcus fluid were collected. To identify the predominant microbiota, amplified fragments of bacterial 16S rRNA gene were separated by single strand conformation polymorphism analysis, sequenced and taxonomically classified. A total of 25 different predominant genera were found in the diseased group and 14 genera in the healthy group. Species richness did not differ significantly between implants, neighboring teeth and teeth with largest probing depth in the diseased group. Additionally, no differences between teeth and implants in the healthy group were detected. In contrast, microbial diversity varied between the different sampling points. Species richness is similar in healthy and diseased sites, but the composition of the bacterial community differed within the individual subjects. The underlying analyses strongly suggest that complete transmission from neighboring teeth to implants is unlikely.http://www.mdpi.com/2304-6767/3/2/24bacterial biofilmsdental implantsmicrobial diversitysingle strand conformation polymorphism/SSCPperi-implantitis |
| spellingShingle | Meike Luise Jakobi Sascha Nico Stumpp Meike Stiesch Jörg Eberhard Wieland Heuer The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation Dentistry Journal bacterial biofilms dental implants microbial diversity single strand conformation polymorphism/SSCP peri-implantitis |
| title | The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation |
| title_full | The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation |
| title_fullStr | The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation |
| title_full_unstemmed | The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation |
| title_short | The Peri-Implant and Periodontal Microbiota in Patients with and without Clinical Signs of Inflammation |
| title_sort | peri implant and periodontal microbiota in patients with and without clinical signs of inflammation |
| topic | bacterial biofilms dental implants microbial diversity single strand conformation polymorphism/SSCP peri-implantitis |
| url | http://www.mdpi.com/2304-6767/3/2/24 |
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